Cardiomyocyte Differentiation from Mouse Embryonic Stem Cells

Adam T Lynch, Silvia Mazzotta, Stefan Hoppler

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

In vitro generated mammalian cardiomyocytes provide experimental models for studying normal mammalian cardiomyocyte development, for disease modelling and for drug development. They also promise to inform future therapeutic strategies for repair of injured or diseased myocardium. Here we provide reliable protocols for differentiation of mouse embryonic stem cells into functional cardiomyocytes, together with Notes about trouble shooting and optimising such protocols for specific cell lines.
Original languageEnglish
Title of host publicationExperimental Models of Cardiovascular Disease
Subtitle of host publicationMethods and Protocols
EditorsK Ishikawa
PublisherSpringer
Pages55-66
Number of pages12
ISBN (Print)978-1-4939-8596-8
DOIs
Publication statusE-pub ahead of print - 10 Jul 2018

Publication series

NameMethods in Molecular Biology Series
PublisherSpringer
Volume1816
ISSN (Electronic)1064-3745

Fingerprint

Cardiac Myocytes
Drug Design
Myocardium
Theoretical Models
Cell Line
Mouse Embryonic Stem Cells
Therapeutics

Keywords

  • mouse embryonic stem cell
  • cardiomyocytes
  • heart
  • in vitro differentiation

Cite this

Lynch, A. T., Mazzotta, S., & Hoppler, S. (2018). Cardiomyocyte Differentiation from Mouse Embryonic Stem Cells. In K. Ishikawa (Ed.), Experimental Models of Cardiovascular Disease: Methods and Protocols (pp. 55-66). (Methods in Molecular Biology Series; Vol. 1816). Springer . https://doi.org/10.1007/978-1-4939-8597-5_4

Cardiomyocyte Differentiation from Mouse Embryonic Stem Cells. / Lynch, Adam T; Mazzotta, Silvia; Hoppler, Stefan.

Experimental Models of Cardiovascular Disease: Methods and Protocols. ed. / K Ishikawa. Springer , 2018. p. 55-66 (Methods in Molecular Biology Series; Vol. 1816).

Research output: Chapter in Book/Report/Conference proceedingChapter

Lynch, AT, Mazzotta, S & Hoppler, S 2018, Cardiomyocyte Differentiation from Mouse Embryonic Stem Cells. in K Ishikawa (ed.), Experimental Models of Cardiovascular Disease: Methods and Protocols. Methods in Molecular Biology Series, vol. 1816, Springer , pp. 55-66. https://doi.org/10.1007/978-1-4939-8597-5_4
Lynch AT, Mazzotta S, Hoppler S. Cardiomyocyte Differentiation from Mouse Embryonic Stem Cells. In Ishikawa K, editor, Experimental Models of Cardiovascular Disease: Methods and Protocols. Springer . 2018. p. 55-66. (Methods in Molecular Biology Series). https://doi.org/10.1007/978-1-4939-8597-5_4
Lynch, Adam T ; Mazzotta, Silvia ; Hoppler, Stefan. / Cardiomyocyte Differentiation from Mouse Embryonic Stem Cells. Experimental Models of Cardiovascular Disease: Methods and Protocols. editor / K Ishikawa. Springer , 2018. pp. 55-66 (Methods in Molecular Biology Series).
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