Bradykinin stimulation of electrogenic ion transport in epithelial layers of cultured human endometrium

C J Matthews, G T McEwan, C P Redfern, E J Thomas, B H Hirst

Research output: Contribution to journalArticle

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Abstract

Primary cultures of glandular endometrial epithelial cells grown on permeable supports formed monolayers with a high transepithelial electrical resistance [1096 +/- 83 omega.cm2 (n = 34)] and displayed electrogenic ion transport as demonstrated by an inward short circuit current (Isc; 20 +/- 2 microA/cm2). Bradykinin, 10(-8)-10(-6) M, added to either the basolateral or apical solutions enhanced the inward ISC. The concentration-response curves for bradykinin were bell-shaped in nature. The ISC response was more sensitive to apical addition of bradykinin and the maximum response was also greater with apical bradykinin. The increases in ISC were accompanied by two- to three-fold increases in transepithelial conductance. Apical addition of amiloride, 10(-4) M, reduced the unstimulated ISC by 80%. In the presence of amiloride, the response to both apical and basolateral bradykinin was reduced by > 50% in 8 out of 18 layers, and the mean response was reduced by approximately 25%. The data are consistent with a physiological role for bradykinin in the control of the intrauterine electrolyte environment, mediated in part by enhanced Na+ absorption.
Original languageEnglish
Pages (from-to)401-3
Number of pages3
JournalPflugers Archiv : European Journal of Physiology
Volume422
Issue number4
Publication statusPublished - Jan 1993

Fingerprint

Ion Transport
Bradykinin
Endometrium
Ions
Amiloride
Acoustic impedance
Electric Impedance
Short circuit currents
Electrolytes
Monolayers
Epithelial Cells

Keywords

  • Amiloride
  • Bradykinin
  • Culture Techniques
  • Endometrium
  • Epithelium
  • Female
  • Humans
  • Ion Transport
  • Membrane Potentials

Cite this

Bradykinin stimulation of electrogenic ion transport in epithelial layers of cultured human endometrium. / Matthews, C J; McEwan, G T; Redfern, C P; Thomas, E J; Hirst, B H.

In: Pflugers Archiv : European Journal of Physiology, Vol. 422, No. 4, 01.1993, p. 401-3.

Research output: Contribution to journalArticle

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abstract = "Primary cultures of glandular endometrial epithelial cells grown on permeable supports formed monolayers with a high transepithelial electrical resistance [1096 +/- 83 omega.cm2 (n = 34)] and displayed electrogenic ion transport as demonstrated by an inward short circuit current (Isc; 20 +/- 2 microA/cm2). Bradykinin, 10(-8)-10(-6) M, added to either the basolateral or apical solutions enhanced the inward ISC. The concentration-response curves for bradykinin were bell-shaped in nature. The ISC response was more sensitive to apical addition of bradykinin and the maximum response was also greater with apical bradykinin. The increases in ISC were accompanied by two- to three-fold increases in transepithelial conductance. Apical addition of amiloride, 10(-4) M, reduced the unstimulated ISC by 80{\%}. In the presence of amiloride, the response to both apical and basolateral bradykinin was reduced by > 50{\%} in 8 out of 18 layers, and the mean response was reduced by approximately 25{\%}. The data are consistent with a physiological role for bradykinin in the control of the intrauterine electrolyte environment, mediated in part by enhanced Na+ absorption.",
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T1 - Bradykinin stimulation of electrogenic ion transport in epithelial layers of cultured human endometrium

AU - Matthews, C J

AU - McEwan, G T

AU - Redfern, C P

AU - Thomas, E J

AU - Hirst, B H

PY - 1993/1

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N2 - Primary cultures of glandular endometrial epithelial cells grown on permeable supports formed monolayers with a high transepithelial electrical resistance [1096 +/- 83 omega.cm2 (n = 34)] and displayed electrogenic ion transport as demonstrated by an inward short circuit current (Isc; 20 +/- 2 microA/cm2). Bradykinin, 10(-8)-10(-6) M, added to either the basolateral or apical solutions enhanced the inward ISC. The concentration-response curves for bradykinin were bell-shaped in nature. The ISC response was more sensitive to apical addition of bradykinin and the maximum response was also greater with apical bradykinin. The increases in ISC were accompanied by two- to three-fold increases in transepithelial conductance. Apical addition of amiloride, 10(-4) M, reduced the unstimulated ISC by 80%. In the presence of amiloride, the response to both apical and basolateral bradykinin was reduced by > 50% in 8 out of 18 layers, and the mean response was reduced by approximately 25%. The data are consistent with a physiological role for bradykinin in the control of the intrauterine electrolyte environment, mediated in part by enhanced Na+ absorption.

AB - Primary cultures of glandular endometrial epithelial cells grown on permeable supports formed monolayers with a high transepithelial electrical resistance [1096 +/- 83 omega.cm2 (n = 34)] and displayed electrogenic ion transport as demonstrated by an inward short circuit current (Isc; 20 +/- 2 microA/cm2). Bradykinin, 10(-8)-10(-6) M, added to either the basolateral or apical solutions enhanced the inward ISC. The concentration-response curves for bradykinin were bell-shaped in nature. The ISC response was more sensitive to apical addition of bradykinin and the maximum response was also greater with apical bradykinin. The increases in ISC were accompanied by two- to three-fold increases in transepithelial conductance. Apical addition of amiloride, 10(-4) M, reduced the unstimulated ISC by 80%. In the presence of amiloride, the response to both apical and basolateral bradykinin was reduced by > 50% in 8 out of 18 layers, and the mean response was reduced by approximately 25%. The data are consistent with a physiological role for bradykinin in the control of the intrauterine electrolyte environment, mediated in part by enhanced Na+ absorption.

KW - Amiloride

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KW - Culture Techniques

KW - Endometrium

KW - Epithelium

KW - Female

KW - Humans

KW - Ion Transport

KW - Membrane Potentials

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JO - Pflugers Archiv : European Journal of Physiology

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