Catalase T-deficient fission yeast meiocytes show resistance to ionizing radiation

Razan Muhtadi, Alexander Lorenz, Samantha Mpaulo, Christian Siebenwirth, Harry Scherthan* (Corresponding Author)

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Environmental stress, reactive oxygen species (ROS), or ionizing radiation (IR) can induce adverse effects in organisms and their cells, including mutations and premature aging. DNA damage and its faulty repair can lead to cell death or promote cancer through the accumulation of mutations. Misrepair in germ cells is particularly dangerous as it may lead to alterations in developmental programs and genetic disease in the offspring. DNA damage pathways and radical defense mechanisms mediate resistance to genotoxic stresses. Here, we investigated, in the fission yeast Schizosaccharomyces pombe, the role of the H2O2-detoxifying enzyme cytosolic catalase T (Ctt1) and the Fe2+/Mn2+ symporter Pcl1 in protecting meiotic chromosome dynamics and gamete formation from radicals generated by ROS and IR. We found that wild-type and pcl1-deficient cells respond similarly to X ray doses of up to 300 Gy, while ctt1∆ meiocytes showed a moderate sensitivity to IR but a hypersensitivity to hydrogen peroxide with cells dying at >0.4 mM H2O2. Meiocytes deficient for pcl1, on the other hand, showed a resistance to hydrogen peroxide similar to that of the wild type, surviving doses >40 mM. In all, it appears that in the absence of the main H2O2-detoxifying pathway S. pombe meiocytes are able to survive significant doses of IR-induced radicals.
Original languageEnglish
Article number881
Pages (from-to)1-17
Number of pages17
JournalAntioxidants
Volume9
Issue number9
Early online date17 Sep 2020
DOIs
Publication statusPublished - Sep 2020

Keywords

  • chromosome mobility
  • Ctt1
  • horsetail movemen
  • ionizing radiation
  • meiosis
  • radical stress
  • live‐cell microscopy
  • Pcl1
  • ROS
  • Schizosaccharomyces pombe
  • sporulation
  • Radical stress
  • Meiosis
  • Live‐cell microscopy
  • Horsetail movement
  • Ionizing radiation
  • Sporulation
  • Chromosome mobility

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