Cardiomyocyte Differentiation from Human Embryonic Stem Cells

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4 Citations (Scopus)


In vitro generated human cardiomyocytes hold the ultimate promise for heart patients for repair of injured or diseased myocardium, but they also provide experimental models for studying normal cardiomyocyte development, for disease modelling and for drug development. Here we provide reliable protocols for differentiation of human embryonic stem cells into functional cardiomyocytes, together with Notes about trouble shooting and optimising such protocols for specific cell lines. This chapter also briefly discusses other published protocols and those further adapted for differentiation of induced Pluripotent Stem Cells into cardiomyocytes.
Original languageEnglish
Title of host publicationExperimental Models of Cardiovascular Diseases
EditorsK Ishikawa
Number of pages12
ISBN (Electronic)978-1-4939-8597-5
ISBN (Print)978-1-4939-8596-8
Publication statusPublished - 2018

Publication series

NameMethods in Molecular Biology
ISSN (Electronic)1064-3745


  • human embryonic stem cells
  • human pluripotent stem cells
  • human induced pluripotent stem cells
  • human cardiomyocytes
  • human heart development
  • in vitro differentiation
  • Wnt signalling


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