Characterisation of endobacterial communities in ectomycorrhizas by DNA- and RNA-based molecular methods

Hironari Izumi, Edward R. B. Moore, Ken Killham, Ian J. Alexander, Ian C. Anderson

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The diversity of endobacteria associated with ectomycorrhizas of Suillus variegatus and Tomentellopsis submollis, in two Corsican pine (Pinus nigra) stands was analysed by cultivation-dependent and cultivation-independent molecular methods. Denaturing gradient gel electrophoresis (DGGE) analysis revealed the cultivable endobacterial communities associated with S. variegatus were similar within the same stand. The most abundant cultivable bacterial species belonged to the genera Pseudomonas and Burkholderia. Cultivation-independent molecular analysis indicated that the structure of the endobacterial communities in ectomycorrhizas was consistent across all samples regardless of ECM fungal species or the pine stand from which the samples were collected. However, comparison between rDNA- and rRNA-derived DGGE gels showed that metabolically active endobacterial species were not always detected in rDNA-based profiles. Clone libraries constructed from rRNA molecules indicated that Pseudomonas and Burkholderia spp. were metabolically active bacteria. As some of the most abundant cultivable bacteria, including Bacillus/Paenibacillus spp., were not detected in cultivation-independent DGGE profiles, a combination of cultivation-dependent and -independent approaches provided a more complete assessment of the diversity of endobacteria associated with ectomycorrhizas. (c) 2006 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)891-899
Number of pages9
JournalSoil Biology and Biochemistry
Volume39
Issue number4
Early online date6 Dec 2006
DOIs
Publication statusPublished - Apr 2007

Keywords

  • endobacteria
  • 16S rRNA
  • metabolically active bacteria
  • ectomycorrhiza
  • gradient gel-electrophoresis
  • 16S ribosomal-RNA
  • tuber-borchii vittad
  • in-situ detection
  • pinus-sylvestris
  • bacterial community
  • helper bacteria
  • populations
  • endophyte
  • growth

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