CD4(+)CD25(+) T regulatory cells induced by LPS-activated bone marrow dendritic cells suppress experimental autoimmune uveoretinitis in vivo

Kirsten Siepmann, Sabine Biester, Jarmila Plskova, Elizabeth Muckersie, Linda Duncan, John V. Forrester

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Tolerance-inducing DC are considered to be less mature than immunogenic DC, but the conditions promoting a less mature DC phenotype are not clear. We have previously shown that lipopolysaccharide (LPS) can have differential effects on DC function depending on the timing of DC exposure to LPS. Here, we show that early LPS-activated bone marrow derived DC (early DC, eDC), when administered subcutaneously to mice in vivo, promote tolerance to EAU induced via immunisation with interphotoreceptor retinol binding protein (IRBP) peptide 161-180. The effect correlates with the failure of eDC to secrete IL-12, and appears to be mediated in part via expansion of naturally occurring CD4(+) CD25(+) T regulatory cells (Tregs), which also mediate suppression of EAU on adoptive transfer to naive mice followed by immunization with autoantigen.

Immature DC were prepared from BMDC cultures. Early DC (eDC) and late DC (lDC) for tolerance experiments were obtained by differential timing of LPS addition and their cytokine secretion profile was analyzed. eDC and lDC were subcutaneously injected into mice. From the dLN CD4(+) CD25(+) GITR(+) T regulatory cells found to express FoxP3 were isolated and transferred into mice prior to immunisation with IRBP. The immune response was scored by histopathology. Tregs were characterized in vitro by intracellular staining, cytokine secretion assay and transwell experiments.

eDC secrete IL-10 but no IL-12 or IFN gamma. When injected subcutaneously into naive mice, they expand the population of CD4(+) CD25(+high) GITR(+) T cells expressing FoxP3 in the dLN, thus increasing the total number of IL-10 producing cells. eDC induced Tregs inhibit CD4(+) CD25(-) T effector cell proliferation by a contact dependent process, and both eDC and Tregs suppress retinal damage when adoptively transferred.

We suggest that DC maturation may be necessary for both tolerance and immunity, but differential levels of activation and/or cytokine production direct the outcome of DC-T cell interaction and this is determined by IL-12 production. T regulatory cells induced in vivo by contact with eDC are able to suppress disease in the EAU model by adoptive transfer.

Original languageEnglish
Pages (from-to)221-229
Number of pages9
JournalGraefe's Archive for Clinical and Experimental Ophthalmology
Volume245
Issue number2
Early online date2 Jun 2006
DOIs
Publication statusPublished - Feb 2007

Keywords

  • dendritic cells
  • experimental autoimmune uveoretinitis
  • lipopolysaccharide
  • T regulatory cells
  • toll-like receptors
  • signaling pathway
  • immune-responses
  • IL-12 production
  • GM-CSF
  • tolerance
  • induction
  • mice
  • inhibition
  • molecule

Cite this

CD4(+)CD25(+) T regulatory cells induced by LPS-activated bone marrow dendritic cells suppress experimental autoimmune uveoretinitis in vivo. / Siepmann, Kirsten; Biester, Sabine; Plskova, Jarmila; Muckersie, Elizabeth; Duncan, Linda; Forrester, John V.

In: Graefe's Archive for Clinical and Experimental Ophthalmology, Vol. 245, No. 2, 02.2007, p. 221-229.

