Changes in tissue free amino acid concentrations in Atlantic salmon, Salmo salar L., after consumption of a low ration

C G Carter, D F Houlihan, Z Y He

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Atlantic salmon (Salmo salar L.) with an average weight of 411 +/- 16 g were fed after a period of 7 days without food and the free amino acid concentrations in the pylorus, liver and white muscle measured before and at 3, 6, 9, 15 and 24 h after feeding. There were few significant postprandial changes in tissue free amino acid concentrations. In the white muscle, concentrations of six indispensable amino acids were significantly (p < 0.05) higher after 9 (Ile, Leu, Phe, Thr) or 15 h (Val, Met). Individual feed intake was measured and there were significant positive correlations between amino acid intake and amino acid concentrations in white muscle free pools for total amino acids (p < 0.001), total indispensable amino acids (p < 0.001) and individual indispensable amino acids (Ile, Leu, Lys, Met, Phe, Val). These relationships were due to relatively low feed intake (0.28% body weight) that followed 7 days without feeding. The indispensable amino acid profile of the white muscle free pool was compared with that of standard proteins (the feed, whole body and white muscle), as well as with indispensable amino acids requirements. At different times one of two indispensable amino acids, Phe (at 0, 3, 6, 9, 15 h after feeding) or Trp (at 24 h after feeding), was present at the lowest relative concentrations compared to the other indispensable amino acids. This showed that although changes in tissue free amino acid concentrations following feeding were small the amino acid profile (relative concentrations) in the white muscle free pool changed. It is proposed that the lowest relative concentration of an indispensable amino acid in the white muscle free pool should be considered in relation to its potential to limit the efficiency of protein synthesis and retention.

Original languageEnglish
Pages (from-to)295-306
Number of pages12
JournalFish Physiology and Biochemistry
Volume23
Publication statusPublished - 2000

Keywords

  • Atlantic salmon
  • essential amino acids
  • feed intake
  • free amino acid pools
  • indispensable amino acids
  • RAINBOW-TROUT
  • POSTPRANDIAL CHANGES
  • CHANNEL CATFISH
  • WHOLE-BODY
  • FED DIETS
  • REQUIREMENT
  • PROTEIN
  • PLASMA
  • MUSCLE
  • GAIRDNERI

Cite this

Changes in tissue free amino acid concentrations in Atlantic salmon, Salmo salar L., after consumption of a low ration. / Carter, C G ; Houlihan, D F ; He, Z Y .

In: Fish Physiology and Biochemistry, Vol. 23, 2000, p. 295-306.

Research output: Contribution to journalArticle

@article{08939a52ad864167b69a3baf0607c4f5,
title = "Changes in tissue free amino acid concentrations in Atlantic salmon, Salmo salar L., after consumption of a low ration",
abstract = "Atlantic salmon (Salmo salar L.) with an average weight of 411 +/- 16 g were fed after a period of 7 days without food and the free amino acid concentrations in the pylorus, liver and white muscle measured before and at 3, 6, 9, 15 and 24 h after feeding. There were few significant postprandial changes in tissue free amino acid concentrations. In the white muscle, concentrations of six indispensable amino acids were significantly (p < 0.05) higher after 9 (Ile, Leu, Phe, Thr) or 15 h (Val, Met). Individual feed intake was measured and there were significant positive correlations between amino acid intake and amino acid concentrations in white muscle free pools for total amino acids (p < 0.001), total indispensable amino acids (p < 0.001) and individual indispensable amino acids (Ile, Leu, Lys, Met, Phe, Val). These relationships were due to relatively low feed intake (0.28{\%} body weight) that followed 7 days without feeding. The indispensable amino acid profile of the white muscle free pool was compared with that of standard proteins (the feed, whole body and white muscle), as well as with indispensable amino acids requirements. At different times one of two indispensable amino acids, Phe (at 0, 3, 6, 9, 15 h after feeding) or Trp (at 24 h after feeding), was present at the lowest relative concentrations compared to the other indispensable amino acids. This showed that although changes in tissue free amino acid concentrations following feeding were small the amino acid profile (relative concentrations) in the white muscle free pool changed. It is proposed that the lowest relative concentration of an indispensable amino acid in the white muscle free pool should be considered in relation to its potential to limit the efficiency of protein synthesis and retention.",
keywords = "Atlantic salmon, essential amino acids, feed intake, free amino acid pools, indispensable amino acids, RAINBOW-TROUT, POSTPRANDIAL CHANGES, CHANNEL CATFISH, WHOLE-BODY, FED DIETS, REQUIREMENT, PROTEIN, PLASMA, MUSCLE, GAIRDNERI",
author = "Carter, {C G} and Houlihan, {D F} and He, {Z Y}",
year = "2000",
language = "English",
volume = "23",
pages = "295--306",
journal = "Fish Physiology and Biochemistry",
issn = "0920-1742",
publisher = "Springer Netherlands",

