Butyrate production in phylogenetically diverse Firmicutes isolated from the chicken caecum

Venessa Eeckhaut, Filip Van Immerseel, Siska Croubels, Siegrid De Baere, Freddy Haesebrouck, Richard Ducatelle, Petra Louis, Peter Vandamme

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Sixteen butyrate-producing bacteria were isolated from the caecal content of chickens and analysed phylogenetically. They did not represent a coherent phylogenetic group, but were allied to four different lineages in the Firmicutes phylum. Fourteen strains appeared to represent novel species, based on a level of <= 98.5% 16S rRNA gene sequence similarity towards their nearest validly named neighbours. The highest butyrate concentrations were produced by the strains belonging to clostridial clusters IV and XIVa, clusters which are predominant in the chicken caecal microbiota. In only one of the 16 strains tested, the butyrate kinase operon could be amplified, while the butyryl-CoA : acetate CoA-transferase gene was detected in eight strains belonging to clostridial clusters IV, XIVa and XIVb. None of the clostridial cluster XVI isolates carried this gene based on degenerate PCR analyses. However, another CoA-transferase gene more similar to propionate CoA-transferase was detected in the majority of the clostridial cluster XVI isolates. Since this gene is located directly downstream of the remaining butyrate pathway genes in several human cluster XVI bacteria, it may be involved in butyrate formation in these bacteria. The present study indicates that butyrate producers related to cluster XVI may play a more important role in the chicken gut than in the human gut.

Original languageEnglish
Pages (from-to)503-512
Number of pages10
JournalMicrobial Biotechnology
Volume4
Issue number4
Early online date17 Jan 2011
DOIs
Publication statusPublished - Jul 2011

Keywords

  • human colonic bacteria
  • broiler-chickens
  • COA-transferase
  • fatty-acids
  • sp nov.
  • clostridium-propionicum
  • cecal content
  • gen. nov.
  • community
  • gut

Cite this

Eeckhaut, V., Van Immerseel, F., Croubels, S., De Baere, S., Haesebrouck, F., Ducatelle, R., ... Vandamme, P. (2011). Butyrate production in phylogenetically diverse Firmicutes isolated from the chicken caecum. Microbial Biotechnology, 4(4), 503-512. https://doi.org/10.1111/j.1751-7915.2010.00244.x