Characterisation and PCR multiplexing of novel highly variable tetranucleotide Atlantic salmon (Salmo salar L.) microsatellites

S. Paterson, Stuart Brannon Piertney, D. J. Knox, J. Gilbey, Eric Verspoor

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Abstract

Seven novel and highly variable tetranucleotide microsatellite markers, and conditions for multiplexing and simultaneous genotyping six of these in a single run, are described for Atlantic salmon. These provide a highly informative and cost-effective set of molecular markers for genetic studies on cultured and wild populations of the species. The primers sets showed cross-species amplification of appropriately sized amplified products in a number of other salmonid species and suggests the primer sets may have wider application.

Original languageEnglish
Pages (from-to)160-162
Number of pages2
JournalMolecular Ecology Notes
Volume4
DOIs
Publication statusPublished - 2004

Keywords

  • Atlantic salmon
  • microsatellite
  • multiplex
  • Salmo salar
  • MARKERS

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