C-MYC Messenger-RNA in Cytoskeletal-Bound Polysomes in Fibroblasts

J E HESKETH, Gillian Patricia Campbell, P F WHITELAW

    Research output: Contribution to journalArticle

    43 Citations (Scopus)

    Abstract

    3T3 fibroblasts were treated sequentially with 25 mM-KCl/0.05% Nonidet P40, mM-KCl/0.05% Nonidet P40 and finally with 1% Nonidet P40/1% deoxycholate in orde to release free, cytoskeletal-bound and membrane-bound polysomes respectively. The membrane-bound fraction was enriched in the mRNA for the membrane protein beta-2-microglobulin, whereas the cytoskeletal-bound polysomes were enriched in c-myc mRNA. Actin mRNA was present in both free and cytoskeletal-bound polysomes. The results suggest that cytoskeletal-bound polysomes are involved in the translation of specific mRNA species.

    Original languageEnglish
    Pages (from-to)607-609
    Number of pages3
    JournalBiochemical Journal
    Volume274
    Publication statusPublished - 1 Mar 1991

    Keywords

    • protein-synthesis
    • muscle-cells
    • polyribosomes
    • association
    • insulin
    • growth

    Cite this

    HESKETH, J. E., Campbell, G. P., & WHITELAW, P. F. (1991). C-MYC Messenger-RNA in Cytoskeletal-Bound Polysomes in Fibroblasts. Biochemical Journal, 274, 607-609.

    C-MYC Messenger-RNA in Cytoskeletal-Bound Polysomes in Fibroblasts. / HESKETH, J E ; Campbell, Gillian Patricia; WHITELAW, P F .

    In: Biochemical Journal, Vol. 274, 01.03.1991, p. 607-609.

    Research output: Contribution to journalArticle

    HESKETH, JE, Campbell, GP & WHITELAW, PF 1991, 'C-MYC Messenger-RNA in Cytoskeletal-Bound Polysomes in Fibroblasts', Biochemical Journal, vol. 274, pp. 607-609.
    HESKETH JE, Campbell GP, WHITELAW PF. C-MYC Messenger-RNA in Cytoskeletal-Bound Polysomes in Fibroblasts. Biochemical Journal. 1991 Mar 1;274:607-609.
    HESKETH, J E ; Campbell, Gillian Patricia ; WHITELAW, P F . / C-MYC Messenger-RNA in Cytoskeletal-Bound Polysomes in Fibroblasts. In: Biochemical Journal. 1991 ; Vol. 274. pp. 607-609.
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    AB - 3T3 fibroblasts were treated sequentially with 25 mM-KCl/0.05% Nonidet P40, mM-KCl/0.05% Nonidet P40 and finally with 1% Nonidet P40/1% deoxycholate in orde to release free, cytoskeletal-bound and membrane-bound polysomes respectively. The membrane-bound fraction was enriched in the mRNA for the membrane protein beta-2-microglobulin, whereas the cytoskeletal-bound polysomes were enriched in c-myc mRNA. Actin mRNA was present in both free and cytoskeletal-bound polysomes. The results suggest that cytoskeletal-bound polysomes are involved in the translation of specific mRNA species.

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