Brown adipose tissue transplantation reverses obesity in Ob/Ob mice

Xiaomeng Liu; Siping Wang; Yilin You; Minghui Meng; Zongji Zheng; Meng Dong; Jun Lin; Qianwei Zhao; Chuanhai Zhang; Xiaoxue Yuan; Tao Hu; Lieqin Liu; Yuanyuan Huang; Lei Zhang; Dehua Wang; Jicheng Zhan; Hyuek Jong Lee; John R Speakman; Wanzhu Jin

doi:10.1210/en.2014-1598

Brown adipose tissue transplantation reverses obesity in Ob/Ob mice

Xiaomeng Liu, Siping Wang, Yilin You, Minghui Meng, Zongji Zheng, Meng Dong, Jun Lin, Qianwei Zhao, Chuanhai Zhang, Xiaoxue Yuan, Tao Hu, Lieqin Liu, Yuanyuan Huang, Lei Zhang, Dehua Wang, Jicheng Zhan, Hyuek Jong Lee, John R Speakman, Wanzhu Jin

Research output: Contribution to journal › Article › peer-review

185 Citations (Scopus)

Abstract

Increasing evidence indicates that brown adipose tissue (BAT) transplantation enhances whole-body energy metabolism in a mouse model of diet-induced obesity. However, it remains unclear whether BAT also has such beneficial effects on genetically obese mice. To address this issue, we transplanted BAT from C57/BL6 mice into the dorsal subcutaneous region of age- and sex-matched leptin deficient Ob/Ob mice. Interestingly, BAT transplantation led to a significant reduction of body weight gain with increased oxygen consumption and decreased total body fat mass, resulting in improvement of insulin resistance and liver steatosis. In addition, BAT transplantation increased the level of circulating adiponectin, whereas it reduced the levels of circulating free T3 and T4, which regulate thyroid hormone sensitivity in peripheral tissues. BAT transplantation also increased 3-adrenergic receptor and fatty acidoxidation relatedgene expressionin subcutaneous and epididymal (EP) white adipose tissue. Accordingly, BAT transplantation increased whole-body thermogenesis. Taken together our results demonstrate that BAT transplantation may reduce obesity and its related diseases by activating endogenous BAT.

Original language	English
Pages (from-to)	2461-2469
Number of pages	9
Journal	Endocrinology
Volume	156
Issue number	7
Early online date	1 Apr 2015
DOIs	https://doi.org/10.1210/en.2014-1598
Publication status	Published - Jul 2015

Bibliographical note

Acknowledgments
Author contributions include the following: W.J. is the guarantor of this work and, as such, had full access to all the data in the study and takes responsibility for the integrity of the data and the accuracy of the data analysis. W.Z.J., X.M.L., S.P.W., H.J.L., J.S.R., and Y.L.Y. wrote the manuscript; MHM performed the histology, imaging studies and Western blot; Z.J.Z. and X.M.L. performed the energy metabolism assays and the gene expression analysis; M.D., J.L., Q.W.Z., C.H.Z., X.X.Y., T.H., and L.Q.L. performed the animal surgery and the GTT and ITT studies; and L.Z., D.H.W., and J.C.Z. contributed to the discussion and editing of the manuscript.

This work was supported by the Strategic Priority Research Program (XDB13030000), the Key Research Program (KJZD-EW-L01-03) of the Chinese Academy of Sciences, a grant from the Ministry of Science and Technology of China (2012CBA01301, 2012CB944701), and the National Natural Science Foundation of China (81370951, 31171131) to W.J.

Keywords

Brown Adipose Tissue
BAT
Transplantation
Obesity
Mice
White adipose tissue
WAT

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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@article{52b56ff95e11499384f02695ed3032d5,

title = "Brown adipose tissue transplantation reverses obesity in Ob/Ob mice",

abstract = "Increasing evidence indicates that brown adipose tissue (BAT) transplantation enhances whole-body energy metabolism in a mouse model of diet-induced obesity. However, it remains unclear whether BAT also has such beneficial effects on genetically obese mice. To address this issue, we transplanted BAT from C57/BL6 mice into the dorsal subcutaneous region of age- and sex-matched leptin deficient Ob/Ob mice. Interestingly, BAT transplantation led to a significant reduction of body weight gain with increased oxygen consumption and decreased total body fat mass, resulting in improvement of insulin resistance and liver steatosis. In addition, BAT transplantation increased the level of circulating adiponectin, whereas it reduced the levels of circulating free T3 and T4, which regulate thyroid hormone sensitivity in peripheral tissues. BAT transplantation also increased 3-adrenergic receptor and fatty acidoxidation relatedgene expressionin subcutaneous and epididymal (EP) white adipose tissue. Accordingly, BAT transplantation increased whole-body thermogenesis. Taken together our results demonstrate that BAT transplantation may reduce obesity and its related diseases by activating endogenous BAT.",

keywords = "Brown Adipose Tissue, BAT, Transplantation, Obesity, Mice, White adipose tissue, WAT",

author = "Xiaomeng Liu and Siping Wang and Yilin You and Minghui Meng and Zongji Zheng and Meng Dong and Jun Lin and Qianwei Zhao and Chuanhai Zhang and Xiaoxue Yuan and Tao Hu and Lieqin Liu and Yuanyuan Huang and Lei Zhang and Dehua Wang and Jicheng Zhan and Lee, {Hyuek Jong} and Speakman, {John R} and Wanzhu Jin",

