CD13/aminopeptidase N is a negative regulator of mast cell activation

Julia S. Zotz, Florian Wölbing, Caroline Lassnig, Marlies Kauffmann, Uwe Schulte, Andreas Friedrich Kolb, Bruce Whitelaw, Mathias Müller, Tilo Biedermann, Michael Huber

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Antigen-induced mast cell (MC) activation via cross-linking of IgE-bound high-affinity receptors for IgE (FcεRI) underlies type I allergy and anaphylactic shock. Comprehensive knowledge of FcεRI regulation is thus required. We have identified a functional interaction between FcεRI and CD13 in murine MCs. Antigen-triggered activation of IgE-loaded FcεRI results in cocapping and cointernalization of CD13 and equivalent internalization rates of up to 40%. Cointernalization is not unspecific, because ligand-driven KIT internalization is not accompanied by CD13 internalization. Moreover, antibody-mediated cross-linking of CD13 causes IL-6 production in an FcεRI-dependent manner. These data are indicative of a functional interaction between FcεRI and CD13 on MCs. To determine the role of this interaction, CD13-deficient bone marrow-derived MCs (BMMCs) were analyzed. Intriguingly, antigen stimulation of CD13-deficient BMMCs results in significantly increased degranulation and proinflammatory cytokine production compared to wild-type cells. Furthermore, in a low-dose model of passive systemic anaphylaxis, antigen-dependent decrease in body temperature, reflecting the anaphylactic reaction, is substantially enhanced by the CD13 inhibitor bestatin (-5.9 ± 0.6°C) and by CD13 deficiency (-8.8 ± 0.6°C) in contrast to controls (-1.2 ± 1.97°C). Importantly, bestatin does not aggravate anaphylaxis in CD13-deficient mice. Thus, we have identified CD13 as a novel negative regulator of MC activation in vitro and in vivo
Original languageEnglish
Pages (from-to)2225-2235
Number of pages11
JournalThe FASEB Journal
Volume30
Issue number6
Early online date2 Mar 2016
DOIs
Publication statusPublished - Jun 2016

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CD13 Antigens
Anaphylaxis
Mast Cells
Chemical activation
Antigens
Immunoglobulin E
Bone
Bone Marrow
Allergies
IgE Receptors
Body Temperature
Interleukin-6
Hypersensitivity
Cytokines
Ligands
Antibodies
Temperature
ubenimex

Keywords

  • IgE receptor
  • proinflammatory cytokines
  • degranulation
  • passive systemic anaphylaxis
  • receptor internalization

Cite this

Zotz, J. S., Wölbing, F., Lassnig, C., Kauffmann, M., Schulte, U., Kolb, A. F., ... Huber, M. (2016). CD13/aminopeptidase N is a negative regulator of mast cell activation. The FASEB Journal, 30(6), 2225-2235. https://doi.org/10.1096/fj.201600278

CD13/aminopeptidase N is a negative regulator of mast cell activation. / Zotz, Julia S. ; Wölbing, Florian ; Lassnig, Caroline; Kauffmann, Marlies ; Schulte, Uwe; Kolb, Andreas Friedrich; Whitelaw, Bruce; Müller, Mathias; Biedermann, Tilo; Huber, Michael.

In: The FASEB Journal, Vol. 30, No. 6, 06.2016, p. 2225-2235.

