CD31 delays phagocyte membrane repolarization to promote efficient binding of apoptotic cells

E.F. Vernon-Wilson, F. Auradé, L. Tian, I.C.M. Rowe, M.J. Shipston, J. Savill, S.B. Brown

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)


Homophilic ligation of CD31, a member of the Ig superfamily of adhesion receptors, promotes macrophage clearance of apoptotic leukocytes by a mechanism hitherto not described. In studying CD31-dependent regulation of β1-integrin binding of fibronectin-coated Latex™ beads, we discovered a role for the voltage-gated potassium channel ether-à-go-go-related gene (ERG) as a downstream effector of CD31 signaling. ERG was identified by tandem mass spectrometry as a 140-kDa protein, which was selectively modified with biotin following the targeted delivery of a biotin-transfer reagent to CD31 using Fab fragments of an anti-CD31 mAb. Similar results were obtained with macrophages but not K562 cells, expressing a truncated cytoplasmic tail of CD31, which failed to regulate bead binding. Colocalization of CD31 with ERG was confirmed by immunofluorescence for K562 cells and macrophages. We now demonstrate that the resting membrane potential of macrophages is depolarized on contact with apoptotic cells and that CD31 inhibits the ERG current, which would otherwise function to repolarize. Sustained depolarization favored the firm binding of phagocytic targets, a prerequisite for efficient engulfment. Our results identify ERG as a downstream effector of CD31 in the regulation of integrin-dependent binding of apoptotic cells by macrophages.
Original languageEnglish
Pages (from-to)1278-1288
JournalJournal of Leukocyte Biology
Issue number5
Publication statusPublished - 2007


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