Characterisation of innate fungal recognition in the lung

Inês Faro-Trindade, Janet A Willment, Ann M Kerrigan, Pierre Redelinghuys, Sabelo Hadebe, Delyth M Reid, Naren Srinivasan, Helen Wainwright, Dirk M Lang, Chad Steele, Gordon D Brown

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Abstract

The innate recognition of fungi by leukocytes is mediated by pattern recognition receptors (PRR), such as Dectin-1, and is thought to occur at the cell surface triggering intracellular signalling cascades which lead to the induction of protective host responses. In the lung, this recognition is aided by surfactant which also serves to maintain the balance between inflammation and pulmonary function, although the underlying mechanisms are unknown. Here we have explored pulmonary innate recognition of a variety of fungal particles, including zymosan, Candida albicans and Aspergillus fumigatus, and demonstrate that opsonisation with surfactant components can limit inflammation by reducing host-cell fungal interactions. However, we found that this opsonisation does not contribute directly to innate fungal recognition and that this process is mediated through non-opsonic PRRs, including Dectin-1. Moreover, we found that pulmonary inflammatory responses to resting Aspergillus conidia were initiated by these PRRs in acidified phagolysosomes, following the uptake of fungal particles by leukocytes. Our data therefore provides crucial new insights into the mechanisms by which surfactant can maintain pulmonary function in the face of microbial challenge, and defines the phagolysosome as a novel intracellular compartment involved in the innate sensing of extracellular pathogens in the lung.
Original languageEnglish
Article numbere35675
JournalPloS ONE
Volume7
Issue number4
DOIs
Publication statusPublished - 20 Apr 2012

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Surface-Active Agents
Aspergillus
lungs
surfactants
phagosomes
Lung
inflammation
lung function
Phagosomes
Pattern Recognition Receptors
leukocytes
Zymosan
Candida
Pathogens
Fungi
Leukocytes
zymosan
Aspergillus fumigatus
Candida albicans
Fungal Spores

Cite this

Faro-Trindade, I., Willment, J. A., Kerrigan, A. M., Redelinghuys, P., Hadebe, S., Reid, D. M., ... Brown, G. D. (2012). Characterisation of innate fungal recognition in the lung. PloS ONE, 7(4), [e35675]. https://doi.org/10.1371/journal.pone.0035675

Characterisation of innate fungal recognition in the lung. / Faro-Trindade, Inês; Willment, Janet A; Kerrigan, Ann M; Redelinghuys, Pierre; Hadebe, Sabelo; Reid, Delyth M; Srinivasan, Naren; Wainwright, Helen; Lang, Dirk M; Steele, Chad; Brown, Gordon D.

In: PloS ONE, Vol. 7, No. 4, e35675, 20.04.2012.

Research output: Contribution to journalArticle

Faro-Trindade, I, Willment, JA, Kerrigan, AM, Redelinghuys, P, Hadebe, S, Reid, DM, Srinivasan, N, Wainwright, H, Lang, DM, Steele, C & Brown, GD 2012, 'Characterisation of innate fungal recognition in the lung' PloS ONE, vol. 7, no. 4, e35675. https://doi.org/10.1371/journal.pone.0035675
Faro-Trindade I, Willment JA, Kerrigan AM, Redelinghuys P, Hadebe S, Reid DM et al. Characterisation of innate fungal recognition in the lung. PloS ONE. 2012 Apr 20;7(4). e35675. https://doi.org/10.1371/journal.pone.0035675
Faro-Trindade, Inês ; Willment, Janet A ; Kerrigan, Ann M ; Redelinghuys, Pierre ; Hadebe, Sabelo ; Reid, Delyth M ; Srinivasan, Naren ; Wainwright, Helen ; Lang, Dirk M ; Steele, Chad ; Brown, Gordon D. / Characterisation of innate fungal recognition in the lung. In: PloS ONE. 2012 ; Vol. 7, No. 4.
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