Carbon and oxygen controls on N20 and N2 production during nitrate reduction

N. Morley, E. M. Baggs

Research output: Contribution to journalArticle

109 Citations (Scopus)

Abstract

Here we provide evidence that the form of carbon compound and O-2 concentration exert an inter-related regulation on the production and reduction of N2O in soil. 6.7 mM D-glucose, 6.7 mM D-mannitol, 8 mM L-glutamic acid or 10 mM butyrate (all equivalent to 0.48 g Cl-1) were applied to slurries of a sandy loam soil. At the start of the experiment headspace O-2 concentrations were established at similar to 2%, 10% and 21% O-2 v/v for each C treatment, and 2 mM (KNO3)-N-15 (25 atom % excess N-15) was applied, enabling quantification of N-15-N-2 production, N-15-(N2O-to-N-2) ratios and DNRA. The form of C compound was most important in the initially oxic (21% O-2 v/v) soils, where addition of butyrate and glutamic acid resulted in greater N2O production (0.61 and 0.3 mu g N2O-N g(-1) soil for butyrate and glutamic acid, respectively) than the addition of carbohydrates (glucose and mannitol). Although, there was no significant effect of C compound at low initial O-2 concentrations (similar to 2% O-2 v/v), production of N-15-N-2 was greatest where headspace O-2 concentrations were initially, or fallen to, similar to 2% 02 v/v, with greatest reduction of N2O and lowering N-15-(N2O-to-N-2) ratios (similar to 0-0.27). This may reflect that the effect of C is indirect through stimulation of heterotrophic respiration, lowering O-2 concentrations, providing sub-oxic conditions for dissimilatory nitrate reduction pathways. Addition of carbohydrates (glucose and mannitol) also resulted in greatest recovery of N-15 in NH4+ from applied N-15-NO3-, indicative of the occurrence of DNRA, even in the slurries with initial 10% and 21% O-2 v/v concentrations. Our N-15 approach has provided the first direct evidence for enhancement of N2O reduction in the presence of carbohydrates and the dual regulation of C compound and O-2 concentration on N2O production and reduction, which has implications for management of N2O emissions through changing C inputs (exudates, rhizodeposition, residues) with plant species of differing C traits, or through plant breeding. (C) 2010 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)1864-1871
Number of pages8
JournalSoil Biology and Biochemistry
Volume42
Issue number10
Early online date23 Jul 2010
DOIs
Publication statusPublished - Oct 2010

Keywords

  • Carbon
  • Denitrification
  • Dinitrogen
  • DNRA
  • Nitrous oxide
  • Oxygen
  • atmospheric nitrous-oxide
  • dissimilatory reduction
  • paracoccus-pantotrophus
  • bacterial communities
  • soil denitrification
  • barley rhizosphere
  • ammonium
  • glucose
  • rates
  • expression

Cite this

Carbon and oxygen controls on N20 and N2 production during nitrate reduction. / Morley, N.; Baggs, E. M.

In: Soil Biology and Biochemistry, Vol. 42, No. 10, 10.2010, p. 1864-1871.

Research output: Contribution to journalArticle

Morley, N. ; Baggs, E. M. / Carbon and oxygen controls on N20 and N2 production during nitrate reduction. In: Soil Biology and Biochemistry. 2010 ; Vol. 42, No. 10. pp. 1864-1871.
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T1 - Carbon and oxygen controls on N20 and N2 production during nitrate reduction

AU - Morley, N.

AU - Baggs, E. M.

N1 - A paid open access option is available for this journal. Voluntary deposit by author of pre-print allowed on Institutions open scholarly website and pre-print servers Voluntary deposit by author of authors post-print allowed on institutions open scholarly website including Institutional Repository Deposit due to Funding Body, Institutional and Governmental mandate only allowed where separate agreement between repository and publisher exists Set statement to accompany deposit Published source must be acknowledged Must link to journal home page or articles' DOI Publisher's version/PDF cannot be used Articles in some journals can be made Open Access on payment of additional charge NIH Authors articles will be submitted to PubMed Central after <num>12</num> <period units="month">months</period> Authors who are required to deposit in subject-based repositories may also use Sponsorship Option

