Elevation of intracellular cAMP levels can increase axon regeneration both in vivo and in vitro and manipulation of cAMP signalling can also alter growth cone responses to guidance cues. The precise mechanisms involved in cAMP mediated axon growth and guidance remain to be fully determined. Exchange protein activated by cAMP (Epac) is the only identified downstream effecter of cAMP other than protein kinase A (PKA). Using both rat and Xenopus model systems we have performed neurite outgrowth and growth cone turning assays to assess a possible role for Epac in mediating axon growth, guidance and regeneration. Given that cAMP levels are developmentally regulated, we have used immunohistochemistry and western blotting to also study the developmental regulation of Epac expression. These studies may further dissect the nature of cAMP-mediated axon growth and guidance and provide more effective targets for promoting axon regeneration in vivo.
|Number of pages||1|
|Publication status||Published - 2005|
|Event||British Neuroscience Association Meeting - Brighton, United Kingdom|
Duration: 3 Apr 2005 → 6 Apr 2005
|Conference||British Neuroscience Association Meeting|
|Period||3/04/05 → 6/04/05|