note = "Acknowledgments Author contributions include the following: W.J. is the guarantor of this work and, as such, had full access to all the data in the study and takes responsibility for the integrity of the data and the accuracy of the data analysis. W.Z.J., X.M.L., S.P.W., H.J.L., J.S.R., and Y.L.Y. wrote the manuscript; MHM performed the histology, imaging studies and Western blot; Z.J.Z. and X.M.L. performed the energy metabolism assays and the gene expression analysis; M.D., J.L., Q.W.Z., C.H.Z., X.X.Y., T.H., and L.Q.L. performed the animal surgery and the GTT and ITT studies; and L.Z., D.H.W., and J.C.Z. contributed to the discussion and editing of the manuscript. This work was supported by the Strategic Priority Research Program (XDB13030000), the Key Research Program (KJZD-EW-L01-03) of the Chinese Academy of Sciences, a grant from the Ministry of Science and Technology of China (2012CBA01301, 2012CB944701), and the National Natural Science Foundation of China (81370951, 31171131) to W.J. ",

year = "2015",

month = jul,

doi = "10.1210/en.2014-1598",

language = "English",

volume = "156",

pages = "2461--2469",

journal = "Endocrinology",

issn = "0013-7227",

publisher = "Oxford University Press (OUP)",

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TY - JOUR

T1 - Brown adipose tissue transplantation reverses obesity in Ob/Ob mice

AU - Liu, Xiaomeng

AU - Wang, Siping

AU - You, Yilin

AU - Meng, Minghui

AU - Zheng, Zongji

AU - Dong, Meng

AU - Lin, Jun

AU - Zhao, Qianwei

AU - Zhang, Chuanhai

AU - Yuan, Xiaoxue

AU - Hu, Tao

AU - Liu, Lieqin

AU - Huang, Yuanyuan

AU - Zhang, Lei

AU - Wang, Dehua

AU - Zhan, Jicheng

AU - Lee, Hyuek Jong

AU - Speakman, John R

AU - Jin, Wanzhu

N1 - Acknowledgments Author contributions include the following: W.J. is the guarantor of this work and, as such, had full access to all the data in the study and takes responsibility for the integrity of the data and the accuracy of the data analysis. W.Z.J., X.M.L., S.P.W., H.J.L., J.S.R., and Y.L.Y. wrote the manuscript; MHM performed the histology, imaging studies and Western blot; Z.J.Z. and X.M.L. performed the energy metabolism assays and the gene expression analysis; M.D., J.L., Q.W.Z., C.H.Z., X.X.Y., T.H., and L.Q.L. performed the animal surgery and the GTT and ITT studies; and L.Z., D.H.W., and J.C.Z. contributed to the discussion and editing of the manuscript. This work was supported by the Strategic Priority Research Program (XDB13030000), the Key Research Program (KJZD-EW-L01-03) of the Chinese Academy of Sciences, a grant from the Ministry of Science and Technology of China (2012CBA01301, 2012CB944701), and the National Natural Science Foundation of China (81370951, 31171131) to W.J.

PY - 2015/7

Y1 - 2015/7

N2 - Increasing evidence indicates that brown adipose tissue (BAT) transplantation enhances whole-body energy metabolism in a mouse model of diet-induced obesity. However, it remains unclear whether BAT also has such beneficial effects on genetically obese mice. To address this issue, we transplanted BAT from C57/BL6 mice into the dorsal subcutaneous region of age- and sex-matched leptin deficient Ob/Ob mice. Interestingly, BAT transplantation led to a significant reduction of body weight gain with increased oxygen consumption and decreased total body fat mass, resulting in improvement of insulin resistance and liver steatosis. In addition, BAT transplantation increased the level of circulating adiponectin, whereas it reduced the levels of circulating free T3 and T4, which regulate thyroid hormone sensitivity in peripheral tissues. BAT transplantation also increased 3-adrenergic receptor and fatty acidoxidation relatedgene expressionin subcutaneous and epididymal (EP) white adipose tissue. Accordingly, BAT transplantation increased whole-body thermogenesis. Taken together our results demonstrate that BAT transplantation may reduce obesity and its related diseases by activating endogenous BAT.

AB - Increasing evidence indicates that brown adipose tissue (BAT) transplantation enhances whole-body energy metabolism in a mouse model of diet-induced obesity. However, it remains unclear whether BAT also has such beneficial effects on genetically obese mice. To address this issue, we transplanted BAT from C57/BL6 mice into the dorsal subcutaneous region of age- and sex-matched leptin deficient Ob/Ob mice. Interestingly, BAT transplantation led to a significant reduction of body weight gain with increased oxygen consumption and decreased total body fat mass, resulting in improvement of insulin resistance and liver steatosis. In addition, BAT transplantation increased the level of circulating adiponectin, whereas it reduced the levels of circulating free T3 and T4, which regulate thyroid hormone sensitivity in peripheral tissues. BAT transplantation also increased 3-adrenergic receptor and fatty acidoxidation relatedgene expressionin subcutaneous and epididymal (EP) white adipose tissue. Accordingly, BAT transplantation increased whole-body thermogenesis. Taken together our results demonstrate that BAT transplantation may reduce obesity and its related diseases by activating endogenous BAT.

KW - Brown Adipose Tissue

KW - BAT

KW - Transplantation

KW - Obesity

KW - Mice

KW - White adipose tissue

KW - WAT

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U2 - 10.1210/en.2014-1598

DO - 10.1210/en.2014-1598

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SN - 0013-7227

VL - 156

SP - 2461

EP - 2469

JO - Endocrinology

JF - Endocrinology

IS - 7

ER -

Brown adipose tissue transplantation reverses obesity in Ob/Ob mice

Abstract

Bibliographical note

Keywords

UN SDGs

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