Candida albicans scavenges host zinc via Pra1 during endothelial invasion

Francesco Citiulo, Ilse D Jacobsen, Pedro Miramón, Lydia Schild, Sascha Brunke, Peter Zipfel, Matthias Brock, Bernhard Hube, Duncan Wilson

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Abstract

The ability of pathogenic microorganisms to assimilate essential nutrients from their hosts is critical for pathogenesis. Here we report endothelial zinc sequestration by the major human fungal pathogen, Candida albicans. We hypothesised that, analogous to siderophore-mediated iron acquisition, C. albicans utilises an extracellular zinc scavenger for acquiring this essential metal. We postulated that such a "zincophore" system would consist of a secreted factor with zinc-binding properties, which can specifically reassociate with the fungal cell surface. In silico analysis of the C. albicans secretome for proteins with zinc binding motifs identified the pH-regulated antigen 1 (Pra1). Three-dimensional modelling of Pra1 indicated the presence of at least two zinc coordination sites. Indeed, recombinantly expressed Pra1 exhibited zinc binding properties in vitro. Deletion of PRA1 in C. albicans prevented fungal sequestration and utilisation of host zinc, and specifically blocked host cell damage in the absence of exogenous zinc. Phylogenetic analysis revealed that PRA1 arose in an ancient fungal lineage and developed synteny with ZRT1 (encoding a zinc transporter) before divergence of the Ascomycota and Basidiomycota. Structural modelling indicated physical interaction between Pra1 and Zrt1 and we confirmed this experimentally by demonstrating that Zrt1 was essential for binding of soluble Pra1 to the cell surface of C. albicans. Therefore, we have identified a novel metal acquisition system consisting of a secreted zinc scavenger ("zincophore"), which reassociates with the fungal cell. Furthermore, functional similarities with phylogenetically unrelated prokaryotic systems indicate that syntenic zinc acquisition loci have been independently selected during evolution.
Original languageEnglish
Article numbere1002777
Number of pages14
JournalPLoS Pathogens
Volume8
Issue number6
DOIs
Publication statusPublished - 28 Jun 2012

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Candida albicans
Zinc
Antigens
Metals
Synteny
Siderophores
Basidiomycota
Ascomycota
Computer Simulation
Iron
Food

Keywords

  • amino acid sequence
  • biological evolution
  • blotting, Western
  • Candida albicans
  • candidiasis
  • cation transport proteins
  • cells, cultured
  • endothelial cells
  • fungal proteins
  • host-parasite interactions
  • humans
  • molecular sequence data
  • phylogeny
  • protein conformation
  • Saccharomyces cerevisiae Proteins
  • umbilical veins
  • zinc

Cite this

Citiulo, F., Jacobsen, I. D., Miramón, P., Schild, L., Brunke, S., Zipfel, P., ... Wilson, D. (2012). Candida albicans scavenges host zinc via Pra1 during endothelial invasion. PLoS Pathogens, 8(6), [e1002777]. https://doi.org/10.1371/journal.ppat.1002777

Candida albicans scavenges host zinc via Pra1 during endothelial invasion. / Citiulo, Francesco; Jacobsen, Ilse D; Miramón, Pedro; Schild, Lydia; Brunke, Sascha; Zipfel, Peter; Brock, Matthias; Hube, Bernhard; Wilson, Duncan.

In: PLoS Pathogens, Vol. 8, No. 6, e1002777, 28.06.2012.

Research output: Contribution to journalArticle

Citiulo, F, Jacobsen, ID, Miramón, P, Schild, L, Brunke, S, Zipfel, P, Brock, M, Hube, B & Wilson, D 2012, 'Candida albicans scavenges host zinc via Pra1 during endothelial invasion', PLoS Pathogens, vol. 8, no. 6, e1002777. https://doi.org/10.1371/journal.ppat.1002777
Citiulo F, Jacobsen ID, Miramón P, Schild L, Brunke S, Zipfel P et al. Candida albicans scavenges host zinc via Pra1 during endothelial invasion. PLoS Pathogens. 2012 Jun 28;8(6). e1002777. https://doi.org/10.1371/journal.ppat.1002777
Citiulo, Francesco ; Jacobsen, Ilse D ; Miramón, Pedro ; Schild, Lydia ; Brunke, Sascha ; Zipfel, Peter ; Brock, Matthias ; Hube, Bernhard ; Wilson, Duncan. / Candida albicans scavenges host zinc via Pra1 during endothelial invasion. In: PLoS Pathogens. 2012 ; Vol. 8, No. 6.
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