Characterisation of ribosomal satellite in total nuclear DNA from Physarum polycephalum

Norman Hardman*, Peter L. Jack, Alistair J P Brown, Alan McLachlan

*Corresponding author for this work

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The distinctive properties of satellite DNA molecules containing the genes for ribosomal RNA in Physarum polycephalum permits their identification in total, unfractionated nuclear DNA in the foldback form, after denaturation and fast annealing. Using the electron microscope the location and properties of three characteristic regions containing tandemly-repeated, inverted sequences have been investigated. At least two additional regions, also containing tandem repeats, are shown to be present and located towards each end of the rDNA molecule, at a site adjacent to the segment coding for the 26 S rRNA. All the regions which contain tandem repeats are composed of sequences which, within experimental error, appear to share a common unit repeat length of about 90 nucleotides.

Original languageEnglish
Pages (from-to)365-376
Number of pages12
JournalBBA Section Nucleic Acids And Protein Synthesis
Volume562
Issue number3
DOIs
Publication statusPublished - 24 May 1979

Keywords

  • (Physarum)
  • rDNA
  • rRNA
  • Satellite DNA

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