TY - JOUR
T1 - Census of bacterial microbiota associated with the glacier ice worm Mesenchytraeus solifugus
AU - Murakami, Takumi
AU - Segawa, Takahiro
AU - Bodington, Dylan
AU - Dial, Roman
AU - Takeuchi, Nozomu
AU - Kohshima, Shiro
AU - Hongoh, Yuichi
N1 - FUNDING
This study was financially supported by the Grant-in-Aid for Scientific Research from the Japan Society for Promotion of Science to S.K (22241005 and 26241020) and Y.H. (23117003) and the NEXT program to Y.H.
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PY - 2015/1/12
Y1 - 2015/1/12
N2 - The glacier ice worm, Mesenchytraeus solifugus, is a unique annelid, inhabiting only snow and ice in North American glaciers. Here, we analyzed the taxonomic composition of bacteria associated with M. solifugus based on the 16S rRNA gene. We analyzed four fixed-on-site and 10 starved ice worm individuals, along with glacier surface samples. In total, 1341 clones of 16S rRNA genes were analyzed for the ice worm samples, from which 65 bacterial phylotypes (99.0% cut-off) were identified. Of these, 35 phylotypes were closely related to sequences obtained from their habitat glacier and/or other components of cryosphere; whereas three dominant phylotypes were affiliated with animal-associated lineages of the class Mollicutes. Among the three, phylotype Ms-13 shared less than 89% similarity with database sequences and was closest to a gut symbiont of a terrestrial earthworm. Using fluorescence in situ hybridization, Ms-13 was located on the gut wall surface of the ice worms. We propose a novel genus and species, 'Candidatus Vermiplasma glacialis', for this bacterium. Our results raise the possibility that the ice worm has exploited indigenous glacier bacteria, while several symbiotic bacterial lineages have maintained their association with the ice worm during the course of adaptive evolution to the permanently cold environment.
AB - The glacier ice worm, Mesenchytraeus solifugus, is a unique annelid, inhabiting only snow and ice in North American glaciers. Here, we analyzed the taxonomic composition of bacteria associated with M. solifugus based on the 16S rRNA gene. We analyzed four fixed-on-site and 10 starved ice worm individuals, along with glacier surface samples. In total, 1341 clones of 16S rRNA genes were analyzed for the ice worm samples, from which 65 bacterial phylotypes (99.0% cut-off) were identified. Of these, 35 phylotypes were closely related to sequences obtained from their habitat glacier and/or other components of cryosphere; whereas three dominant phylotypes were affiliated with animal-associated lineages of the class Mollicutes. Among the three, phylotype Ms-13 shared less than 89% similarity with database sequences and was closest to a gut symbiont of a terrestrial earthworm. Using fluorescence in situ hybridization, Ms-13 was located on the gut wall surface of the ice worms. We propose a novel genus and species, 'Candidatus Vermiplasma glacialis', for this bacterium. Our results raise the possibility that the ice worm has exploited indigenous glacier bacteria, while several symbiotic bacterial lineages have maintained their association with the ice worm during the course of adaptive evolution to the permanently cold environment.
KW - Animals
KW - Annelida/microbiology
KW - Base Sequence
KW - DNA, Bacterial/genetics
KW - Gastrointestinal Tract/microbiology
KW - Ice Cover/microbiology
KW - In Situ Hybridization, Fluorescence
KW - Microbiota/genetics
KW - Molecular Sequence Data
KW - Phylogeny
KW - RNA, Ribosomal, 16S/genetics
KW - Sequence Analysis, DNA
KW - Tenericutes/classification
U2 - 10.1093/femsec/fiv003
DO - 10.1093/femsec/fiv003
M3 - Article
C2 - 25764456
VL - 91
JO - FEMS Microbiology Ecology
JF - FEMS Microbiology Ecology
SN - 1574-6941
IS - 3
M1 - fiv003
ER -