Calpastatin-modulation of m-calpain activity is required for myoblast fusion

Denis Pierre Balcerzak, P Cottin, S Poussard, A Cucuron, J J Brustis, A Ducastaing

    Research output: Contribution to journalArticle

    41 Citations (Scopus)

    Abstract

    Previous studies have demonstrated a role for m-calpain in myoblast fusion. Moreover, the presence, in differentiated cells, of a highly specific endogenous inhibitor of calpain, calpastatin, has led to the hypothesis that a regulation of or a protection against m-calpain activity by calpastatin could also occur during the earlier stages of muscle cell differentiation. In order to verify this hypothesis, we have investigated, in myoblast culture, the appearance of calpastatin-mRNA and its corresponding protein. Our results provide evidence that calpastatin is already present at the earlier stages of myoblast differentiation and that a significant decrease of the levels of calpastatin mRNA and its protein precedes myoblast fusion. In addition, the induction of an artificial decrease in calpastatin level, via an appropriate antisense oligodeoxyribonucleotide methodology, leads to earlier and faster myoblast fusion. Together with previous studies, these results indicate that m-calpain and calpastatin are functionally involved in myoblast fusion. Our findings also demonstrate that an acute "hyperactivity" of m-calpain resulting from the decrease of calpastatin synthesis is necessary during the early stages of this step of differentiation.

    Original languageEnglish
    Pages (from-to)247-253
    Number of pages7
    JournalEuropean Journal of Cell Biology
    Volume75
    Issue number3
    Publication statusPublished - Mar 1998

    Keywords

    • Antisense oligodeoxyribonucleotides
    • calpain
    • calpastatin
    • culture in vitro
    • myoblast fusion
    • MUSCLE-CELLS
    • MYOGENIC DIFFERENTIATION
    • MOLECULAR DIVERSITY
    • INHIBITOR
    • PROTEASE
    • ERYTHROCYTES
    • FUSIBILITY
    • PROTEINS
    • CLEAVAGE
    • SYSTEM

    Cite this

    Balcerzak, D. P., Cottin, P., Poussard, S., Cucuron, A., Brustis, J. J., & Ducastaing, A. (1998). Calpastatin-modulation of m-calpain activity is required for myoblast fusion. European Journal of Cell Biology, 75(3), 247-253.