Cannabinoid CB1 receptor elevation of intracellular calcium in neuroblastoma SH-SY5Y cells

interactions with muscarinic and delta-opioid receptors

Pietro Marini, Aniello Schiano Moriello, Luigia Cristino, Maura Palmery, Luciano De Petrocellis, Vincenzo Di Marzo

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Although coupled to Gi/o proteins, cannabinoid CB1 receptors can also activate intracellular Ca2+ ([Ca2+]i) accumulation through not fully understood mechanisms. We report that in, human neuroblastoma SH-SY5Y cells, CB1 activation with the specific agonist arachidonoylchloroethanolamide (ACEA), weakly elevates [Ca2+]i and that this effect, when using low (1–100 nM) concentrations of ACEA, is enhanced by the previous activation of Gq/11-coupled M3 muscarinic receptors with carbachol, dose-dependently and up to ~ 8-fold. A similar behaviour was also observed with carbachol and the Gi/o-coupled d-opioid receptor. Furthermore, stimulation of CB1 receptors produced a concentration-dependent leftward shift of the elevation of [Ca2+]i by d-opioid receptors. These stimulatory effects were variedly attenuated by selective antagonists of each receptor, pertussis toxin, inhibitors of phospholipase C (U73122 and D609), and, when assessed in the presence of extracellular Ca2+, by the block of voltage-activated calcium channels. Cholera toxin only slightly inhibited the Gq/11-Gi/o-mediated cross-talk, but induced a stronger inhibition of the Gi/o-Gi/o-mediated interaction. These findings suggest that activation of M3 muscarinic receptors might produce a qualitative alteration of the signaling associated with Gi/o-coupled receptors, and that sequential activation of CB1 and d-opioid receptors, both coupled to Gi/o, produces instead synergistic effects on [Ca2+]i elevation.
Original languageEnglish
Pages (from-to)1289-1303
Number of pages15
JournalBiochimica et Biophysica Acta. Molecular Cell Research
Volume1793
Issue number7
Early online date13 May 2009
DOIs
Publication statusPublished - Jul 2009

Fingerprint

Cannabinoid Receptor CB1
delta Opioid Receptor
Opioid Receptors
Neuroblastoma
Muscarinic M3 Receptors
Cell Communication
Cholinergic Agents
Carbachol
Calcium
Cholera Toxin
Type C Phospholipases
Calcium Channels
Proteins

Keywords

  • calcium
  • cannabinoid
  • opioid
  • acetycholine
  • M3 muscarinic receptor
  • G-protein
  • receptor
  • signaling
  • lipid

Cite this

Cannabinoid CB1 receptor elevation of intracellular calcium in neuroblastoma SH-SY5Y cells : interactions with muscarinic and delta-opioid receptors. / Marini, Pietro; Moriello, Aniello Schiano; Cristino, Luigia; Palmery, Maura; De Petrocellis, Luciano; Di Marzo, Vincenzo.

In: Biochimica et Biophysica Acta. Molecular Cell Research, Vol. 1793, No. 7, 07.2009, p. 1289-1303.

Research output: Contribution to journalArticle

Marini, Pietro ; Moriello, Aniello Schiano ; Cristino, Luigia ; Palmery, Maura ; De Petrocellis, Luciano ; Di Marzo, Vincenzo. / Cannabinoid CB1 receptor elevation of intracellular calcium in neuroblastoma SH-SY5Y cells : interactions with muscarinic and delta-opioid receptors. In: Biochimica et Biophysica Acta. Molecular Cell Research. 2009 ; Vol. 1793, No. 7. pp. 1289-1303.
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AU - Cristino, Luigia

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AB - Although coupled to Gi/o proteins, cannabinoid CB1 receptors can also activate intracellular Ca2+ ([Ca2+]i) accumulation through not fully understood mechanisms. We report that in, human neuroblastoma SH-SY5Y cells, CB1 activation with the specific agonist arachidonoylchloroethanolamide (ACEA), weakly elevates [Ca2+]i and that this effect, when using low (1–100 nM) concentrations of ACEA, is enhanced by the previous activation of Gq/11-coupled M3 muscarinic receptors with carbachol, dose-dependently and up to ~ 8-fold. A similar behaviour was also observed with carbachol and the Gi/o-coupled d-opioid receptor. Furthermore, stimulation of CB1 receptors produced a concentration-dependent leftward shift of the elevation of [Ca2+]i by d-opioid receptors. These stimulatory effects were variedly attenuated by selective antagonists of each receptor, pertussis toxin, inhibitors of phospholipase C (U73122 and D609), and, when assessed in the presence of extracellular Ca2+, by the block of voltage-activated calcium channels. Cholera toxin only slightly inhibited the Gq/11-Gi/o-mediated cross-talk, but induced a stronger inhibition of the Gi/o-Gi/o-mediated interaction. These findings suggest that activation of M3 muscarinic receptors might produce a qualitative alteration of the signaling associated with Gi/o-coupled receptors, and that sequential activation of CB1 and d-opioid receptors, both coupled to Gi/o, produces instead synergistic effects on [Ca2+]i elevation.

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KW - acetycholine

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KW - receptor

KW - signaling

KW - lipid

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