Cell density-regulated recovery of starved biofilm populations of ammonia-oxidizing bacteria

S E Batchelor, Michael Cooper, S R Chhabra, Lesley Anne Glover, Brian Gray Stewart, Patricia Williams, James Ivor Prosser

Research output: Contribution to journalArticle

129 Citations (Scopus)

Abstract

The speed of recovery of cell suspensions and biofilm populations of the ammonia oxidizer Nitrosomonas europaea, following starvation was determined. Stationary-phase cells, washed and resuspended in ammonium-free inorganic medium, were starved for periods of up to 42 days, after which the medium was supplemented with ammonium and subsequent growth was monitored by measuring nitrite concentration changes. Cultures exhibited a lag phase prior to exponential nitrite production, which increased from 8.72 h (no starvation) to 153 h after starvation for 42 days. Biofilm populations of N. europaea colonizing sand or soil particles in continuous-flow, fixed column reactors were starved by continuous supply of ammonium-free medium. Following resupply of ammonium, starved biofilms exhibited no lag phase prior to nitrite production, even after starvation for 43.2 days, although there was evidence of cell loss during starvation. Biofilm formation will therefore provide a significant ecological advantage for ammonia oxidizers in natural environments in which the substrate supply is intermittent, Cell density-dependent phenomena in a number of gram-negative bacteria are mediated by N-acyl homoserine lactones (AHL), including N- (3-oxohexanoyl)-L-homoserine lactone (OHHL). Addition of both ammonium and OHHL to cell suspensions starved for 28 days decreased the lag phase in a concentration-dependent manner from 53.4 h to a minimum of 10.8 h, AHL production by N. europaea was detected by using a luxR-luxAB AHL reporter system. The results suggest that rapid recovery of high-density biofilm populations may be due to production and accumulation of OHHL to levels not possible in relatively low-density cell suspensions.

Original languageEnglish
Pages (from-to)2281-2286
Number of pages6
JournalApplied and Environmental Microbiology
Volume63
Issue number6
Publication statusPublished - Jun 1997

Keywords

  • ERWINIA-CAROTOVORA
  • NITROSOMONAS-EUROPAEA
  • N-(3-OXOHEXANOYL)-L-HOMOSERINE LACTONE
  • PSEUDOMONAS-AERUGINOSA
  • NITRIFYING BACTERIA
  • LUX AUTOINDUCER
  • GROWTH
  • BIOSYNTHESIS
  • NITRAPYRIN
  • INHIBITION

Cite this

Batchelor, S. E., Cooper, M., Chhabra, S. R., Glover, L. A., Stewart, B. G., Williams, P., & Prosser, J. I. (1997). Cell density-regulated recovery of starved biofilm populations of ammonia-oxidizing bacteria. Applied and Environmental Microbiology, 63(6), 2281-2286.

Cell density-regulated recovery of starved biofilm populations of ammonia-oxidizing bacteria. / Batchelor, S E ; Cooper, Michael; Chhabra, S R ; Glover, Lesley Anne; Stewart, Brian Gray; Williams, Patricia; Prosser, James Ivor.

In: Applied and Environmental Microbiology, Vol. 63, No. 6, 06.1997, p. 2281-2286.

Research output: Contribution to journalArticle

Batchelor, SE, Cooper, M, Chhabra, SR, Glover, LA, Stewart, BG, Williams, P & Prosser, JI 1997, 'Cell density-regulated recovery of starved biofilm populations of ammonia-oxidizing bacteria', Applied and Environmental Microbiology, vol. 63, no. 6, pp. 2281-2286.
Batchelor SE, Cooper M, Chhabra SR, Glover LA, Stewart BG, Williams P et al. Cell density-regulated recovery of starved biofilm populations of ammonia-oxidizing bacteria. Applied and Environmental Microbiology. 1997 Jun;63(6):2281-2286.
Batchelor, S E ; Cooper, Michael ; Chhabra, S R ; Glover, Lesley Anne ; Stewart, Brian Gray ; Williams, Patricia ; Prosser, James Ivor. / Cell density-regulated recovery of starved biofilm populations of ammonia-oxidizing bacteria. In: Applied and Environmental Microbiology. 1997 ; Vol. 63, No. 6. pp. 2281-2286.
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abstract = "The speed of recovery of cell suspensions and biofilm populations of the ammonia oxidizer Nitrosomonas europaea, following starvation was determined. Stationary-phase cells, washed and resuspended in ammonium-free inorganic medium, were starved for periods of up to 42 days, after which the medium was supplemented with ammonium and subsequent growth was monitored by measuring nitrite concentration changes. Cultures exhibited a lag phase prior to exponential nitrite production, which increased from 8.72 h (no starvation) to 153 h after starvation for 42 days. Biofilm populations of N. europaea colonizing sand or soil particles in continuous-flow, fixed column reactors were starved by continuous supply of ammonium-free medium. Following resupply of ammonium, starved biofilms exhibited no lag phase prior to nitrite production, even after starvation for 43.2 days, although there was evidence of cell loss during starvation. Biofilm formation will therefore provide a significant ecological advantage for ammonia oxidizers in natural environments in which the substrate supply is intermittent, Cell density-dependent phenomena in a number of gram-negative bacteria are mediated by N-acyl homoserine lactones (AHL), including N- (3-oxohexanoyl)-L-homoserine lactone (OHHL). Addition of both ammonium and OHHL to cell suspensions starved for 28 days decreased the lag phase in a concentration-dependent manner from 53.4 h to a minimum of 10.8 h, AHL production by N. europaea was detected by using a luxR-luxAB AHL reporter system. The results suggest that rapid recovery of high-density biofilm populations may be due to production and accumulation of OHHL to levels not possible in relatively low-density cell suspensions.",
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T1 - Cell density-regulated recovery of starved biofilm populations of ammonia-oxidizing bacteria

