Brown adipose tissue is the key depot for glucose clearance in microbiota depleted mice

Min Li, Li Li, Baoguo Li, Catherine Hambly, Guanlin Wang, Yingga Wu, Zengguang Jin, Anyongqi Wang, Chaoqun Niu, Christian Wolfrum, John R. Speakman*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Gut microbiota deficient mice demonstrate accelerated glucose clearance. However, which tissues are responsible for the upregulated glucose uptake remains unresolved, with different studies suggesting that browning of white adipose tissue, or modulated hepatic gluconeogenesis, may be related to enhanced glucose clearance when the gut microbiota is absent. Here, we investigate glucose uptake in 22 different tissues in 3 different mouse models. We find that gut microbiota depletion via treatment with antibiotic cocktails (ABX) promotes glucose uptake in brown adipose tissue (BAT) and cecum. Nevertheless, the adaptive thermogenesis and the expression of uncoupling protein 1 (UCP1) are dispensable for the increased glucose uptake and clearance. Deletion of Ucp1 expressing cells blunts the improvement of glucose clearance in ABX-treated mice. Our results indicate that BAT and cecum, but not white adipose tissue (WAT) or liver, contribute to the glucose uptake in the gut microbiota depleted mouse model and this response is dissociated from adaptive thermogenesis. Gut microbiota deficient mice demonstrate enhanced glucose clearance, but which tissues are responsible for this improvement are still unclear. Here the authors report that brown adipose tissue contributes to the enhanced glucose clearance in gut microbiota depleted mice and that this response is dissociated from adaptive thermogenesis.

Original languageEnglish
Article number4725
Number of pages13
JournalNature Communications
Volume12
Issue number1
Early online date5 Aug 2021
DOIs
Publication statusE-pub ahead of print - 5 Aug 2021

Keywords

  • MITOCHONDRIAL UNCOUPLING PROTEIN
  • DIET-INDUCED THERMOGENESIS
  • GUT MICROBIOTA
  • NONSHIVERING THERMOGENESIS
  • ENERGY-EXPENDITURE
  • INDUCED OBESITY
  • METABOLISM
  • UCP1
  • HOMEOSTASIS
  • ADIPOCYTES

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