Cellular Zn depletion by metal ion chelators (TPEN, DTPA and chelex resin) and its application to osteoblastic MC3T3-E1 cells

Young-Eun Cho, Ria-Ann R Lomeda, Sang-Hoon Ryu, Jong-Hwa Lee, John H Beattie, In-Sook Kwun

    Research output: Contribution to journalArticle

    Abstract

    Trace mineral studies involving metal ion chelators have been conducted in investigating the response of gene and protein expressions of certain cell lines but a few had really focused on how these metal ion chelators could affect the availability of important trace minerals such as Zn, Mn, Fe and Cu. The aim of the present study was to investigate the availability of Zn for the treatment of MC3T3-E1 osteoblast-like cells and the availability of some trace minerals in the cell culture media components after using chelexing resin in the FBS and the addition of N,N,N',N'-tetrakis-(2-pyridylmethyl)ethylenediamine (TPEN, membrane-permeable chelator) and diethylenetriaminepentaacetic acid (DTPA, membrane-impermeable chelator) in the treatment medium. Components for the preparation of cell culture medium and Zn-treated medium have been tested for Zn, Mn, Fe and Cu contents by atomic absorption spectrophotometer or inductively coupled plasma spectrophotometer. Also, the expression of bone-related genes (ALP, Runx2, PTH-R, ProCOL I, OPN and OC) was measured on the cellular Zn depletion such as chelexing or TPEN treatment. Results have shown that using the chelexing resin in FBS would significantly decrease the available Zn (p
    Original languageEnglish
    Pages (from-to)29-35
    Number of pages7
    JournalNutrition Research and Practice
    Volume1
    Issue number1
    DOIs
    Publication statusPublished - 2007

    Fingerprint

    Pentetic Acid
    Chelating Agents
    chelating agents
    metal ions
    resins
    Trace Elements
    Metals
    Ions
    trace elements
    spectrophotometers
    Culture Media
    cell culture
    Cell Culture Techniques
    culture media
    cells
    Membranes
    osteoblasts
    Osteoblasts
    protein synthesis
    cell lines

    Keywords

    • Zn depletion
    • metal ion chelators
    • DTPA
    • TPEN
    • chelexing
    • MC3T-E1 cells

    Cite this

    Cellular Zn depletion by metal ion chelators (TPEN, DTPA and chelex resin) and its application to osteoblastic MC3T3-E1 cells. / Cho, Young-Eun; Lomeda, Ria-Ann R; Ryu, Sang-Hoon; Lee, Jong-Hwa; Beattie, John H; Kwun, In-Sook.

    In: Nutrition Research and Practice, Vol. 1, No. 1, 2007, p. 29-35.

    Research output: Contribution to journalArticle

    Cho, Young-Eun ; Lomeda, Ria-Ann R ; Ryu, Sang-Hoon ; Lee, Jong-Hwa ; Beattie, John H ; Kwun, In-Sook. / Cellular Zn depletion by metal ion chelators (TPEN, DTPA and chelex resin) and its application to osteoblastic MC3T3-E1 cells. In: Nutrition Research and Practice. 2007 ; Vol. 1, No. 1. pp. 29-35.
    @article{ed420923147545519b8f369cf44ce1b0,
    title = "Cellular Zn depletion by metal ion chelators (TPEN, DTPA and chelex resin) and its application to osteoblastic MC3T3-E1 cells",
    abstract = "Trace mineral studies involving metal ion chelators have been conducted in investigating the response of gene and protein expressions of certain cell lines but a few had really focused on how these metal ion chelators could affect the availability of important trace minerals such as Zn, Mn, Fe and Cu. The aim of the present study was to investigate the availability of Zn for the treatment of MC3T3-E1 osteoblast-like cells and the availability of some trace minerals in the cell culture media components after using chelexing resin in the FBS and the addition of N,N,N',N'-tetrakis-(2-pyridylmethyl)ethylenediamine (TPEN, membrane-permeable chelator) and diethylenetriaminepentaacetic acid (DTPA, membrane-impermeable chelator) in the treatment medium. Components for the preparation of cell culture medium and Zn-treated medium have been tested for Zn, Mn, Fe and Cu contents by atomic absorption spectrophotometer or inductively coupled plasma spectrophotometer. Also, the expression of bone-related genes (ALP, Runx2, PTH-R, ProCOL I, OPN and OC) was measured on the cellular Zn depletion such as chelexing or TPEN treatment. Results have shown that using the chelexing resin in FBS would significantly decrease the available Zn (p",
    keywords = "Zn depletion, metal ion chelators, DTPA, TPEN, chelexing, MC3T-E1 cells",
    author = "Young-Eun Cho and Lomeda, {Ria-Ann R} and Sang-Hoon Ryu and Jong-Hwa Lee and Beattie, {John H} and In-Sook Kwun",
    year = "2007",
    doi = "10.4162/nrp.2007.1.1.29",
    language = "English",
    volume = "1",
    pages = "29--35",
    journal = "Nutrition Research and Practice",
    issn = "1976-1457",
    publisher = "The Korean Society of Community Nutrition",
    number = "1",