AU - Batchelor, S E

AU - Cooper, Michael

AU - Chhabra, S R

AU - Glover, Lesley Anne

AU - Stewart, Brian Gray

AU - Williams, Patricia

AU - Prosser, James Ivor

PY - 1997/6

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N2 - The speed of recovery of cell suspensions and biofilm populations of the ammonia oxidizer Nitrosomonas europaea, following starvation was determined. Stationary-phase cells, washed and resuspended in ammonium-free inorganic medium, were starved for periods of up to 42 days, after which the medium was supplemented with ammonium and subsequent growth was monitored by measuring nitrite concentration changes. Cultures exhibited a lag phase prior to exponential nitrite production, which increased from 8.72 h (no starvation) to 153 h after starvation for 42 days. Biofilm populations of N. europaea colonizing sand or soil particles in continuous-flow, fixed column reactors were starved by continuous supply of ammonium-free medium. Following resupply of ammonium, starved biofilms exhibited no lag phase prior to nitrite production, even after starvation for 43.2 days, although there was evidence of cell loss during starvation. Biofilm formation will therefore provide a significant ecological advantage for ammonia oxidizers in natural environments in which the substrate supply is intermittent, Cell density-dependent phenomena in a number of gram-negative bacteria are mediated by N-acyl homoserine lactones (AHL), including N- (3-oxohexanoyl)-L-homoserine lactone (OHHL). Addition of both ammonium and OHHL to cell suspensions starved for 28 days decreased the lag phase in a concentration-dependent manner from 53.4 h to a minimum of 10.8 h, AHL production by N. europaea was detected by using a luxR-luxAB AHL reporter system. The results suggest that rapid recovery of high-density biofilm populations may be due to production and accumulation of OHHL to levels not possible in relatively low-density cell suspensions.

AB - The speed of recovery of cell suspensions and biofilm populations of the ammonia oxidizer Nitrosomonas europaea, following starvation was determined. Stationary-phase cells, washed and resuspended in ammonium-free inorganic medium, were starved for periods of up to 42 days, after which the medium was supplemented with ammonium and subsequent growth was monitored by measuring nitrite concentration changes. Cultures exhibited a lag phase prior to exponential nitrite production, which increased from 8.72 h (no starvation) to 153 h after starvation for 42 days. Biofilm populations of N. europaea colonizing sand or soil particles in continuous-flow, fixed column reactors were starved by continuous supply of ammonium-free medium. Following resupply of ammonium, starved biofilms exhibited no lag phase prior to nitrite production, even after starvation for 43.2 days, although there was evidence of cell loss during starvation. Biofilm formation will therefore provide a significant ecological advantage for ammonia oxidizers in natural environments in which the substrate supply is intermittent, Cell density-dependent phenomena in a number of gram-negative bacteria are mediated by N-acyl homoserine lactones (AHL), including N- (3-oxohexanoyl)-L-homoserine lactone (OHHL). Addition of both ammonium and OHHL to cell suspensions starved for 28 days decreased the lag phase in a concentration-dependent manner from 53.4 h to a minimum of 10.8 h, AHL production by N. europaea was detected by using a luxR-luxAB AHL reporter system. The results suggest that rapid recovery of high-density biofilm populations may be due to production and accumulation of OHHL to levels not possible in relatively low-density cell suspensions.

KW - ERWINIA-CAROTOVORA

KW - NITROSOMONAS-EUROPAEA

KW - N-(3-OXOHEXANOYL)-L-HOMOSERINE LACTONE

KW - PSEUDOMONAS-AERUGINOSA

KW - NITRIFYING BACTERIA

KW - LUX AUTOINDUCER

KW - GROWTH

KW - BIOSYNTHESIS

KW - NITRAPYRIN

KW - INHIBITION

M3 - Article

VL - 63

SP - 2281

EP - 2286

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 6

ER -