The mode of action of carbachol in stimulation of transepithelial Cl- secretion in intact human intestinal T84 epithelial monolayers has been investigated in order to determine whether a DIDS-insensitive exit pathway (via CFTR) coexists with a DIDS-sensitive exit pathway at the apical membrane. Carbachol stimulates a transient inward Isc due to Cl- secretion whose magnitude is related to the basal level of inward Isc. The inward current responses to both carbachol and hypo-osmotic media are abolished in nominally Ca(2+)-free media. The action of apical DIDS (100 microM) upon carbachol-stimulated Isc depends on the initial value of the basal Isc. At basal Isc levels 10 microA cm-2, the carbachol stimulation of inward Isc was largely insensitive to 100 microM DIDS. The data are consistent with the participation of both DIDS-sensitive and DIDS insensitive pathways for Cl- at the apical membrane of human intestinal T84 epithelial cells.
|Number of pages||7|
|Journal||Biochimica et Biophysica Acta|
|Publication status||Published - 17 Feb 1994|
- 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
- Cell Line
- Hypotonic Solutions
- Membrane Potentials
- Time Factors
- Vasoactive Intestinal Peptide
McEwan, G. T., Hirst, B. H., & Simmons, N. L. (1994). Carbachol stimulates Cl- secretion via activation of two distinct apical Cl- pathways in cultured human T84 intestinal epithelial monolayers. Biochimica et Biophysica Acta, 1220(3), 241-7.