Brugia pahangi: characterisation of a small heat shock protein cDNA clone

F J Thompson, Samuel Allen Moore Martin, E Devaney

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Microfilariae of Brugia pahangi undergo a transition in their life cycle from the homeothermic mammalian host to the poikilothermic mosquito vector. Coincident with this switch, the developmental cycle of the microfilaria is reinitiated. When cultured at mammalian temperatures (37 degrees C), microfilariae express a complex of small heat shock proteins. In order to further characterise this group of proteins a cDNA library was constructed from heat-shocked microfilariae by reverse-transcriptase PCR and then screened with a heterologous probe. A clone which codes for a small heat shock protein was isolated and characterised in detail. Southern blot analysis identified a putative small heat shock protein multigene family. The expression of the small heat shock protein cDNA appears to be controlled at the level of RNA synthesis and is highly stage specific and temperature dependent. (C) 1996 Academic Press, Inc.

Original languageEnglish
Pages (from-to)259-266
Number of pages8
JournalExperimental Parasitology
Volume83
Issue number3
DOIs
Publication statusPublished - Aug 1996

Keywords

  • Brugia pahangi
  • microfilaria
  • small heat shock protein
  • temperature shift
  • developmental block
  • RT-PCR
  • spliced-leader
  • drosophila
  • expression
  • elegans
  • growth
  • genes
  • cells

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