C-MYC Messenger-RNA in Cytoskeletal-Bound Polysomes in Fibroblasts

J E HESKETH, Gillian Patricia Campbell, P F WHITELAW

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    Abstract

    3T3 fibroblasts were treated sequentially with 25 mM-KCl/0.05% Nonidet P40, mM-KCl/0.05% Nonidet P40 and finally with 1% Nonidet P40/1% deoxycholate in orde to release free, cytoskeletal-bound and membrane-bound polysomes respectively. The membrane-bound fraction was enriched in the mRNA for the membrane protein beta-2-microglobulin, whereas the cytoskeletal-bound polysomes were enriched in c-myc mRNA. Actin mRNA was present in both free and cytoskeletal-bound polysomes. The results suggest that cytoskeletal-bound polysomes are involved in the translation of specific mRNA species.

    Original languageEnglish
    Pages (from-to)607-609
    Number of pages3
    JournalBiochemical Journal
    Volume274
    Publication statusPublished - 1 Mar 1991

    Keywords

    • protein-synthesis
    • muscle-cells
    • polyribosomes
    • association
    • insulin
    • growth

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