C-MYC Messenger-RNA in Cytoskeletal-Bound Polysomes in Fibroblasts

J E  HESKETH; Gillian Patricia Campbell; P F  WHITELAW

C-MYC Messenger-RNA in Cytoskeletal-Bound Polysomes in Fibroblasts

J E HESKETH, Gillian Patricia Campbell, P F WHITELAW

The Rowett Institute of Nutrition and Health

Research output: Contribution to journal › Article › peer-review

43 Citations (Scopus)

Abstract

3T3 fibroblasts were treated sequentially with 25 mM-KCl/0.05% Nonidet P40, mM-KCl/0.05% Nonidet P40 and finally with 1% Nonidet P40/1% deoxycholate in orde to release free, cytoskeletal-bound and membrane-bound polysomes respectively. The membrane-bound fraction was enriched in the mRNA for the membrane protein beta-2-microglobulin, whereas the cytoskeletal-bound polysomes were enriched in c-myc mRNA. Actin mRNA was present in both free and cytoskeletal-bound polysomes. The results suggest that cytoskeletal-bound polysomes are involved in the translation of specific mRNA species.

Original language	English
Pages (from-to)	607-609
Number of pages	3
Journal	Biochemical Journal
Volume	274
Publication status	Published - 1 Mar 1991

Keywords

protein-synthesis
muscle-cells
polyribosomes
association
insulin
growth

Cite this

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title = "C-MYC Messenger-RNA in Cytoskeletal-Bound Polysomes in Fibroblasts",

abstract = "3T3 fibroblasts were treated sequentially with 25 mM-KCl/0.05% Nonidet P40, mM-KCl/0.05% Nonidet P40 and finally with 1% Nonidet P40/1% deoxycholate in orde to release free, cytoskeletal-bound and membrane-bound polysomes respectively. The membrane-bound fraction was enriched in the mRNA for the membrane protein beta-2-microglobulin, whereas the cytoskeletal-bound polysomes were enriched in c-myc mRNA. Actin mRNA was present in both free and cytoskeletal-bound polysomes. The results suggest that cytoskeletal-bound polysomes are involved in the translation of specific mRNA species.",

keywords = "protein-synthesis, muscle-cells, polyribosomes, association, insulin, growth",

author = "HESKETH, {J E} and Campbell, {Gillian Patricia} and WHITELAW, {P F}",

year = "1991",

month = mar,

day = "1",

language = "English",

volume = "274",

pages = "607--609",

journal = "Biochemical Journal",

issn = "0264-6021",

publisher = "Portland Press Ltd.",

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TY - JOUR

T1 - C-MYC Messenger-RNA in Cytoskeletal-Bound Polysomes in Fibroblasts

AU - HESKETH, J E

AU - Campbell, Gillian Patricia

AU - WHITELAW, P F

PY - 1991/3/1

Y1 - 1991/3/1

N2 - 3T3 fibroblasts were treated sequentially with 25 mM-KCl/0.05% Nonidet P40, mM-KCl/0.05% Nonidet P40 and finally with 1% Nonidet P40/1% deoxycholate in orde to release free, cytoskeletal-bound and membrane-bound polysomes respectively. The membrane-bound fraction was enriched in the mRNA for the membrane protein beta-2-microglobulin, whereas the cytoskeletal-bound polysomes were enriched in c-myc mRNA. Actin mRNA was present in both free and cytoskeletal-bound polysomes. The results suggest that cytoskeletal-bound polysomes are involved in the translation of specific mRNA species.

AB - 3T3 fibroblasts were treated sequentially with 25 mM-KCl/0.05% Nonidet P40, mM-KCl/0.05% Nonidet P40 and finally with 1% Nonidet P40/1% deoxycholate in orde to release free, cytoskeletal-bound and membrane-bound polysomes respectively. The membrane-bound fraction was enriched in the mRNA for the membrane protein beta-2-microglobulin, whereas the cytoskeletal-bound polysomes were enriched in c-myc mRNA. Actin mRNA was present in both free and cytoskeletal-bound polysomes. The results suggest that cytoskeletal-bound polysomes are involved in the translation of specific mRNA species.

KW - protein-synthesis

KW - muscle-cells

KW - polyribosomes

KW - association

KW - insulin

KW - growth

M3 - Article

SN - 0264-6021

VL - 274

SP - 607

EP - 609

JO - Biochemical Journal

JF - Biochemical Journal

ER -

C-MYC Messenger-RNA in Cytoskeletal-Bound Polysomes in Fibroblasts

Abstract

Keywords

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