Carbachol stimulates Cl- secretion via activation of two distinct apical Cl- pathways in cultured human T84 intestinal epithelial monolayers

G T McEwan, B H Hirst, N L Simmons

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The mode of action of carbachol in stimulation of transepithelial Cl- secretion in intact human intestinal T84 epithelial monolayers has been investigated in order to determine whether a DIDS-insensitive exit pathway (via CFTR) coexists with a DIDS-sensitive exit pathway at the apical membrane. Carbachol stimulates a transient inward Isc due to Cl- secretion whose magnitude is related to the basal level of inward Isc. The inward current responses to both carbachol and hypo-osmotic media are abolished in nominally Ca(2+)-free media. The action of apical DIDS (100 microM) upon carbachol-stimulated Isc depends on the initial value of the basal Isc. At basal Isc levels 10 microA cm-2, the carbachol stimulation of inward Isc was largely insensitive to 100 microM DIDS. The data are consistent with the participation of both DIDS-sensitive and DIDS insensitive pathways for Cl- at the apical membrane of human intestinal T84 epithelial cells.
Original languageEnglish
Pages (from-to)241-7
Number of pages7
JournalBiochimica et Biophysica Acta
Volume1220
Issue number3
Publication statusPublished - 17 Feb 1994

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4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
Carbachol
Membranes
Epithelial Cells

Keywords

  • 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
  • Calcium
  • Carbachol
  • Cell Line
  • Chlorides
  • Epithelium
  • Humans
  • Hypotonic Solutions
  • Intestines
  • Kinetics
  • Membrane Potentials
  • Time Factors
  • Vasoactive Intestinal Peptide

Cite this

Carbachol stimulates Cl- secretion via activation of two distinct apical Cl- pathways in cultured human T84 intestinal epithelial monolayers. / McEwan, G T; Hirst, B H; Simmons, N L.

In: Biochimica et Biophysica Acta, Vol. 1220, No. 3, 17.02.1994, p. 241-7.

Research output: Contribution to journalArticle

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abstract = "The mode of action of carbachol in stimulation of transepithelial Cl- secretion in intact human intestinal T84 epithelial monolayers has been investigated in order to determine whether a DIDS-insensitive exit pathway (via CFTR) coexists with a DIDS-sensitive exit pathway at the apical membrane. Carbachol stimulates a transient inward Isc due to Cl- secretion whose magnitude is related to the basal level of inward Isc. The inward current responses to both carbachol and hypo-osmotic media are abolished in nominally Ca(2+)-free media. The action of apical DIDS (100 microM) upon carbachol-stimulated Isc depends on the initial value of the basal Isc. At basal Isc levels 10 microA cm-2, the carbachol stimulation of inward Isc was largely insensitive to 100 microM DIDS. The data are consistent with the participation of both DIDS-sensitive and DIDS insensitive pathways for Cl- at the apical membrane of human intestinal T84 epithelial cells.",
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T1 - Carbachol stimulates Cl- secretion via activation of two distinct apical Cl- pathways in cultured human T84 intestinal epithelial monolayers

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AU - Hirst, B H

AU - Simmons, N L

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N2 - The mode of action of carbachol in stimulation of transepithelial Cl- secretion in intact human intestinal T84 epithelial monolayers has been investigated in order to determine whether a DIDS-insensitive exit pathway (via CFTR) coexists with a DIDS-sensitive exit pathway at the apical membrane. Carbachol stimulates a transient inward Isc due to Cl- secretion whose magnitude is related to the basal level of inward Isc. The inward current responses to both carbachol and hypo-osmotic media are abolished in nominally Ca(2+)-free media. The action of apical DIDS (100 microM) upon carbachol-stimulated Isc depends on the initial value of the basal Isc. At basal Isc levels 10 microA cm-2, the carbachol stimulation of inward Isc was largely insensitive to 100 microM DIDS. The data are consistent with the participation of both DIDS-sensitive and DIDS insensitive pathways for Cl- at the apical membrane of human intestinal T84 epithelial cells.

AB - The mode of action of carbachol in stimulation of transepithelial Cl- secretion in intact human intestinal T84 epithelial monolayers has been investigated in order to determine whether a DIDS-insensitive exit pathway (via CFTR) coexists with a DIDS-sensitive exit pathway at the apical membrane. Carbachol stimulates a transient inward Isc due to Cl- secretion whose magnitude is related to the basal level of inward Isc. The inward current responses to both carbachol and hypo-osmotic media are abolished in nominally Ca(2+)-free media. The action of apical DIDS (100 microM) upon carbachol-stimulated Isc depends on the initial value of the basal Isc. At basal Isc levels 10 microA cm-2, the carbachol stimulation of inward Isc was largely insensitive to 100 microM DIDS. The data are consistent with the participation of both DIDS-sensitive and DIDS insensitive pathways for Cl- at the apical membrane of human intestinal T84 epithelial cells.

KW - 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid

KW - Calcium

KW - Carbachol

KW - Cell Line

KW - Chlorides

KW - Epithelium

KW - Humans

KW - Hypotonic Solutions

KW - Intestines

KW - Kinetics

KW - Membrane Potentials

KW - Time Factors

KW - Vasoactive Intestinal Peptide

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JO - Biochimica et Biophysica Acta

JF - Biochimica et Biophysica Acta

SN - 0006-3002

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