Research output: Contribution to journalArticle

Siepmann, Kirsten ; Biester, Sabine ; Plskova, Jarmila ; Muckersie, Elizabeth ; Duncan, Linda ; Forrester, John V. / CD4(+)CD25(+) T regulatory cells induced by LPS-activated bone marrow dendritic cells suppress experimental autoimmune uveoretinitis in vivo. In: Graefe's Archive for Clinical and Experimental Ophthalmology. 2007 ; Vol. 245, No. 2. pp. 221-229.
@article{f3ec3d7c121c4837bd32097328ad7b9c,
title = "CD4(+)CD25(+) T regulatory cells induced by LPS-activated bone marrow dendritic cells suppress experimental autoimmune uveoretinitis in vivo",
abstract = "Tolerance-inducing DC are considered to be less mature than immunogenic DC, but the conditions promoting a less mature DC phenotype are not clear. We have previously shown that lipopolysaccharide (LPS) can have differential effects on DC function depending on the timing of DC exposure to LPS. Here, we show that early LPS-activated bone marrow derived DC (early DC, eDC), when administered subcutaneously to mice in vivo, promote tolerance to EAU induced via immunisation with interphotoreceptor retinol binding protein (IRBP) peptide 161-180. The effect correlates with the failure of eDC to secrete IL-12, and appears to be mediated in part via expansion of naturally occurring CD4(+) CD25(+) T regulatory cells (Tregs), which also mediate suppression of EAU on adoptive transfer to naive mice followed by immunization with autoantigen.Immature DC were prepared from BMDC cultures. Early DC (eDC) and late DC (lDC) for tolerance experiments were obtained by differential timing of LPS addition and their cytokine secretion profile was analyzed. eDC and lDC were subcutaneously injected into mice. From the dLN CD4(+) CD25(+) GITR(+) T regulatory cells found to express FoxP3 were isolated and transferred into mice prior to immunisation with IRBP. The immune response was scored by histopathology. Tregs were characterized in vitro by intracellular staining, cytokine secretion assay and transwell experiments.eDC secrete IL-10 but no IL-12 or IFN gamma. When injected subcutaneously into naive mice, they expand the population of CD4(+) CD25(+high) GITR(+) T cells expressing FoxP3 in the dLN, thus increasing the total number of IL-10 producing cells. eDC induced Tregs inhibit CD4(+) CD25(-) T effector cell proliferation by a contact dependent process, and both eDC and Tregs suppress retinal damage when adoptively transferred.We suggest that DC maturation may be necessary for both tolerance and immunity, but differential levels of activation and/or cytokine production direct the outcome of DC-T cell interaction and this is determined by IL-12 production. T regulatory cells induced in vivo by contact with eDC are able to suppress disease in the EAU model by adoptive transfer.",
keywords = "dendritic cells, experimental autoimmune uveoretinitis, lipopolysaccharide, T regulatory cells, toll-like receptors, signaling pathway, immune-responses, IL-12 production, GM-CSF, tolerance, induction, mice , inhibition, molecule",
author = "Kirsten Siepmann and Sabine Biester and Jarmila Plskova and Elizabeth Muckersie and Linda Duncan and Forrester, {John V.}",
year = "2007",
month = "2",
doi = "10.1007/s00417-006-0356-9",
language = "English",
volume = "245",
pages = "221--229",
journal = "Graefe's Archive for Clinical and Experimental Ophthalmology",
issn = "0721-832X",
publisher = "Springer Verlag",
number = "2",

}

TY - JOUR

T1 - CD4(+)CD25(+) T regulatory cells induced by LPS-activated bone marrow dendritic cells suppress experimental autoimmune uveoretinitis in vivo

AU - Siepmann, Kirsten

AU - Biester, Sabine

AU - Plskova, Jarmila

AU - Muckersie, Elizabeth

AU - Duncan, Linda

AU - Forrester, John V.

