Characterisation and expression analysis of interleukin 2 (IL-2) and IL-21 homologues in the Japanese pufferfish, Fugu rubripes, following their discovery by synteny

Steven Bird, Jun Zou, T Kono, M Sakai, J M Dijkstra, Christopher John Secombes

Research output: Contribution to journalLiterature review

80 Citations (Scopus)

Abstract

This investigation provides the first conclusive evidence for the existence of the interleukin 2 (IL-2) and IL-21 genes in bony fish. The IL-2 and IL-21 sequences have been determined in Fugu rubripes by exploiting the conservation of synteny that is found between regions of the human and Fugu genomes. The predicted 149-amino acid IL-2 homologue contains the IL-2 family signature, has a predicted secondary structure of three a helixes and has the two cysteines important in disulphide-bond formation. It shows low amino acid identities (24-34%) with other known IL-2 sequences. The predicted 155-amino acid IL-21 homologue has a predicted secondary structure of four a helixes and has the four cysteines important in disulphide-bond formation. It shows low amino acid identities (29-31%) with other known IL-21 sequences. The gene organisation of Fugu IL-2 and IL-21 and the level of synteny between the human and Fugu genomes has been well conserved during evolution, with the order and orientation of the genes matching exactly to human Chromosome 4. Phytohaemagglutinin stimulation of Fugu kidney cells resulted in a large increase in the Fugu IL-2 and IL-21 transcripts. In vivo stimulation of Fugu with LPS and poly LC showed IL-21 expression to be localised within mucosal tissues. The discovery of IL-2 and IL-21 in fish will now allow more detailed investigations into T-helper cell responses.

Original languageEnglish
Pages (from-to)909-923
Number of pages15
JournalImmunogenetics
Volume56
Issue number12
DOIs
Publication statusPublished - Mar 2005

Keywords

  • Fugu rubripes
  • cytokine evolution
  • interleukin-2
  • interleukin-21
  • cloning
  • inflammation
  • gene organisation
  • comparative immunology
  • cell growth-factor
  • trout oncorhynchus-mykiss
  • receptor-gamma chain
  • amino-acid-sequence
  • mouse interleukin-2
  • cyprinus-carpio
  • rainbow-trout
  • immune-system
  • alpha-chain
  • beta-chain

Cite this

Characterisation and expression analysis of interleukin 2 (IL-2) and IL-21 homologues in the Japanese pufferfish, Fugu rubripes, following their discovery by synteny. / Bird, Steven; Zou, Jun; Kono, T ; Sakai, M ; Dijkstra, J M ; Secombes, Christopher John.

In: Immunogenetics, Vol. 56, No. 12, 03.2005, p. 909-923.

Research output: Contribution to journalLiterature review

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abstract = "This investigation provides the first conclusive evidence for the existence of the interleukin 2 (IL-2) and IL-21 genes in bony fish. The IL-2 and IL-21 sequences have been determined in Fugu rubripes by exploiting the conservation of synteny that is found between regions of the human and Fugu genomes. The predicted 149-amino acid IL-2 homologue contains the IL-2 family signature, has a predicted secondary structure of three a helixes and has the two cysteines important in disulphide-bond formation. It shows low amino acid identities (24-34{\%}) with other known IL-2 sequences. The predicted 155-amino acid IL-21 homologue has a predicted secondary structure of four a helixes and has the four cysteines important in disulphide-bond formation. It shows low amino acid identities (29-31{\%}) with other known IL-21 sequences. The gene organisation of Fugu IL-2 and IL-21 and the level of synteny between the human and Fugu genomes has been well conserved during evolution, with the order and orientation of the genes matching exactly to human Chromosome 4. Phytohaemagglutinin stimulation of Fugu kidney cells resulted in a large increase in the Fugu IL-2 and IL-21 transcripts. In vivo stimulation of Fugu with LPS and poly LC showed IL-21 expression to be localised within mucosal tissues. The discovery of IL-2 and IL-21 in fish will now allow more detailed investigations into T-helper cell responses.",
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T1 - Characterisation and expression analysis of interleukin 2 (IL-2) and IL-21 homologues in the Japanese pufferfish, Fugu rubripes, following their discovery by synteny

AU - Bird, Steven

AU - Zou, Jun

AU - Kono, T

AU - Sakai, M

AU - Dijkstra, J M

AU - Secombes, Christopher John

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N2 - This investigation provides the first conclusive evidence for the existence of the interleukin 2 (IL-2) and IL-21 genes in bony fish. The IL-2 and IL-21 sequences have been determined in Fugu rubripes by exploiting the conservation of synteny that is found between regions of the human and Fugu genomes. The predicted 149-amino acid IL-2 homologue contains the IL-2 family signature, has a predicted secondary structure of three a helixes and has the two cysteines important in disulphide-bond formation. It shows low amino acid identities (24-34%) with other known IL-2 sequences. The predicted 155-amino acid IL-21 homologue has a predicted secondary structure of four a helixes and has the four cysteines important in disulphide-bond formation. It shows low amino acid identities (29-31%) with other known IL-21 sequences. The gene organisation of Fugu IL-2 and IL-21 and the level of synteny between the human and Fugu genomes has been well conserved during evolution, with the order and orientation of the genes matching exactly to human Chromosome 4. Phytohaemagglutinin stimulation of Fugu kidney cells resulted in a large increase in the Fugu IL-2 and IL-21 transcripts. In vivo stimulation of Fugu with LPS and poly LC showed IL-21 expression to be localised within mucosal tissues. The discovery of IL-2 and IL-21 in fish will now allow more detailed investigations into T-helper cell responses.

AB - This investigation provides the first conclusive evidence for the existence of the interleukin 2 (IL-2) and IL-21 genes in bony fish. The IL-2 and IL-21 sequences have been determined in Fugu rubripes by exploiting the conservation of synteny that is found between regions of the human and Fugu genomes. The predicted 149-amino acid IL-2 homologue contains the IL-2 family signature, has a predicted secondary structure of three a helixes and has the two cysteines important in disulphide-bond formation. It shows low amino acid identities (24-34%) with other known IL-2 sequences. The predicted 155-amino acid IL-21 homologue has a predicted secondary structure of four a helixes and has the four cysteines important in disulphide-bond formation. It shows low amino acid identities (29-31%) with other known IL-21 sequences. The gene organisation of Fugu IL-2 and IL-21 and the level of synteny between the human and Fugu genomes has been well conserved during evolution, with the order and orientation of the genes matching exactly to human Chromosome 4. Phytohaemagglutinin stimulation of Fugu kidney cells resulted in a large increase in the Fugu IL-2 and IL-21 transcripts. In vivo stimulation of Fugu with LPS and poly LC showed IL-21 expression to be localised within mucosal tissues. The discovery of IL-2 and IL-21 in fish will now allow more detailed investigations into T-helper cell responses.

KW - Fugu rubripes

KW - cytokine evolution

KW - interleukin-2

KW - interleukin-21

KW - cloning

KW - inflammation

KW - gene organisation

KW - comparative immunology

KW - cell growth-factor

KW - trout oncorhynchus-mykiss

KW - receptor-gamma chain

KW - amino-acid-sequence

KW - mouse interleukin-2

KW - cyprinus-carpio

KW - rainbow-trout

KW - immune-system

KW - alpha-chain

KW - beta-chain

U2 - 10.1007/s00251-004-0741-7

DO - 10.1007/s00251-004-0741-7

M3 - Literature review

VL - 56

SP - 909

EP - 923

JO - Immunogenetics

JF - Immunogenetics

SN - 0093-7711

IS - 12

ER -