Abstract
The human fungal pathogen Candida albicans undergoes reversible morphogenetic transitions between yeast, hyphal and pseudohyphal forms. The fungal vacuole actively participates in differentiation processes and plays a key role supporting hyphal growth. The ABG1 gene of C. albicans encodes an essential protein located in the vacuolar membranes of both yeast and hyphae. Using fluorescence microscopy of a green fluorescent protein-tagged version of Abg1p, a fraction of the protein was detected in hyphal tips, not associated with vacuolar membranes. Live cell imaging of emerging germ tubes showed that Abg1p migrated to the polarized growth site and colocalized with endocytic vesicles. Phenotypic analysis of a methionine-regulated conditional mutant confirmed that Abg1p is involved in endocytosis.
| Original language | English |
|---|---|
| Pages (from-to) | 293-300 |
| Number of pages | 8 |
| Journal | FEMS Yeast Research |
| Volume | 9 |
| Issue number | 2 |
| Early online date | 21 Jan 2009 |
| DOIs | |
| Publication status | Published - Mar 2009 |
Keywords
- artificial gene fusion
- Candida albicans
- endocytosis
- fungal proteins
- genes, reporter
- green fluorescent proteins
- humans
- hyphae
- microscopy, fluorescence
- recombinant fusion proteins
- ABG1 gene
- spitzenkörper
- vacuole