Characterisation of a pea hsp70 gene which is both developmentally and stress-regulated

O P Dhankher, Janice Drew, J A Gatehouse

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A pea pod cDNA library was screened for sequences specific to lignifying tissue. A cDNA clone (pLP19) encoding the C-terminal region of a hsp70 heat shock protein hybridised only to pod mRNA from pea lines where pod lignification occurred. Expression of pLP19 was induced by heat shock in leaves, stems and roots of pea and chickpea plants. Four different poly(A) addition sites were observed in cDNAs derived from the same gene as pLP19. This gene was fully sequenced; unlike most hsp70 genes, it contains no introns. The 5'-flanking sequence contains heat shock elements and other potential regulatory sequences.
Original languageEnglish
Pages (from-to)345-52
Number of pages8
JournalPlant Molecular Biology
Volume34
Issue number2
Publication statusPublished - 1 May 1997

Keywords

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary
  • DNA, Plant
  • Gene Expression Regulation, Developmental
  • Genes, Plant
  • HSP70 Heat-Shock Proteins
  • Molecular Sequence Data
  • Peas
  • Plant Proteins
  • RNA, Messenger
  • Sequence Analysis, DNA

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