PY - 2010/10

Y1 - 2010/10

N2 - Here we provide evidence that the form of carbon compound and O-2 concentration exert an inter-related regulation on the production and reduction of N2O in soil. 6.7 mM D-glucose, 6.7 mM D-mannitol, 8 mM L-glutamic acid or 10 mM butyrate (all equivalent to 0.48 g Cl-1) were applied to slurries of a sandy loam soil. At the start of the experiment headspace O-2 concentrations were established at similar to 2%, 10% and 21% O-2 v/v for each C treatment, and 2 mM (KNO3)-N-15 (25 atom % excess N-15) was applied, enabling quantification of N-15-N-2 production, N-15-(N2O-to-N-2) ratios and DNRA. The form of C compound was most important in the initially oxic (21% O-2 v/v) soils, where addition of butyrate and glutamic acid resulted in greater N2O production (0.61 and 0.3 mu g N2O-N g(-1) soil for butyrate and glutamic acid, respectively) than the addition of carbohydrates (glucose and mannitol). Although, there was no significant effect of C compound at low initial O-2 concentrations (similar to 2% O-2 v/v), production of N-15-N-2 was greatest where headspace O-2 concentrations were initially, or fallen to, similar to 2% 02 v/v, with greatest reduction of N2O and lowering N-15-(N2O-to-N-2) ratios (similar to 0-0.27). This may reflect that the effect of C is indirect through stimulation of heterotrophic respiration, lowering O-2 concentrations, providing sub-oxic conditions for dissimilatory nitrate reduction pathways. Addition of carbohydrates (glucose and mannitol) also resulted in greatest recovery of N-15 in NH4+ from applied N-15-NO3-, indicative of the occurrence of DNRA, even in the slurries with initial 10% and 21% O-2 v/v concentrations. Our N-15 approach has provided the first direct evidence for enhancement of N2O reduction in the presence of carbohydrates and the dual regulation of C compound and O-2 concentration on N2O production and reduction, which has implications for management of N2O emissions through changing C inputs (exudates, rhizodeposition, residues) with plant species of differing C traits, or through plant breeding. (C) 2010 Elsevier Ltd. All rights reserved.

AB - Here we provide evidence that the form of carbon compound and O-2 concentration exert an inter-related regulation on the production and reduction of N2O in soil. 6.7 mM D-glucose, 6.7 mM D-mannitol, 8 mM L-glutamic acid or 10 mM butyrate (all equivalent to 0.48 g Cl-1) were applied to slurries of a sandy loam soil. At the start of the experiment headspace O-2 concentrations were established at similar to 2%, 10% and 21% O-2 v/v for each C treatment, and 2 mM (KNO3)-N-15 (25 atom % excess N-15) was applied, enabling quantification of N-15-N-2 production, N-15-(N2O-to-N-2) ratios and DNRA. The form of C compound was most important in the initially oxic (21% O-2 v/v) soils, where addition of butyrate and glutamic acid resulted in greater N2O production (0.61 and 0.3 mu g N2O-N g(-1) soil for butyrate and glutamic acid, respectively) than the addition of carbohydrates (glucose and mannitol). Although, there was no significant effect of C compound at low initial O-2 concentrations (similar to 2% O-2 v/v), production of N-15-N-2 was greatest where headspace O-2 concentrations were initially, or fallen to, similar to 2% 02 v/v, with greatest reduction of N2O and lowering N-15-(N2O-to-N-2) ratios (similar to 0-0.27). This may reflect that the effect of C is indirect through stimulation of heterotrophic respiration, lowering O-2 concentrations, providing sub-oxic conditions for dissimilatory nitrate reduction pathways. Addition of carbohydrates (glucose and mannitol) also resulted in greatest recovery of N-15 in NH4+ from applied N-15-NO3-, indicative of the occurrence of DNRA, even in the slurries with initial 10% and 21% O-2 v/v concentrations. Our N-15 approach has provided the first direct evidence for enhancement of N2O reduction in the presence of carbohydrates and the dual regulation of C compound and O-2 concentration on N2O production and reduction, which has implications for management of N2O emissions through changing C inputs (exudates, rhizodeposition, residues) with plant species of differing C traits, or through plant breeding. (C) 2010 Elsevier Ltd. All rights reserved.

KW - Carbon

KW - Denitrification

KW - Dinitrogen

KW - DNRA

KW - Nitrous oxide

KW - Oxygen

KW - atmospheric nitrous-oxide

KW - dissimilatory reduction

KW - paracoccus-pantotrophus

KW - bacterial communities

KW - soil denitrification

KW - barley rhizosphere

KW - ammonium

KW - glucose

KW - rates

KW - expression

U2 - 10.1016/j.soilbio.2010.07.008

DO - 10.1016/j.soilbio.2010.07.008

M3 - Article

VL - 42

SP - 1864

EP - 1871

JO - Soil Biology and Biochemistry

JF - Soil Biology and Biochemistry

SN - 0038-0717

IS - 10

ER -