Campylobacter fetus Subspecies Contain Conserved Type IV Secretion Systems on Multiple Genomic Islands and Plasmids

Linda van der Graaf-van Bloois, William G. Miller, Emma Yee, Gregor Gorkiewicz, Ken J. Forbes, Aldert L. Zomer, Jaap A. Wagenaar, Birgitta Duim

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Abstract

The features contributing to differences in pathogenicity of the Campylobacter fetus subspecies are unknown. Putative factors involved in pathogenesis are located in genomic islands that encode a type IV secretion system (T4SS) and fic domain (filamentation induced by cyclic AMP) proteins, which may disrupt host cell processes. In the genomes of 27 C. fetus strains, three phylogenetically-different T4SS-encoding regions (T4SSs) were identified: one was located in both the chromosome and in extra-chromosomal plasmids; one was located exclusively in the chromosome; and one exclusively in extra-chromosomal plasmids. We observed that C. fetus strains can contain multiple T4SSs and that homologous T4SSs can be present both in chromosomal genomic islands (GI) and on plasmids in the C. fetus strains. The GIs of the chromosomally located T4SS differed mainly by the presence of fic genes, insertion sequence elements and phage-related or hypothetical proteins. Comparative analysis showed that T4SS sequences, inserted in the same locations, were conserved in the studied C. fetus genomes. Using phylogenetic analysis of the T4SSs, it was shown that C. fetus may have acquired the T4SS regions from other Campylobacter species by horizontal gene transfer. The identified T4SSs and fic genes were found in Cff and Cfv strains, although the presence of T4SSs and fic genes were significantly associated with Cfv strains. The T4SSs and fic genes could not be associated with S-layer serotypes or geographical origin of the strains.

Original languageEnglish
Article numbere0152832
Pages (from-to)1-15
Number of pages15
JournalPloS ONE
Volume11
Issue number4
DOIs
Publication statusPublished - 6 Apr 2016

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Campylobacter fetus
genomic islands
Genomic Islands
plasmids
Plasmids
Genes
Chromosomes
genes
Genome
chromosomes
Gene transfer
Horizontal Gene Transfer
Bacteriophages
DNA Transposable Elements
Campylobacter
genome
Insertional Mutagenesis
cyclic AMP
transposons
bacteriophages

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Graaf-van Bloois, L. V. D., Miller, W. G., Yee, E., Gorkiewicz, G., Forbes, K. J., Zomer, A. L., ... Duim, B. (2016). Campylobacter fetus Subspecies Contain Conserved Type IV Secretion Systems on Multiple Genomic Islands and Plasmids. PloS ONE, 11(4), 1-15. [e0152832]. https://doi.org/10.1371/journal.pone.0152832

Campylobacter fetus Subspecies Contain Conserved Type IV Secretion Systems on Multiple Genomic Islands and Plasmids. / Graaf-van Bloois, Linda van der; Miller, William G.; Yee, Emma; Gorkiewicz, Gregor; Forbes, Ken J.; Zomer, Aldert L.; Wagenaar, Jaap A.; Duim, Birgitta.

In: PloS ONE, Vol. 11, No. 4, e0152832, 06.04.2016, p. 1-15.

Research output: Contribution to journalArticle

Graaf-van Bloois, LVD, Miller, WG, Yee, E, Gorkiewicz, G, Forbes, KJ, Zomer, AL, Wagenaar, JA & Duim, B 2016, 'Campylobacter fetus Subspecies Contain Conserved Type IV Secretion Systems on Multiple Genomic Islands and Plasmids', PloS ONE, vol. 11, no. 4, e0152832, pp. 1-15. https://doi.org/10.1371/journal.pone.0152832
Graaf-van Bloois LVD, Miller WG, Yee E, Gorkiewicz G, Forbes KJ, Zomer AL et al. Campylobacter fetus Subspecies Contain Conserved Type IV Secretion Systems on Multiple Genomic Islands and Plasmids. PloS ONE. 2016 Apr 6;11(4):1-15. e0152832. https://doi.org/10.1371/journal.pone.0152832
Graaf-van Bloois, Linda van der ; Miller, William G. ; Yee, Emma ; Gorkiewicz, Gregor ; Forbes, Ken J. ; Zomer, Aldert L. ; Wagenaar, Jaap A. ; Duim, Birgitta. / Campylobacter fetus Subspecies Contain Conserved Type IV Secretion Systems on Multiple Genomic Islands and Plasmids. In: PloS ONE. 2016 ; Vol. 11, No. 4. pp. 1-15.
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abstract = "The features contributing to differences in pathogenicity of the Campylobacter fetus subspecies are unknown. Putative factors involved in pathogenesis are located in genomic islands that encode a type IV secretion system (T4SS) and fic domain (filamentation induced by cyclic AMP) proteins, which may disrupt host cell processes. In the genomes of 27 C. fetus strains, three phylogenetically-different T4SS-encoding regions (T4SSs) were identified: one was located in both the chromosome and in extra-chromosomal plasmids; one was located exclusively in the chromosome; and one exclusively in extra-chromosomal plasmids. We observed that C. fetus strains can contain multiple T4SSs and that homologous T4SSs can be present both in chromosomal genomic islands (GI) and on plasmids in the C. fetus strains. The GIs of the chromosomally located T4SS differed mainly by the presence of fic genes, insertion sequence elements and phage-related or hypothetical proteins. Comparative analysis showed that T4SS sequences, inserted in the same locations, were conserved in the studied C. fetus genomes. Using phylogenetic analysis of the T4SSs, it was shown that C. fetus may have acquired the T4SS regions from other Campylobacter species by horizontal gene transfer. The identified T4SSs and fic genes were found in Cff and Cfv strains, although the presence of T4SSs and fic genes were significantly associated with Cfv strains. The T4SSs and fic genes could not be associated with S-layer serotypes or geographical origin of the strains.",
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note = "Acknowledgments We like to thank Dr. John Devenish and Dr. Brian Brooks (Canadian Food Inspection Agency) for providing strains. We thank Nathaniel Simon and Mary Chapman for the generation of Illumina MiSeq reads and we thank James Bono for the generation of PacBio RS reads. Funding: The authors have no support or funding to report.",
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N1 - Acknowledgments We like to thank Dr. John Devenish and Dr. Brian Brooks (Canadian Food Inspection Agency) for providing strains. We thank Nathaniel Simon and Mary Chapman for the generation of Illumina MiSeq reads and we thank James Bono for the generation of PacBio RS reads. Funding: The authors have no support or funding to report.

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