Abstract
The mode of action of carbachol in stimulation of transepithelial Cl- secretion in intact human intestinal T84 epithelial monolayers has been investigated in order to determine whether a DIDS-insensitive exit pathway (via CFTR) coexists with a DIDS-sensitive exit pathway at the apical membrane. Carbachol stimulates a transient inward Isc due to Cl- secretion whose magnitude is related to the basal level of inward Isc. The inward current responses to both carbachol and hypo-osmotic media are abolished in nominally Ca(2+)-free media. The action of apical DIDS (100 microM) upon carbachol-stimulated Isc depends on the initial value of the basal Isc. At basal Isc levels 10 microA cm-2, the carbachol stimulation of inward Isc was largely insensitive to 100 microM DIDS. The data are consistent with the participation of both DIDS-sensitive and DIDS insensitive pathways for Cl- at the apical membrane of human intestinal T84 epithelial cells.
Original language | English |
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Pages (from-to) | 241-7 |
Number of pages | 7 |
Journal | Biochimica et Biophysica Acta |
Volume | 1220 |
Issue number | 3 |
Publication status | Published - 17 Feb 1994 |
Keywords
- 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
- Calcium
- Carbachol
- Cell Line
- Chlorides
- Epithelium
- Humans
- Hypotonic Solutions
- Intestines
- Kinetics
- Membrane Potentials
- Time Factors
- Vasoactive Intestinal Peptide