Cerebellar granule cell Cre recombinase expression.

M. I. Aller, A. Jones, D. Merlo, M. Paterlini, A. H. Meyer, U. Amtmann, S. G. Brickley, H. E. Jolin, A. N. J. McKenzie, H. Monyer, M. Farrant, William Wisden

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    36 Citations (Scopus)

    Abstract

    The cerebellum maintains balance and orientation, refines motor action, stores motor memories, and contributes to the timing aspects of cognition. We generated two mouse lines for making Cre recombinase-mediated gene disruptions largely confined to adult cerebellar granule cells. For this purpose we chose the GABA(A) receptor alpha6 subunit gene, whose expression marks this cell type. Here we describe mouse lines expressing Cre recombinase generated by 1) Cre knocked into the native A subunit gene by homologous recombination in embryonic stem cells; and 2) Cre recombined into an alpha6 subunit gene carried on a bacterial artificial chromosome (BAC) genomic clone. The fidelity of Cre expression was tested by crossing the mouse lines with the ROSA26 reporter mice. The particular alpha6BAC clone we identified will be valuable for delivering other gene products to cerebellar granule cells. (C) 2003 Wiley-Liss, Inc.

    Original languageEnglish
    Pages (from-to)97-103
    Number of pages6
    JournalGenesis
    Volume36
    DOIs
    Publication statusPublished - 2003

    Keywords

    • Cre recombinase
    • BAC transgene
    • cerebellum
    • granule cell
    • GABA(A) receptor alpha 6 subunit gene
    • internal ribosome entry site
    • DIRECTING GENE-EXPRESSION
    • SUBUNIT MESSENGER-RNAS
    • ALPHA 6 SUBUNIT
    • TRANSGENIC MICE
    • RAT-BRAIN
    • RECEPTOR
    • DIFFERENTIATION

    Cite this

    Aller, M. I., Jones, A., Merlo, D., Paterlini, M., Meyer, A. H., Amtmann, U., Brickley, S. G., Jolin, H. E., McKenzie, A. N. J., Monyer, H., Farrant, M., & Wisden, W. (2003). Cerebellar granule cell Cre recombinase expression. Genesis, 36, 97-103. https://doi.org/10.1002/gene.10204