Cannabidiol, a safe and non-psychotropic ingredient of the marijuana plant Cannabis sativa, is protective in a murine model of colitis

Francesca Borrelli, Gabriella Aviello, Barbara Romano, Pierangelo Orlando, Raffaele Capasso, Francesco Maiello, Federico Guadagno, Stefania Petrosino, Francesco Capasso, Vincenzo Di Marzo, Angelo A Izzo

Research output: Contribution to journalArticle

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Abstract

Inflammatory bowel disease affects millions of individuals; nevertheless, pharmacological treatment is disappointingly unsatisfactory. Cannabidiol, a safe and non-psychotropic ingredient of marijuana, exerts pharmacological effects (e.g., antioxidant) and mechanisms (e.g., inhibition of endocannabinoids enzymatic degradation) potentially beneficial for the inflamed gut. Thus, we investigated the effect of cannabidiol in a murine model of colitis. Colitis was induced in mice by intracolonic administration of dinitrobenzene sulfonic acid. Inflammation was assessed both macroscopically and histologically. In the inflamed colon, cyclooxygenase-2 and inducible nitric oxide synthase (iNOS) were evaluated by Western blot, interleukin-1beta and interleukin-10 by ELISA, and endocannabinoids by isotope dilution liquid chromatography-mass spectrometry. Human colon adenocarcinoma (Caco-2) cells were used to evaluate the effect of cannabidiol on oxidative stress. Cannabidiol reduced colon injury, inducible iNOS (but not cyclooxygenase-2) expression, and interleukin-1beta, interleukin-10, and endocannabinoid changes associated with 2,4,6-dinitrobenzene sulfonic acid administration. In Caco-2 cells, cannabidiol reduced reactive oxygen species production and lipid peroxidation. In conclusion, cannabidiol, a likely safe compound, prevents experimental colitis in mice.

Original languageEnglish
Pages (from-to)1111-1121
Number of pages11
JournalJournal of Molecular Medicine
Volume87
Issue number11
DOIs
Publication statusPublished - Nov 2009

Fingerprint

Cannabidiol
Colitis
Cannabis
Endocannabinoids
Colon
Caco-2 Cells
Sulfonic Acids
Nitric Oxide Synthase Type II
Cyclooxygenase 2
Interleukin-1beta
Interleukin-10
Dinitrobenzenes
Pharmacology
Inflammatory Bowel Diseases
Liquid Chromatography
Isotopes
Lipid Peroxidation
Reactive Oxygen Species
Mass Spectrometry
Adenocarcinoma

Keywords

  • Amidohydrolases
  • Animals
  • Antioxidants
  • Caco-2 Cells
  • Cannabidiol
  • Cannabinoid Receptor Modulators
  • Cannabis
  • Cell Survival
  • Colitis
  • Colon
  • Cyclooxygenase 2
  • Gene Expression
  • Humans
  • Interleukin-10
  • Interleukin-1beta
  • Male
  • Mice
  • Mice, Inbred ICR
  • Nitric Oxide Synthase Type II
  • Organ Size
  • Journal Article

Cite this

Cannabidiol, a safe and non-psychotropic ingredient of the marijuana plant Cannabis sativa, is protective in a murine model of colitis. / Borrelli, Francesca; Aviello, Gabriella; Romano, Barbara; Orlando, Pierangelo; Capasso, Raffaele; Maiello, Francesco; Guadagno, Federico; Petrosino, Stefania; Capasso, Francesco; Di Marzo, Vincenzo; Izzo, Angelo A.

In: Journal of Molecular Medicine, Vol. 87, No. 11, 11.2009, p. 1111-1121.

