Cell viability and laminin-induced neurite outgrowth in cultures of embryonic chick neural tube cells: effects of cytosine-B-D-arabinofuranoside

S H Parson, J F Price, R R Ribchester

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5 Citations (Scopus)

Abstract

We have measured the effects of cytosine-beta-D-arabinofuranoside (AraC) on cell survival and neurite outgrowth in cultures of dissociated 4-6 day embryonic chick neural tube cells. High concentrations of AraC (greater than 100 microM) reduced neuronal cell survival and neurite outgrowth from viable cells. Concentrations normally used to inhibit mitotic cell division (1-10 microM) were toxic to the neurones cultured in serum free medium on a poly-DL-ornithine/laminin substrate. AraC does not appear to have a neurite promoting effect on dissociated neurones that are cultured in the presence of low numbers of non-neuronal cells. This suggests that the neurite promoting effects of AraC reported by others is likely to be through the non-neuronal cells that were an inherent feature of the culturing systems in these studies. AraC cytotoxicity was completely blocked by the addition of the competitive antagonist: 2'deoxycytidine (2'DC) but not by its metabolic precursor cytosine (cyt). We suggest that the acute effects of AraC on neurones which are actively growing neurites are the result of interference with lipid metabolism.

Original languageEnglish
Pages (from-to)99-106
Number of pages8
JournalNeurodegeneration : a journal for neurodegenerative disorders, neuroprotection, and neuroregeneration
Volume4
Issue number1
Publication statusPublished - Mar 1995

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Neural Tube
Cytosine
Laminin
Cell Survival
Neurites
Neurons
Deoxycytidine
Ornithine
Poisons
Serum-Free Culture Media
Lipid Metabolism
Cell Division
Neuronal Outgrowth

Keywords

  • Animals
  • Biological Assay
  • Cell Survival
  • Cells, Cultured
  • Central Nervous System
  • Chick Embryo
  • Cytarabine
  • Cytosine
  • Deoxycytidine
  • Laminin
  • Neurites
  • Neurons

Cite this

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title = "Cell viability and laminin-induced neurite outgrowth in cultures of embryonic chick neural tube cells: effects of cytosine-B-D-arabinofuranoside",
abstract = "We have measured the effects of cytosine-beta-D-arabinofuranoside (AraC) on cell survival and neurite outgrowth in cultures of dissociated 4-6 day embryonic chick neural tube cells. High concentrations of AraC (greater than 100 microM) reduced neuronal cell survival and neurite outgrowth from viable cells. Concentrations normally used to inhibit mitotic cell division (1-10 microM) were toxic to the neurones cultured in serum free medium on a poly-DL-ornithine/laminin substrate. AraC does not appear to have a neurite promoting effect on dissociated neurones that are cultured in the presence of low numbers of non-neuronal cells. This suggests that the neurite promoting effects of AraC reported by others is likely to be through the non-neuronal cells that were an inherent feature of the culturing systems in these studies. AraC cytotoxicity was completely blocked by the addition of the competitive antagonist: 2'deoxycytidine (2'DC) but not by its metabolic precursor cytosine (cyt). We suggest that the acute effects of AraC on neurones which are actively growing neurites are the result of interference with lipid metabolism.",
keywords = "Animals, Biological Assay, Cell Survival, Cells, Cultured, Central Nervous System, Chick Embryo, Cytarabine, Cytosine, Deoxycytidine, Laminin, Neurites, Neurons",
author = "Parson, {S H} and Price, {J F} and Ribchester, {R R}",
year = "1995",
month = "3",
language = "English",
volume = "4",
pages = "99--106",
journal = "Neurodegeneration : a journal for neurodegenerative disorders, neuroprotection, and neuroregeneration",
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TY - JOUR

T1 - Cell viability and laminin-induced neurite outgrowth in cultures of embryonic chick neural tube cells

T2 - effects of cytosine-B-D-arabinofuranoside

AU - Parson, S H

AU - Price, J F

AU - Ribchester, R R

PY - 1995/3

Y1 - 1995/3

N2 - We have measured the effects of cytosine-beta-D-arabinofuranoside (AraC) on cell survival and neurite outgrowth in cultures of dissociated 4-6 day embryonic chick neural tube cells. High concentrations of AraC (greater than 100 microM) reduced neuronal cell survival and neurite outgrowth from viable cells. Concentrations normally used to inhibit mitotic cell division (1-10 microM) were toxic to the neurones cultured in serum free medium on a poly-DL-ornithine/laminin substrate. AraC does not appear to have a neurite promoting effect on dissociated neurones that are cultured in the presence of low numbers of non-neuronal cells. This suggests that the neurite promoting effects of AraC reported by others is likely to be through the non-neuronal cells that were an inherent feature of the culturing systems in these studies. AraC cytotoxicity was completely blocked by the addition of the competitive antagonist: 2'deoxycytidine (2'DC) but not by its metabolic precursor cytosine (cyt). We suggest that the acute effects of AraC on neurones which are actively growing neurites are the result of interference with lipid metabolism.

AB - We have measured the effects of cytosine-beta-D-arabinofuranoside (AraC) on cell survival and neurite outgrowth in cultures of dissociated 4-6 day embryonic chick neural tube cells. High concentrations of AraC (greater than 100 microM) reduced neuronal cell survival and neurite outgrowth from viable cells. Concentrations normally used to inhibit mitotic cell division (1-10 microM) were toxic to the neurones cultured in serum free medium on a poly-DL-ornithine/laminin substrate. AraC does not appear to have a neurite promoting effect on dissociated neurones that are cultured in the presence of low numbers of non-neuronal cells. This suggests that the neurite promoting effects of AraC reported by others is likely to be through the non-neuronal cells that were an inherent feature of the culturing systems in these studies. AraC cytotoxicity was completely blocked by the addition of the competitive antagonist: 2'deoxycytidine (2'DC) but not by its metabolic precursor cytosine (cyt). We suggest that the acute effects of AraC on neurones which are actively growing neurites are the result of interference with lipid metabolism.

KW - Animals

KW - Biological Assay

KW - Cell Survival

KW - Cells, Cultured

KW - Central Nervous System

KW - Chick Embryo

KW - Cytarabine

KW - Cytosine

KW - Deoxycytidine

KW - Laminin

KW - Neurites

KW - Neurons

M3 - Article

C2 - 7600190

VL - 4

SP - 99

EP - 106

JO - Neurodegeneration : a journal for neurodegenerative disorders, neuroprotection, and neuroregeneration

JF - Neurodegeneration : a journal for neurodegenerative disorders, neuroprotection, and neuroregeneration

SN - 1055-8330

IS - 1

ER -