Cardiomyocyte Differentiation from Human Embryonic Stem Cells

Silvia Mazzotta, Adam T Lynch, Stefan Hoppler

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

In vitro generated human cardiomyocytes hold the ultimate promise for heart patients for repair of injured or diseased myocardium, but they also provide experimental models for studying normal cardiomyocyte development, for disease modelling and for drug development. Here we provide reliable protocols for differentiation of human embryonic stem cells into functional cardiomyocytes, together with Notes about trouble shooting and optimising such protocols for specific cell lines. This chapter also briefly discusses other published protocols and those further adapted for differentiation of induced Pluripotent Stem Cells into cardiomyocytes.
Original languageEnglish
Title of host publicationExperimental Models of Cardiovascular Diseases
EditorsK Ishikawa
PublisherSpringer
Pages67-78
Number of pages12
ISBN (Electronic)978-1-4939-8597-5
ISBN (Print)978-1-4939-8596-8
DOIs
Publication statusPublished - 2018

Publication series

NameMethods in Molecular Biology
PublisherSpringer
ISSN (Electronic)1064-3745

Fingerprint

Cardiac Myocytes
Induced Pluripotent Stem Cells
Drug Design
Myocardium
Theoretical Models
Cell Line
Human Embryonic Stem Cells

Keywords

  • human embryonic stem cells
  • human pluripotent stem cells
  • human induced pluripotent stem cells
  • human cardiomyocytes
  • human heart development
  • in vitro differentiation
  • Wnt signalling

Cite this

Mazzotta, S., Lynch, A. T., & Hoppler, S. (2018). Cardiomyocyte Differentiation from Human Embryonic Stem Cells. In K. Ishikawa (Ed.), Experimental Models of Cardiovascular Diseases (pp. 67-78). (Methods in Molecular Biology). Springer . https://doi.org/10.1007/978-1-4939-8597-5_5

Cardiomyocyte Differentiation from Human Embryonic Stem Cells. / Mazzotta, Silvia; Lynch, Adam T; Hoppler, Stefan.

Experimental Models of Cardiovascular Diseases. ed. / K Ishikawa . Springer , 2018. p. 67-78 (Methods in Molecular Biology).

Research output: Chapter in Book/Report/Conference proceedingChapter

Mazzotta, S, Lynch, AT & Hoppler, S 2018, Cardiomyocyte Differentiation from Human Embryonic Stem Cells. in K Ishikawa (ed.), Experimental Models of Cardiovascular Diseases. Methods in Molecular Biology, Springer , pp. 67-78. https://doi.org/10.1007/978-1-4939-8597-5_5
Mazzotta S, Lynch AT, Hoppler S. Cardiomyocyte Differentiation from Human Embryonic Stem Cells. In Ishikawa K, editor, Experimental Models of Cardiovascular Diseases. Springer . 2018. p. 67-78. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-8597-5_5
Mazzotta, Silvia ; Lynch, Adam T ; Hoppler, Stefan. / Cardiomyocyte Differentiation from Human Embryonic Stem Cells. Experimental Models of Cardiovascular Diseases. editor / K Ishikawa . Springer , 2018. pp. 67-78 (Methods in Molecular Biology).
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