    }

    TY - JOUR

    T1 - Cellular Zn depletion by metal ion chelators (TPEN, DTPA and chelex resin) and its application to osteoblastic MC3T3-E1 cells

    AU - Cho, Young-Eun

    AU - Lomeda, Ria-Ann R

    AU - Ryu, Sang-Hoon

    AU - Lee, Jong-Hwa

    AU - Beattie, John H

    AU - Kwun, In-Sook

    PY - 2007

    Y1 - 2007

    N2 - Trace mineral studies involving metal ion chelators have been conducted in investigating the response of gene and protein expressions of certain cell lines but a few had really focused on how these metal ion chelators could affect the availability of important trace minerals such as Zn, Mn, Fe and Cu. The aim of the present study was to investigate the availability of Zn for the treatment of MC3T3-E1 osteoblast-like cells and the availability of some trace minerals in the cell culture media components after using chelexing resin in the FBS and the addition of N,N,N',N'-tetrakis-(2-pyridylmethyl)ethylenediamine (TPEN, membrane-permeable chelator) and diethylenetriaminepentaacetic acid (DTPA, membrane-impermeable chelator) in the treatment medium. Components for the preparation of cell culture medium and Zn-treated medium have been tested for Zn, Mn, Fe and Cu contents by atomic absorption spectrophotometer or inductively coupled plasma spectrophotometer. Also, the expression of bone-related genes (ALP, Runx2, PTH-R, ProCOL I, OPN and OC) was measured on the cellular Zn depletion such as chelexing or TPEN treatment. Results have shown that using the chelexing resin in FBS would significantly decrease the available Zn (p

    AB - Trace mineral studies involving metal ion chelators have been conducted in investigating the response of gene and protein expressions of certain cell lines but a few had really focused on how these metal ion chelators could affect the availability of important trace minerals such as Zn, Mn, Fe and Cu. The aim of the present study was to investigate the availability of Zn for the treatment of MC3T3-E1 osteoblast-like cells and the availability of some trace minerals in the cell culture media components after using chelexing resin in the FBS and the addition of N,N,N',N'-tetrakis-(2-pyridylmethyl)ethylenediamine (TPEN, membrane-permeable chelator) and diethylenetriaminepentaacetic acid (DTPA, membrane-impermeable chelator) in the treatment medium. Components for the preparation of cell culture medium and Zn-treated medium have been tested for Zn, Mn, Fe and Cu contents by atomic absorption spectrophotometer or inductively coupled plasma spectrophotometer. Also, the expression of bone-related genes (ALP, Runx2, PTH-R, ProCOL I, OPN and OC) was measured on the cellular Zn depletion such as chelexing or TPEN treatment. Results have shown that using the chelexing resin in FBS would significantly decrease the available Zn (p

    KW - Zn depletion

    KW - metal ion chelators

    KW - DTPA

    KW - TPEN

    KW - chelexing

    KW - MC3T-E1 cells

    U2 - 10.4162/nrp.2007.1.1.29

    DO - 10.4162/nrp.2007.1.1.29

    M3 - Article

    VL - 1

    SP - 29

    EP - 35

    JO - Nutrition Research and Practice

    JF - Nutrition Research and Practice

    SN - 1976-1457

    IS - 1

    ER -