Research output: Contribution to journalArticle

Zotz, JS, Wölbing, F, Lassnig, C, Kauffmann, M, Schulte, U, Kolb, AF, Whitelaw, B, Müller, M, Biedermann, T & Huber, M 2016, 'CD13/aminopeptidase N is a negative regulator of mast cell activation', The FASEB Journal, vol. 30, no. 6, pp. 2225-2235. https://doi.org/10.1096/fj.201600278
Zotz JS, Wölbing F, Lassnig C, Kauffmann M, Schulte U, Kolb AF et al. CD13/aminopeptidase N is a negative regulator of mast cell activation. The FASEB Journal. 2016 Jun;30(6):2225-2235. https://doi.org/10.1096/fj.201600278
Zotz, Julia S. ; Wölbing, Florian ; Lassnig, Caroline ; Kauffmann, Marlies ; Schulte, Uwe ; Kolb, Andreas Friedrich ; Whitelaw, Bruce ; Müller, Mathias ; Biedermann, Tilo ; Huber, Michael. / CD13/aminopeptidase N is a negative regulator of mast cell activation. In: The FASEB Journal. 2016 ; Vol. 30, No. 6. pp. 2225-2235.
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abstract = "Antigen-induced mast cell (MC) activation via cross-linking of IgE-bound high-affinity receptors for IgE (FcεRI) underlies type I allergy and anaphylactic shock. Comprehensive knowledge of FcεRI regulation is thus required. We have identified a functional interaction between FcεRI and CD13 in murine MCs. Antigen-triggered activation of IgE-loaded FcεRI results in cocapping and cointernalization of CD13 and equivalent internalization rates of up to 40{\%}. Cointernalization is not unspecific, because ligand-driven KIT internalization is not accompanied by CD13 internalization. Moreover, antibody-mediated cross-linking of CD13 causes IL-6 production in an FcεRI-dependent manner. These data are indicative of a functional interaction between FcεRI and CD13 on MCs. To determine the role of this interaction, CD13-deficient bone marrow-derived MCs (BMMCs) were analyzed. Intriguingly, antigen stimulation of CD13-deficient BMMCs results in significantly increased degranulation and proinflammatory cytokine production compared to wild-type cells. Furthermore, in a low-dose model of passive systemic anaphylaxis, antigen-dependent decrease in body temperature, reflecting the anaphylactic reaction, is substantially enhanced by the CD13 inhibitor bestatin (-5.9 ± 0.6°C) and by CD13 deficiency (-8.8 ± 0.6°C) in contrast to controls (-1.2 ± 1.97°C). Importantly, bestatin does not aggravate anaphylaxis in CD13-deficient mice. Thus, we have identified CD13 as a novel negative regulator of MC activation in vitro and in vivo",
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AU - Wölbing, Florian

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AU - Kolb, Andreas Friedrich

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AU - Müller, Mathias

AU - Biedermann, Tilo

AU - Huber, Michael

N1 - The authors acknowledge the excellent technical assistance of Kerstin Fehrenbach (Max Planck Institute for Immunobiology, Freiburg, Germany), Sabrina Schmohl (Department of Dermatology, Eberhard Karls University, Tübingen, Germany), and Yvonne Postma (Department of Dermatology, Eberhard Karls University). The authors are grateful to Dr. Gessner (Hannover Medical School, Hannover, Germany) for providing bone marrow from FcRγ+/+ and FcRγ−/− littermates. This work was supported by the Grants HU794/5-1 (to M.H.), BI696/3-3 (to T.B.), SPP1394 (to M.H. and T.B.), and SFB685 (A06; to T.B.) from the Deutsche Forschungsgemeinschaft, as well as BM-WFaGEN-AU project Austromouse, Austrian Federal Ministry Research and Science, and Austrian Science Fund Grant SFB-F28 (to M.M.).

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N2 - Antigen-induced mast cell (MC) activation via cross-linking of IgE-bound high-affinity receptors for IgE (FcεRI) underlies type I allergy and anaphylactic shock. Comprehensive knowledge of FcεRI regulation is thus required. We have identified a functional interaction between FcεRI and CD13 in murine MCs. Antigen-triggered activation of IgE-loaded FcεRI results in cocapping and cointernalization of CD13 and equivalent internalization rates of up to 40%. Cointernalization is not unspecific, because ligand-driven KIT internalization is not accompanied by CD13 internalization. Moreover, antibody-mediated cross-linking of CD13 causes IL-6 production in an FcεRI-dependent manner. These data are indicative of a functional interaction between FcεRI and CD13 on MCs. To determine the role of this interaction, CD13-deficient bone marrow-derived MCs (BMMCs) were analyzed. Intriguingly, antigen stimulation of CD13-deficient BMMCs results in significantly increased degranulation and proinflammatory cytokine production compared to wild-type cells. Furthermore, in a low-dose model of passive systemic anaphylaxis, antigen-dependent decrease in body temperature, reflecting the anaphylactic reaction, is substantially enhanced by the CD13 inhibitor bestatin (-5.9 ± 0.6°C) and by CD13 deficiency (-8.8 ± 0.6°C) in contrast to controls (-1.2 ± 1.97°C). Importantly, bestatin does not aggravate anaphylaxis in CD13-deficient mice. Thus, we have identified CD13 as a novel negative regulator of MC activation in vitro and in vivo

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KW - proinflammatory cytokines

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