Candida albicans ABG1 gene is involved in endocytosis

Veronica Veses, Manuel Casanova, Amelia Murgui, Neil A R Gow, José P Martínez

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6 Citations (Scopus)

Abstract

The human fungal pathogen Candida albicans undergoes reversible morphogenetic transitions between yeast, hyphal and pseudohyphal forms. The fungal vacuole actively participates in differentiation processes and plays a key role supporting hyphal growth. The ABG1 gene of C. albicans encodes an essential protein located in the vacuolar membranes of both yeast and hyphae. Using fluorescence microscopy of a green fluorescent protein-tagged version of Abg1p, a fraction of the protein was detected in hyphal tips, not associated with vacuolar membranes. Live cell imaging of emerging germ tubes showed that Abg1p migrated to the polarized growth site and colocalized with endocytic vesicles. Phenotypic analysis of a methionine-regulated conditional mutant confirmed that Abg1p is involved in endocytosis.
Original languageEnglish
Pages (from-to)293-300
Number of pages8
JournalFEMS Yeast Research
Volume9
Issue number2
Early online date21 Jan 2009
DOIs
Publication statusPublished - Mar 2009

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Keywords

  • artificial gene fusion
  • Candida albicans
  • endocytosis
  • fungal proteins
  • genes, reporter
  • green fluorescent proteins
  • humans
  • hyphae
  • microscopy, fluorescence
  • recombinant fusion proteins
  • ABG1 gene
  • spitzenkörper
  • vacuole

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