Characterisation of a pea hsp70 gene which is both developmentally and stress-regulated

O P Dhankher, Janice Drew, J A Gatehouse

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A pea pod cDNA library was screened for sequences specific to lignifying tissue. A cDNA clone (pLP19) encoding the C-terminal region of a hsp70 heat shock protein hybridised only to pod mRNA from pea lines where pod lignification occurred. Expression of pLP19 was induced by heat shock in leaves, stems and roots of pea and chickpea plants. Four different poly(A) addition sites were observed in cDNAs derived from the same gene as pLP19. This gene was fully sequenced; unlike most hsp70 genes, it contains no introns. The 5'-flanking sequence contains heat shock elements and other potential regulatory sequences.
Original languageEnglish
Pages (from-to)345-52
Number of pages8
JournalPlant Molecular Biology
Volume34
Issue number2
Publication statusPublished - 1 May 1997

Fingerprint

Peas
pods
peas
heat stress
Shock
Complementary DNA
Hot Temperature
Genes
Cicer
regulatory sequences
Poly A
genes
lignification
5' Flanking Region
Heat-Shock Proteins
Gene Library
heat shock proteins
cDNA libraries
Introns
introns

Keywords

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary
  • DNA, Plant
  • Gene Expression Regulation, Developmental
  • Genes, Plant
  • HSP70 Heat-Shock Proteins
  • Molecular Sequence Data
  • Peas
  • Plant Proteins
  • RNA, Messenger
  • Sequence Analysis, DNA

Cite this

Characterisation of a pea hsp70 gene which is both developmentally and stress-regulated. / Dhankher, O P; Drew, Janice; Gatehouse, J A.

In: Plant Molecular Biology, Vol. 34, No. 2, 01.05.1997, p. 345-52.

Research output: Contribution to journalArticle

@article{6053ccfde8c6432080b72747a9d4625a,
title = "Characterisation of a pea hsp70 gene which is both developmentally and stress-regulated",
abstract = "A pea pod cDNA library was screened for sequences specific to lignifying tissue. A cDNA clone (pLP19) encoding the C-terminal region of a hsp70 heat shock protein hybridised only to pod mRNA from pea lines where pod lignification occurred. Expression of pLP19 was induced by heat shock in leaves, stems and roots of pea and chickpea plants. Four different poly(A) addition sites were observed in cDNAs derived from the same gene as pLP19. This gene was fully sequenced; unlike most hsp70 genes, it contains no introns. The 5'-flanking sequence contains heat shock elements and other potential regulatory sequences.",
keywords = "Amino Acid Sequence, Base Sequence, Cloning, Molecular, DNA, Complementary, DNA, Plant, Gene Expression Regulation, Developmental, Genes, Plant, HSP70 Heat-Shock Proteins, Molecular Sequence Data, Peas, Plant Proteins, RNA, Messenger, Sequence Analysis, DNA",
author = "Dhankher, {O P} and Janice Drew and Gatehouse, {J A}",
year = "1997",
month = "5",
day = "1",
language = "English",
volume = "34",
pages = "345--52",
journal = "Plant Molecular Biology",
issn = "0167-4412",
publisher = "Springer Netherlands",
number = "2",

}

TY - JOUR

T1 - Characterisation of a pea hsp70 gene which is both developmentally and stress-regulated

AU - Dhankher, O P

AU - Drew, Janice

AU - Gatehouse, J A

PY - 1997/5/1

Y1 - 1997/5/1

N2 - A pea pod cDNA library was screened for sequences specific to lignifying tissue. A cDNA clone (pLP19) encoding the C-terminal region of a hsp70 heat shock protein hybridised only to pod mRNA from pea lines where pod lignification occurred. Expression of pLP19 was induced by heat shock in leaves, stems and roots of pea and chickpea plants. Four different poly(A) addition sites were observed in cDNAs derived from the same gene as pLP19. This gene was fully sequenced; unlike most hsp70 genes, it contains no introns. The 5'-flanking sequence contains heat shock elements and other potential regulatory sequences.

AB - A pea pod cDNA library was screened for sequences specific to lignifying tissue. A cDNA clone (pLP19) encoding the C-terminal region of a hsp70 heat shock protein hybridised only to pod mRNA from pea lines where pod lignification occurred. Expression of pLP19 was induced by heat shock in leaves, stems and roots of pea and chickpea plants. Four different poly(A) addition sites were observed in cDNAs derived from the same gene as pLP19. This gene was fully sequenced; unlike most hsp70 genes, it contains no introns. The 5'-flanking sequence contains heat shock elements and other potential regulatory sequences.

KW - Amino Acid Sequence

KW - Base Sequence

KW - Cloning, Molecular

KW - DNA, Complementary

KW - DNA, Plant

KW - Gene Expression Regulation, Developmental

KW - Genes, Plant

KW - HSP70 Heat-Shock Proteins

KW - Molecular Sequence Data

KW - Peas

KW - Plant Proteins

KW - RNA, Messenger

KW - Sequence Analysis, DNA

M3 - Article

C2 - 9207851

VL - 34

SP - 345

EP - 352

JO - Plant Molecular Biology

JF - Plant Molecular Biology

SN - 0167-4412

IS - 2

ER -