Research output: Contribution to journalArticle

Borrelli, F, Aviello, G, Romano, B, Orlando, P, Capasso, R, Maiello, F, Guadagno, F, Petrosino, S, Capasso, F, Di Marzo, V & Izzo, AA 2009, 'Cannabidiol, a safe and non-psychotropic ingredient of the marijuana plant Cannabis sativa, is protective in a murine model of colitis', Journal of Molecular Medicine, vol. 87, no. 11, pp. 1111-1121. https://doi.org/10.1007/s00109-009-0512-x
Borrelli, Francesca ; Aviello, Gabriella ; Romano, Barbara ; Orlando, Pierangelo ; Capasso, Raffaele ; Maiello, Francesco ; Guadagno, Federico ; Petrosino, Stefania ; Capasso, Francesco ; Di Marzo, Vincenzo ; Izzo, Angelo A. / Cannabidiol, a safe and non-psychotropic ingredient of the marijuana plant Cannabis sativa, is protective in a murine model of colitis. In: Journal of Molecular Medicine. 2009 ; Vol. 87, No. 11. pp. 1111-1121.
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abstract = "Inflammatory bowel disease affects millions of individuals; nevertheless, pharmacological treatment is disappointingly unsatisfactory. Cannabidiol, a safe and non-psychotropic ingredient of marijuana, exerts pharmacological effects (e.g., antioxidant) and mechanisms (e.g., inhibition of endocannabinoids enzymatic degradation) potentially beneficial for the inflamed gut. Thus, we investigated the effect of cannabidiol in a murine model of colitis. Colitis was induced in mice by intracolonic administration of dinitrobenzene sulfonic acid. Inflammation was assessed both macroscopically and histologically. In the inflamed colon, cyclooxygenase-2 and inducible nitric oxide synthase (iNOS) were evaluated by Western blot, interleukin-1beta and interleukin-10 by ELISA, and endocannabinoids by isotope dilution liquid chromatography-mass spectrometry. Human colon adenocarcinoma (Caco-2) cells were used to evaluate the effect of cannabidiol on oxidative stress. Cannabidiol reduced colon injury, inducible iNOS (but not cyclooxygenase-2) expression, and interleukin-1beta, interleukin-10, and endocannabinoid changes associated with 2,4,6-dinitrobenzene sulfonic acid administration. In Caco-2 cells, cannabidiol reduced reactive oxygen species production and lipid peroxidation. In conclusion, cannabidiol, a likely safe compound, prevents experimental colitis in mice.",
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AU - Orlando, Pierangelo

AU - Capasso, Raffaele

AU - Maiello, Francesco

AU - Guadagno, Federico

AU - Petrosino, Stefania

AU - Capasso, Francesco

AU - Di Marzo, Vincenzo

AU - Izzo, Angelo A

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AB - Inflammatory bowel disease affects millions of individuals; nevertheless, pharmacological treatment is disappointingly unsatisfactory. Cannabidiol, a safe and non-psychotropic ingredient of marijuana, exerts pharmacological effects (e.g., antioxidant) and mechanisms (e.g., inhibition of endocannabinoids enzymatic degradation) potentially beneficial for the inflamed gut. Thus, we investigated the effect of cannabidiol in a murine model of colitis. Colitis was induced in mice by intracolonic administration of dinitrobenzene sulfonic acid. Inflammation was assessed both macroscopically and histologically. In the inflamed colon, cyclooxygenase-2 and inducible nitric oxide synthase (iNOS) were evaluated by Western blot, interleukin-1beta and interleukin-10 by ELISA, and endocannabinoids by isotope dilution liquid chromatography-mass spectrometry. Human colon adenocarcinoma (Caco-2) cells were used to evaluate the effect of cannabidiol on oxidative stress. Cannabidiol reduced colon injury, inducible iNOS (but not cyclooxygenase-2) expression, and interleukin-1beta, interleukin-10, and endocannabinoid changes associated with 2,4,6-dinitrobenzene sulfonic acid administration. In Caco-2 cells, cannabidiol reduced reactive oxygen species production and lipid peroxidation. In conclusion, cannabidiol, a likely safe compound, prevents experimental colitis in mice.

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