Changes in soil microbial biomass and microbial activities in response to 7 years simulated pollutant nitrogen deposition on a heathland and two grasslands

D Johnson, J R Leake, J A Lee, C D Campbell

Research output: Contribution to journalArticle

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Abstract

The effects of 7 years simulated pollutant nitrogen (N) deposition on soil microbial biomass C (C-mic), soil phosphomonoesterase (PME) activity and utilisation of carbon (C) and organic N and phosphorus (P) sources by soil bacteria are reported for a heathland, an acidic grassland and a calcareous grassland. N additions increased C-mic, in the heathland, decreased it in the acid grassland, and had no effect in the calcareous grassland. These effects mirrored the changes in plant cover, indicating close-coupling of plant and microbial responses to chronic N additions. In the heathland, PME activity generally rose with each increment of N addition while in the acid grassland, PME activity significantly increased only in the 14 g N m(-2) y(-1) (ammonium nitrate) treatment. In the calcareous grassland, PME activity was highly correlated with KCl extractable N (R-2 = 0.71), indicating increased PME activity in response to increasing N saturation. At all three sites, PME activity per mg C-mic,was greater in plots receiving N inputs, reflecting greater P limitation. In the heathland, N treatments caused a 3-foId increase in the utilisation rate of C and organic N substrates in BIOLOG plates, whereas utilisation of organic P substrates rose 10-fold in response to 8 g N m(-2) y(-1) and 18-fold in response to 12 g N m(-2) y(-1). In the acid grassland, utilisation of the C sources decreased in response to the N treatments. The results demonstrate that long-term chronic inputs of pollutant N can significantly increase microbial biomass and activity in N-limited heathland ecosystems, but may reduce microbial biomass and microbial activity in P-limited grasslands. (C) 1998 Elsevier Science Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)239-250
Number of pages12
JournalEnvironmental Pollution
Volume103
Issue number2-3
DOIs
Publication statusPublished - 1 Nov 1998

Keywords

  • nitrogen
  • nitrogen saturation
  • phosphase activity
  • soil microbial biomass
  • BIOLOG plates
  • acid-phosphatase-activity
  • diversicolor F Muell
  • forest soils
  • nutrient availability
  • organic phosphorus
  • calluna-vulgaris
  • ecosystems
  • litter
  • carbon
  • plant

Cite this

Changes in soil microbial biomass and microbial activities in response to 7 years simulated pollutant nitrogen deposition on a heathland and two grasslands. / Johnson, D ; Leake, J R ; Lee, J A ; Campbell, C D .

In: Environmental Pollution, Vol. 103, No. 2-3, 01.11.1998, p. 239-250.

Research output: Contribution to journalArticle

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N2 - The effects of 7 years simulated pollutant nitrogen (N) deposition on soil microbial biomass C (C-mic), soil phosphomonoesterase (PME) activity and utilisation of carbon (C) and organic N and phosphorus (P) sources by soil bacteria are reported for a heathland, an acidic grassland and a calcareous grassland. N additions increased C-mic, in the heathland, decreased it in the acid grassland, and had no effect in the calcareous grassland. These effects mirrored the changes in plant cover, indicating close-coupling of plant and microbial responses to chronic N additions. In the heathland, PME activity generally rose with each increment of N addition while in the acid grassland, PME activity significantly increased only in the 14 g N m(-2) y(-1) (ammonium nitrate) treatment. In the calcareous grassland, PME activity was highly correlated with KCl extractable N (R-2 = 0.71), indicating increased PME activity in response to increasing N saturation. At all three sites, PME activity per mg C-mic,was greater in plots receiving N inputs, reflecting greater P limitation. In the heathland, N treatments caused a 3-foId increase in the utilisation rate of C and organic N substrates in BIOLOG plates, whereas utilisation of organic P substrates rose 10-fold in response to 8 g N m(-2) y(-1) and 18-fold in response to 12 g N m(-2) y(-1). In the acid grassland, utilisation of the C sources decreased in response to the N treatments. The results demonstrate that long-term chronic inputs of pollutant N can significantly increase microbial biomass and activity in N-limited heathland ecosystems, but may reduce microbial biomass and microbial activity in P-limited grasslands. (C) 1998 Elsevier Science Ltd. All rights reserved.

AB - The effects of 7 years simulated pollutant nitrogen (N) deposition on soil microbial biomass C (C-mic), soil phosphomonoesterase (PME) activity and utilisation of carbon (C) and organic N and phosphorus (P) sources by soil bacteria are reported for a heathland, an acidic grassland and a calcareous grassland. N additions increased C-mic, in the heathland, decreased it in the acid grassland, and had no effect in the calcareous grassland. These effects mirrored the changes in plant cover, indicating close-coupling of plant and microbial responses to chronic N additions. In the heathland, PME activity generally rose with each increment of N addition while in the acid grassland, PME activity significantly increased only in the 14 g N m(-2) y(-1) (ammonium nitrate) treatment. In the calcareous grassland, PME activity was highly correlated with KCl extractable N (R-2 = 0.71), indicating increased PME activity in response to increasing N saturation. At all three sites, PME activity per mg C-mic,was greater in plots receiving N inputs, reflecting greater P limitation. In the heathland, N treatments caused a 3-foId increase in the utilisation rate of C and organic N substrates in BIOLOG plates, whereas utilisation of organic P substrates rose 10-fold in response to 8 g N m(-2) y(-1) and 18-fold in response to 12 g N m(-2) y(-1). In the acid grassland, utilisation of the C sources decreased in response to the N treatments. The results demonstrate that long-term chronic inputs of pollutant N can significantly increase microbial biomass and activity in N-limited heathland ecosystems, but may reduce microbial biomass and microbial activity in P-limited grasslands. (C) 1998 Elsevier Science Ltd. All rights reserved.

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KW - forest soils

KW - nutrient availability

KW - organic phosphorus

KW - calluna-vulgaris

KW - ecosystems

KW - litter

KW - carbon

KW - plant

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