Characterisation and pathogenicity of Fusarium taxa isolated from ragwort (Jacobaea vulgaris) roots

K. A. Pearson, A. F. S. Taylor, R. M. E. Fuchs, S. Woodward*

*Corresponding author for this work

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We characterised the fungi isolated from necrotic lesions observed on roots of the noxious weedJacobaea vulgaris and assessed their host range. ITS rDNA sequence data identified twenty-one isolates of Fusarium and TEF-1 alpha sequencing separated these isolates into four clades namely Fusarium avenaceum/Fusarium acuminatum, Fusarium redolens, Fusarium culmorum/Fusarium cerealis and Fusarium solani Representatives of each Glade were tested for virulence against ragwort seedlings. All isolates in the F. avenaceum/E acuminatum Glade caused visible disease symptoms. Host range of three isolates was tested using six pasture grass species along with Trifolium pratense and Trifolium repens. No disease symptoms were detected on the grasses. All three isolates, however, caused disease symptoms on T. repens, and two isolates also attacked T. pratense. The results demonstrate that several species of Fusarium can colonise ragwort roots but only isolates from one Glade cause significant disease symptoms on ragwort seedlings. (C) 2016 Elsevier Ltd and The British Mycological Society. All rights reserved.

Original languageEnglish
Pages (from-to)186-192
Number of pages7
JournalFungal Ecology
Volume20
Early online date12 Feb 2016
DOIs
Publication statusPublished - Apr 2016

Keywords

  • Jacobaea vulgaris
  • Senecio jacobaea
  • Root pathogens
  • Fusarium
  • Ragwort
  • biological-control agents
  • solani species complex
  • pyrrolizidine alkaloids
  • host-range
  • phylogeny
  • fungi
  • disease
  • weeds
  • rot

Cite this

Characterisation and pathogenicity of Fusarium taxa isolated from ragwort (Jacobaea vulgaris) roots. / Pearson, K. A.; Taylor, A. F. S.; Fuchs, R. M. E.; Woodward, S.

In: Fungal Ecology, Vol. 20, 04.2016, p. 186-192.

Research output: Contribution to journalArticle

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abstract = "We characterised the fungi isolated from necrotic lesions observed on roots of the noxious weedJacobaea vulgaris and assessed their host range. ITS rDNA sequence data identified twenty-one isolates of Fusarium and TEF-1 alpha sequencing separated these isolates into four clades namely Fusarium avenaceum/Fusarium acuminatum, Fusarium redolens, Fusarium culmorum/Fusarium cerealis and Fusarium solani Representatives of each Glade were tested for virulence against ragwort seedlings. All isolates in the F. avenaceum/E acuminatum Glade caused visible disease symptoms. Host range of three isolates was tested using six pasture grass species along with Trifolium pratense and Trifolium repens. No disease symptoms were detected on the grasses. All three isolates, however, caused disease symptoms on T. repens, and two isolates also attacked T. pratense. The results demonstrate that several species of Fusarium can colonise ragwort roots but only isolates from one Glade cause significant disease symptoms on ragwort seedlings. (C) 2016 Elsevier Ltd and The British Mycological Society. All rights reserved.",
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N2 - We characterised the fungi isolated from necrotic lesions observed on roots of the noxious weedJacobaea vulgaris and assessed their host range. ITS rDNA sequence data identified twenty-one isolates of Fusarium and TEF-1 alpha sequencing separated these isolates into four clades namely Fusarium avenaceum/Fusarium acuminatum, Fusarium redolens, Fusarium culmorum/Fusarium cerealis and Fusarium solani Representatives of each Glade were tested for virulence against ragwort seedlings. All isolates in the F. avenaceum/E acuminatum Glade caused visible disease symptoms. Host range of three isolates was tested using six pasture grass species along with Trifolium pratense and Trifolium repens. No disease symptoms were detected on the grasses. All three isolates, however, caused disease symptoms on T. repens, and two isolates also attacked T. pratense. The results demonstrate that several species of Fusarium can colonise ragwort roots but only isolates from one Glade cause significant disease symptoms on ragwort seedlings. (C) 2016 Elsevier Ltd and The British Mycological Society. All rights reserved.

AB - We characterised the fungi isolated from necrotic lesions observed on roots of the noxious weedJacobaea vulgaris and assessed their host range. ITS rDNA sequence data identified twenty-one isolates of Fusarium and TEF-1 alpha sequencing separated these isolates into four clades namely Fusarium avenaceum/Fusarium acuminatum, Fusarium redolens, Fusarium culmorum/Fusarium cerealis and Fusarium solani Representatives of each Glade were tested for virulence against ragwort seedlings. All isolates in the F. avenaceum/E acuminatum Glade caused visible disease symptoms. Host range of three isolates was tested using six pasture grass species along with Trifolium pratense and Trifolium repens. No disease symptoms were detected on the grasses. All three isolates, however, caused disease symptoms on T. repens, and two isolates also attacked T. pratense. The results demonstrate that several species of Fusarium can colonise ragwort roots but only isolates from one Glade cause significant disease symptoms on ragwort seedlings. (C) 2016 Elsevier Ltd and The British Mycological Society. All rights reserved.

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