Characterisation of a pea hsp70 gene which is both developmentally and stress-regulated

O P Dhankher; Janice Drew; J A Gatehouse

Characterisation of a pea hsp70 gene which is both developmentally and stress-regulated

O P Dhankher, Janice Drew, J A Gatehouse

The Rowett Institute of Nutrition and Health

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

A pea pod cDNA library was screened for sequences specific to lignifying tissue. A cDNA clone (pLP19) encoding the C-terminal region of a hsp70 heat shock protein hybridised only to pod mRNA from pea lines where pod lignification occurred. Expression of pLP19 was induced by heat shock in leaves, stems and roots of pea and chickpea plants. Four different poly(A) addition sites were observed in cDNAs derived from the same gene as pLP19. This gene was fully sequenced; unlike most hsp70 genes, it contains no introns. The 5'-flanking sequence contains heat shock elements and other potential regulatory sequences.

Original language	English
Pages (from-to)	345-52
Number of pages	8
Journal	Plant Molecular Biology
Volume	34
Issue number	2
Publication status	Published - 1 May 1997

Keywords

Amino Acid Sequence
Base Sequence
Cloning, Molecular
DNA, Complementary
DNA, Plant
Gene Expression Regulation, Developmental
Genes, Plant
HSP70 Heat-Shock Proteins
Molecular Sequence Data
Peas
Plant Proteins
RNA, Messenger
Sequence Analysis, DNA

Cite this

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title = "Characterisation of a pea hsp70 gene which is both developmentally and stress-regulated",

abstract = "A pea pod cDNA library was screened for sequences specific to lignifying tissue. A cDNA clone (pLP19) encoding the C-terminal region of a hsp70 heat shock protein hybridised only to pod mRNA from pea lines where pod lignification occurred. Expression of pLP19 was induced by heat shock in leaves, stems and roots of pea and chickpea plants. Four different poly(A) addition sites were observed in cDNAs derived from the same gene as pLP19. This gene was fully sequenced; unlike most hsp70 genes, it contains no introns. The 5'-flanking sequence contains heat shock elements and other potential regulatory sequences.",

keywords = "Amino Acid Sequence, Base Sequence, Cloning, Molecular, DNA, Complementary, DNA, Plant, Gene Expression Regulation, Developmental, Genes, Plant, HSP70 Heat-Shock Proteins, Molecular Sequence Data, Peas, Plant Proteins, RNA, Messenger, Sequence Analysis, DNA",

author = "Dhankher, {O P} and Janice Drew and Gatehouse, {J A}",

year = "1997",

month = may,

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language = "English",

volume = "34",

pages = "345--52",

journal = "Plant Molecular Biology",

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TY - JOUR

T1 - Characterisation of a pea hsp70 gene which is both developmentally and stress-regulated

AU - Dhankher, O P

AU - Drew, Janice

AU - Gatehouse, J A

PY - 1997/5/1

Y1 - 1997/5/1

N2 - A pea pod cDNA library was screened for sequences specific to lignifying tissue. A cDNA clone (pLP19) encoding the C-terminal region of a hsp70 heat shock protein hybridised only to pod mRNA from pea lines where pod lignification occurred. Expression of pLP19 was induced by heat shock in leaves, stems and roots of pea and chickpea plants. Four different poly(A) addition sites were observed in cDNAs derived from the same gene as pLP19. This gene was fully sequenced; unlike most hsp70 genes, it contains no introns. The 5'-flanking sequence contains heat shock elements and other potential regulatory sequences.

AB - A pea pod cDNA library was screened for sequences specific to lignifying tissue. A cDNA clone (pLP19) encoding the C-terminal region of a hsp70 heat shock protein hybridised only to pod mRNA from pea lines where pod lignification occurred. Expression of pLP19 was induced by heat shock in leaves, stems and roots of pea and chickpea plants. Four different poly(A) addition sites were observed in cDNAs derived from the same gene as pLP19. This gene was fully sequenced; unlike most hsp70 genes, it contains no introns. The 5'-flanking sequence contains heat shock elements and other potential regulatory sequences.

KW - Amino Acid Sequence

KW - Base Sequence

KW - Cloning, Molecular

KW - DNA, Complementary

KW - DNA, Plant

KW - Gene Expression Regulation, Developmental

KW - Genes, Plant

KW - HSP70 Heat-Shock Proteins

KW - Molecular Sequence Data

KW - Peas

KW - Plant Proteins

KW - RNA, Messenger

KW - Sequence Analysis, DNA

M3 - Article

C2 - 9207851

SN - 0167-4412

VL - 34

SP - 345

EP - 352

JO - Plant Molecular Biology

JF - Plant Molecular Biology

IS - 2

ER -

Characterisation of a pea hsp70 gene which is both developmentally and stress-regulated

Abstract

Keywords

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