c-erbB-2 is not a major factor in the development of colorectal cancer

J. A. McKay, Joseph Loane, M. M. Ameyaw, H. L. McLeod, V. G. Ross, Graeme Ian Murray, J. Cassidy

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Abstract

We have investigated c-erbB-2 protein expression in a large cohort of well-characterized colorectal tumours, and in a subset of lymph node metastases. We have also evaluated a Val(655)lle single nucleotide polymorphism, which is associated with an increased risk of breast cancer, in a subset of the colorectal cancer patients and in healthy control subjects. Immunohistochemical studies revealed that while 81.8% of tumours expressed c-erbB-2, in the majority of cases equivalent levels of c-erb-B2 were seen in adjacent normal mucosa. Colon tumours were significantly more likely to express c-erbB-2 than rectal tumours (P=0.015). Only 52.4% of the metastases displayed staining patterns concordant with their primary tumour, indicating that determination of c-erbB-2 protein in colorectal tumours cannot predict the status of lymph node metastases, PCR-RFLP analysis of the Val(655)lle single nucleotide polymorphism demonstrated that allele Frequencies were identical between colorectal cancer patients and a control group of Caucasian subjects (lle=0.80 and Val=0.20 in each case), indicating that it is not related to the risk of developing colorectal cancer in this population. Furthermore, there was no relationship between c-erbB-2 protein expression and gene polymorphism (P=0.58). In terms of prognosis, no association was seen between either c-erbB-2 protein expression or the presence of the Val allele and patient survival (P > 0.05 in each case), suggesting that c-erbB-2 is not a prognostic marker in colorectal cancer. (C) 2002 Cancer Research UK.

Original languageEnglish
Pages (from-to)568-573
Number of pages5
JournalBritish Journal of Cancer
Volume86
Issue number4
DOIs
Publication statusPublished - 2002

Keywords

  • colorectal cancer
  • c-erbB-2
  • immunohistochemistry
  • PCR-RFLP
  • polymorphism
  • prognosis
  • HER2 GENETIC-POLYMORPHISM
  • BREAST-CANCER
  • PROGNOSTIC-SIGNIFICANCE
  • EXPRESSION
  • HER-2/NEU
  • SURVIVAL
  • PROTEIN
  • ADENOCARCINOMA
  • AMPLIFICATION
  • ONCOPROTEIN

Cite this

McKay, J. A., Loane, J., Ameyaw, M. M., McLeod, H. L., Ross, V. G., Murray, G. I., & Cassidy, J. (2002). c-erbB-2 is not a major factor in the development of colorectal cancer. British Journal of Cancer, 86(4), 568-573. https://doi.org/10.1038/sj.bjc.6600127