PY - 2007/2

Y1 - 2007/2

N2 - Tolerance-inducing DC are considered to be less mature than immunogenic DC, but the conditions promoting a less mature DC phenotype are not clear. We have previously shown that lipopolysaccharide (LPS) can have differential effects on DC function depending on the timing of DC exposure to LPS. Here, we show that early LPS-activated bone marrow derived DC (early DC, eDC), when administered subcutaneously to mice in vivo, promote tolerance to EAU induced via immunisation with interphotoreceptor retinol binding protein (IRBP) peptide 161-180. The effect correlates with the failure of eDC to secrete IL-12, and appears to be mediated in part via expansion of naturally occurring CD4(+) CD25(+) T regulatory cells (Tregs), which also mediate suppression of EAU on adoptive transfer to naive mice followed by immunization with autoantigen.Immature DC were prepared from BMDC cultures. Early DC (eDC) and late DC (lDC) for tolerance experiments were obtained by differential timing of LPS addition and their cytokine secretion profile was analyzed. eDC and lDC were subcutaneously injected into mice. From the dLN CD4(+) CD25(+) GITR(+) T regulatory cells found to express FoxP3 were isolated and transferred into mice prior to immunisation with IRBP. The immune response was scored by histopathology. Tregs were characterized in vitro by intracellular staining, cytokine secretion assay and transwell experiments.eDC secrete IL-10 but no IL-12 or IFN gamma. When injected subcutaneously into naive mice, they expand the population of CD4(+) CD25(+high) GITR(+) T cells expressing FoxP3 in the dLN, thus increasing the total number of IL-10 producing cells. eDC induced Tregs inhibit CD4(+) CD25(-) T effector cell proliferation by a contact dependent process, and both eDC and Tregs suppress retinal damage when adoptively transferred.We suggest that DC maturation may be necessary for both tolerance and immunity, but differential levels of activation and/or cytokine production direct the outcome of DC-T cell interaction and this is determined by IL-12 production. T regulatory cells induced in vivo by contact with eDC are able to suppress disease in the EAU model by adoptive transfer.

AB - Tolerance-inducing DC are considered to be less mature than immunogenic DC, but the conditions promoting a less mature DC phenotype are not clear. We have previously shown that lipopolysaccharide (LPS) can have differential effects on DC function depending on the timing of DC exposure to LPS. Here, we show that early LPS-activated bone marrow derived DC (early DC, eDC), when administered subcutaneously to mice in vivo, promote tolerance to EAU induced via immunisation with interphotoreceptor retinol binding protein (IRBP) peptide 161-180. The effect correlates with the failure of eDC to secrete IL-12, and appears to be mediated in part via expansion of naturally occurring CD4(+) CD25(+) T regulatory cells (Tregs), which also mediate suppression of EAU on adoptive transfer to naive mice followed by immunization with autoantigen.Immature DC were prepared from BMDC cultures. Early DC (eDC) and late DC (lDC) for tolerance experiments were obtained by differential timing of LPS addition and their cytokine secretion profile was analyzed. eDC and lDC were subcutaneously injected into mice. From the dLN CD4(+) CD25(+) GITR(+) T regulatory cells found to express FoxP3 were isolated and transferred into mice prior to immunisation with IRBP. The immune response was scored by histopathology. Tregs were characterized in vitro by intracellular staining, cytokine secretion assay and transwell experiments.eDC secrete IL-10 but no IL-12 or IFN gamma. When injected subcutaneously into naive mice, they expand the population of CD4(+) CD25(+high) GITR(+) T cells expressing FoxP3 in the dLN, thus increasing the total number of IL-10 producing cells. eDC induced Tregs inhibit CD4(+) CD25(-) T effector cell proliferation by a contact dependent process, and both eDC and Tregs suppress retinal damage when adoptively transferred.We suggest that DC maturation may be necessary for both tolerance and immunity, but differential levels of activation and/or cytokine production direct the outcome of DC-T cell interaction and this is determined by IL-12 production. T regulatory cells induced in vivo by contact with eDC are able to suppress disease in the EAU model by adoptive transfer.

KW - dendritic cells

KW - experimental autoimmune uveoretinitis

KW - lipopolysaccharide

KW - T regulatory cells

KW - toll-like receptors

KW - signaling pathway

KW - immune-responses

KW - IL-12 production

KW - GM-CSF

KW - tolerance

KW - induction

KW - mice

KW - inhibition

KW - molecule

U2 - 10.1007/s00417-006-0356-9

DO - 10.1007/s00417-006-0356-9

M3 - Article

VL - 245

SP - 221

EP - 229

JO - Graefe's Archive for Clinical and Experimental Ophthalmology

JF - Graefe's Archive for Clinical and Experimental Ophthalmology

SN - 0721-832X

IS - 2

ER -