Carbachol stimulates Cl- secretion via activation of two distinct apical Cl- pathways in cultured human T84 intestinal epithelial monolayers

G T McEwan, B H Hirst, N L Simmons

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The mode of action of carbachol in stimulation of transepithelial Cl- secretion in intact human intestinal T84 epithelial monolayers has been investigated in order to determine whether a DIDS-insensitive exit pathway (via CFTR) coexists with a DIDS-sensitive exit pathway at the apical membrane. Carbachol stimulates a transient inward Isc due to Cl- secretion whose magnitude is related to the basal level of inward Isc. The inward current responses to both carbachol and hypo-osmotic media are abolished in nominally Ca(2+)-free media. The action of apical DIDS (100 microM) upon carbachol-stimulated Isc depends on the initial value of the basal Isc. At basal Isc levels 10 microA cm-2, the carbachol stimulation of inward Isc was largely insensitive to 100 microM DIDS. The data are consistent with the participation of both DIDS-sensitive and DIDS insensitive pathways for Cl- at the apical membrane of human intestinal T84 epithelial cells.
Original languageEnglish
Pages (from-to)241-7
Number of pages7
JournalBiochimica et Biophysica Acta
Issue number3
Publication statusPublished - 17 Feb 1994



  • 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
  • Calcium
  • Carbachol
  • Cell Line
  • Chlorides
  • Epithelium
  • Humans
  • Hypotonic Solutions
  • Intestines
  • Kinetics
  • Membrane Potentials
  • Time Factors
  • Vasoactive Intestinal Peptide

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