Characterisation of ZBTB46 and DC-SCRIPT/ZNF366 in rainbow trout, transcription factors potentially involved in dendritic cell maturation and activation in fish

Jun Wang, Tiehui Wang, Ottavia Benedicenti, Catherine Collins, Kaiyu Wang, Christopher J. Secombes, Jun Zou

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

ZBTB46 and DC-SCRIPT/ZNF366 are two zinc finger transcription factors that play important roles in regulating differentiation of dendritic cells in mammals. In this study, the ZBTB46 and DC-SCRIPT/ZNF366 homologues were identified in rainbow trout Oncorhynchus mykiss and their expression analysed in vivo and in vitro. As transcription factors, they are well conserved in sequence, genomic organisation and gene synteny. Their expression was differentially modulated by bacterial and viral PAMPs in the monocyte/macrophage like cell line RTS-11, in primary head kidney (HK) macrophages, and in HK macrophages cultured with IL-4/13A. In the RTS-11 cells and primary HK macrophages, all the ZBTB46 and DC-SCRIPT/ZNF366 homologues were down-regulated by interferon gamma (type II IFN) but unaffected by IFN2 (type I IFN), administered as recombinant proteins to cell cultures. In fish gills, infection with amoebae (Paramoebae perurans) resulted in reduction of ZBTB46 and DC-SCRIPT/ZNF366 expression in Atlantic salmon Salmo salar, whilst infection with Yersinia ruckeri induced gene expression in rainbow trout.

Original languageEnglish
Pages (from-to)2-14
Number of pages13
JournalDevelopmental and Comparative Immunology
Volume80
Early online date23 Nov 2016
DOIs
Publication statusPublished - Mar 2018

Fingerprint

Oncorhynchus mykiss
Head Kidney
Dendritic Cells
Fishes
Transcription Factors
Macrophages
Salmo salar
Interferon-gamma
Yersinia ruckeri
Synteny
Amoeba
Conserved Sequence
Zinc Fingers
Infection
Recombinant Proteins
Interleukin-4
Monocytes
Mammals
Cell Culture Techniques
Gene Expression

Keywords

  • ZBTB46
  • DC-SCRIPT/ZNF366
  • Dendritic cell
  • Transcription factor
  • Gene expression
  • Interferon

Cite this

Characterisation of ZBTB46 and DC-SCRIPT/ZNF366 in rainbow trout, transcription factors potentially involved in dendritic cell maturation and activation in fish. / Wang, Jun; Wang, Tiehui; Benedicenti, Ottavia; Collins, Catherine; Wang, Kaiyu; Secombes, Christopher J.; Zou, Jun.

In: Developmental and Comparative Immunology, Vol. 80, 03.2018, p. 2-14.

Research output: Contribution to journalArticle

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abstract = "ZBTB46 and DC-SCRIPT/ZNF366 are two zinc finger transcription factors that play important roles in regulating differentiation of dendritic cells in mammals. In this study, the ZBTB46 and DC-SCRIPT/ZNF366 homologues were identified in rainbow trout Oncorhynchus mykiss and their expression analysed in vivo and in vitro. As transcription factors, they are well conserved in sequence, genomic organisation and gene synteny. Their expression was differentially modulated by bacterial and viral PAMPs in the monocyte/macrophage like cell line RTS-11, in primary head kidney (HK) macrophages, and in HK macrophages cultured with IL-4/13A. In the RTS-11 cells and primary HK macrophages, all the ZBTB46 and DC-SCRIPT/ZNF366 homologues were down-regulated by interferon gamma (type II IFN) but unaffected by IFN2 (type I IFN), administered as recombinant proteins to cell cultures. In fish gills, infection with amoebae (Paramoebae perurans) resulted in reduction of ZBTB46 and DC-SCRIPT/ZNF366 expression in Atlantic salmon Salmo salar, whilst infection with Yersinia ruckeri induced gene expression in rainbow trout.",
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