Cellular interactions of Candida albicans with human oral epithelial cells and enterocytes

Frederic Dalle, Betty Wächtler, Coralie L'Ollivier, Gudrun Holland, Norbert Bannert, Duncan Wilson, Catherine Labruère, Alain Bonnin, Bernhard Hube

Research output: Contribution to journalArticle

186 Citations (Scopus)

Abstract

The human pathogenic fungus Candida albicans can cause systemic infections by invading epithelial barriers to gain access to the bloodstream. One of the main reservoirs of C. albicans is the gastrointestinal tract and systemic infections predominantly originate from this niche. In this study, we used scanning electron and fluorescence microscopy, adhesion, invasion and damage assays, fungal mutants and a set of fungal and host cell inhibitors to investigate the interactions of C. albicans with oral epithelial cells and enterocytes. Our data demonstrate that adhesion, invasion and damage by C. albicans depend not only on fungal morphology and activity, but also on the epithelial cell type and the differentiation stage of the epithelial cells, indicating that epithelial cells differ in their susceptibility to the fungus. C. albicans can invade epithelial cells by induced endocytosis and/or active penetration. However, depending on the host cell faced by the fungus, these routes are exploited to a different extent. While invasion into oral cells occurs via both routes, invasion into intestinal cells occurs only via active penetration.
Original languageEnglish
Pages (from-to)248-271
Number of pages24
JournalCellular Microbiology
Volume12
Issue number2
Early online date27 Oct 2009
DOIs
Publication statusPublished - Feb 2010

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Enterocytes
Candida albicans
Epithelial Cells
Fungi
Endocytosis
Infection
Fluorescence Microscopy
Gastrointestinal Tract
Cell Differentiation
Electron Microscopy

Keywords

  • CaCo-2 cells
  • Candida albicans
  • cell differentiation
  • cell line, tumor
  • enterocytes
  • epithelial cells
  • host-pathogen interactions
  • humans
  • microscopy, electron, scanning
  • microscopy, fluorescence

Cite this

Dalle, F., Wächtler, B., L'Ollivier, C., Holland, G., Bannert, N., Wilson, D., ... Hube, B. (2010). Cellular interactions of Candida albicans with human oral epithelial cells and enterocytes. Cellular Microbiology, 12(2), 248-271. https://doi.org/10.1111/j.1462-5822.2009.01394.x

Cellular interactions of Candida albicans with human oral epithelial cells and enterocytes. / Dalle, Frederic; Wächtler, Betty; L'Ollivier, Coralie; Holland, Gudrun; Bannert, Norbert; Wilson, Duncan; Labruère, Catherine; Bonnin, Alain; Hube, Bernhard.

In: Cellular Microbiology, Vol. 12, No. 2, 02.2010, p. 248-271.

Research output: Contribution to journalArticle

Dalle, F, Wächtler, B, L'Ollivier, C, Holland, G, Bannert, N, Wilson, D, Labruère, C, Bonnin, A & Hube, B 2010, 'Cellular interactions of Candida albicans with human oral epithelial cells and enterocytes', Cellular Microbiology, vol. 12, no. 2, pp. 248-271. https://doi.org/10.1111/j.1462-5822.2009.01394.x
Dalle, Frederic ; Wächtler, Betty ; L'Ollivier, Coralie ; Holland, Gudrun ; Bannert, Norbert ; Wilson, Duncan ; Labruère, Catherine ; Bonnin, Alain ; Hube, Bernhard. / Cellular interactions of Candida albicans with human oral epithelial cells and enterocytes. In: Cellular Microbiology. 2010 ; Vol. 12, No. 2. pp. 248-271.
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