TY - JOUR
T1 - Characterisation and expression analysis of B-cell activating factor (BAFF) in spiny dogfish (Squalus acanthias)
T2 - cartilaginous fish BAFF has a unique extra exon that may impact receptor binding
AU - Li, Ronggai
AU - Dooley, Helen
AU - Wang, Tiehui
AU - Secombes, Christopher J
AU - Bird, Steve
N1 - Copyright © 2011 Elsevier Ltd. All rights reserved.
PY - 2012/4
Y1 - 2012/4
N2 - B-cell activating factor (BAFF), also known as tumour necrosis factor (TNF) ligand superfamily member 13B, is an important immune regulator with critical roles in B-cell survival, proliferation, differentiation and immunoglobulin secretion. A BAFF gene has been cloned from spiny dogfish (Squalus acanthias) and its expression studied. The dogfish BAFF encodes for an anchored type-II transmembrane protein of 288 aa with a putative furin protease cleavage site and TNF family signature as seen in BAFFs from other species. The identity of dogfish BAFF has also been confirmed by conserved cysteine residues, and phylogenetic tree analysis. The dogfish BAFF gene has an extra exon not seen in teleost fish, birds and mammals that encodes for 29 aa and may impact on receptor binding. The dogfish BAFF is highly expressed in immune tissues, such as spleen, and is up-regulated by PWM in peripheral blood leucocytes, suggesting a potentially important role in the immune system.
AB - B-cell activating factor (BAFF), also known as tumour necrosis factor (TNF) ligand superfamily member 13B, is an important immune regulator with critical roles in B-cell survival, proliferation, differentiation and immunoglobulin secretion. A BAFF gene has been cloned from spiny dogfish (Squalus acanthias) and its expression studied. The dogfish BAFF encodes for an anchored type-II transmembrane protein of 288 aa with a putative furin protease cleavage site and TNF family signature as seen in BAFFs from other species. The identity of dogfish BAFF has also been confirmed by conserved cysteine residues, and phylogenetic tree analysis. The dogfish BAFF gene has an extra exon not seen in teleost fish, birds and mammals that encodes for 29 aa and may impact on receptor binding. The dogfish BAFF is highly expressed in immune tissues, such as spleen, and is up-regulated by PWM in peripheral blood leucocytes, suggesting a potentially important role in the immune system.
KW - spiny dogfish
KW - BAFF
KW - cDNA cloning
KW - gene organisation
KW - tissue distribution
KW - modulation of expression
U2 - 10.1016/j.dci.2011.11.010
DO - 10.1016/j.dci.2011.11.010
M3 - Article
C2 - 22155638
SN - 1879-0089
VL - 36
SP - 707
EP - 717
JO - Developmental and Comparative Immunology
JF - Developmental and Comparative Immunology
IS - 4
ER -