Breakdown of diazotized proteins and synthetic substrates by rumen bacterial proteases

R. J. Wallace, J. Kopecny

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Several different kinds of substrate were used to investigate the proteolytic activity of rumen bacteria and of proteases released from rumen bacteria by blending ("coat proteases"). These substrates included diazotized feed proteins and diazotized soluble and insoluble pure proteins. It was concluded that, while solubility was an important factor, the secondary and tertiary structure of a protein had a major influence on its rate of digestion. The resistance of elastin congo red to digestion indicated that similar fibrous proteins in plant material might resist proteolytic attack by rumen bacteria. Coat proteases had a broad specificity, including several exo- and endopeptidase activities, as determined by using synthetic peptide substrates.
Original languageEnglish
Pages (from-to)212-217
Number of pages6
JournalApplied and Environmental Microbiology
Volume45
Issue number1
Publication statusPublished - Jan 1983

Fingerprint

Rumen
rumen bacteria
rumen
Peptide Hydrolases
proteinases
Bacteria
substrate
protein
Digestion
Scleroproteins
Exopeptidases
bacterium
digestion
Congo Red
Endopeptidases
Proteins
proteins
Elastin
protein tertiary structure
Tertiary Protein Structure

Keywords

  • proteinase

Cite this

Breakdown of diazotized proteins and synthetic substrates by rumen bacterial proteases. / Wallace, R. J.; Kopecny, J.

In: Applied and Environmental Microbiology, Vol. 45, No. 1, 01.1983, p. 212-217.

Research output: Contribution to journalArticle

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