}

TY - JOUR

T1 - Changes in tissue free amino acid concentrations in Atlantic salmon, Salmo salar L., after consumption of a low ration

AU - Carter, C G

AU - Houlihan, D F

AU - He, Z Y

PY - 2000

Y1 - 2000

N2 - Atlantic salmon (Salmo salar L.) with an average weight of 411 +/- 16 g were fed after a period of 7 days without food and the free amino acid concentrations in the pylorus, liver and white muscle measured before and at 3, 6, 9, 15 and 24 h after feeding. There were few significant postprandial changes in tissue free amino acid concentrations. In the white muscle, concentrations of six indispensable amino acids were significantly (p < 0.05) higher after 9 (Ile, Leu, Phe, Thr) or 15 h (Val, Met). Individual feed intake was measured and there were significant positive correlations between amino acid intake and amino acid concentrations in white muscle free pools for total amino acids (p < 0.001), total indispensable amino acids (p < 0.001) and individual indispensable amino acids (Ile, Leu, Lys, Met, Phe, Val). These relationships were due to relatively low feed intake (0.28% body weight) that followed 7 days without feeding. The indispensable amino acid profile of the white muscle free pool was compared with that of standard proteins (the feed, whole body and white muscle), as well as with indispensable amino acids requirements. At different times one of two indispensable amino acids, Phe (at 0, 3, 6, 9, 15 h after feeding) or Trp (at 24 h after feeding), was present at the lowest relative concentrations compared to the other indispensable amino acids. This showed that although changes in tissue free amino acid concentrations following feeding were small the amino acid profile (relative concentrations) in the white muscle free pool changed. It is proposed that the lowest relative concentration of an indispensable amino acid in the white muscle free pool should be considered in relation to its potential to limit the efficiency of protein synthesis and retention.

AB - Atlantic salmon (Salmo salar L.) with an average weight of 411 +/- 16 g were fed after a period of 7 days without food and the free amino acid concentrations in the pylorus, liver and white muscle measured before and at 3, 6, 9, 15 and 24 h after feeding. There were few significant postprandial changes in tissue free amino acid concentrations. In the white muscle, concentrations of six indispensable amino acids were significantly (p < 0.05) higher after 9 (Ile, Leu, Phe, Thr) or 15 h (Val, Met). Individual feed intake was measured and there were significant positive correlations between amino acid intake and amino acid concentrations in white muscle free pools for total amino acids (p < 0.001), total indispensable amino acids (p < 0.001) and individual indispensable amino acids (Ile, Leu, Lys, Met, Phe, Val). These relationships were due to relatively low feed intake (0.28% body weight) that followed 7 days without feeding. The indispensable amino acid profile of the white muscle free pool was compared with that of standard proteins (the feed, whole body and white muscle), as well as with indispensable amino acids requirements. At different times one of two indispensable amino acids, Phe (at 0, 3, 6, 9, 15 h after feeding) or Trp (at 24 h after feeding), was present at the lowest relative concentrations compared to the other indispensable amino acids. This showed that although changes in tissue free amino acid concentrations following feeding were small the amino acid profile (relative concentrations) in the white muscle free pool changed. It is proposed that the lowest relative concentration of an indispensable amino acid in the white muscle free pool should be considered in relation to its potential to limit the efficiency of protein synthesis and retention.

KW - Atlantic salmon

KW - essential amino acids

KW - feed intake

KW - free amino acid pools

KW - indispensable amino acids

KW - RAINBOW-TROUT

KW - POSTPRANDIAL CHANGES

KW - CHANNEL CATFISH

KW - WHOLE-BODY

KW - FED DIETS

KW - REQUIREMENT

KW - PROTEIN

KW - PLASMA

KW - MUSCLE

KW - GAIRDNERI

M3 - Article

VL - 23

SP - 295

EP - 306

JO - Fish Physiology and Biochemistry

JF - Fish Physiology and Biochemistry

SN - 0920-1742

ER -