Characterization and expression analysis of chemokine-like receptor 3 gene in rainbow trout Oncorhynchus mykiss

Zhitao Qi*, Qihuan Zhang, Jason W. Holland, Qian Gao, Carolina Tafalla, Xiuchun Wang, Tiehui Wang

*Corresponding author for this work

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Chemokine-like receptors (CMKLRs) are multi-functional receptors with roles in regulating leukocyte and inflammation. Currently, two members of the CMKLRs, CMKLR1 and CMKLR2, have been found in salmonids, indicating that the CMKLRs had expanded from an ancestor gene in teleost fish. In the present study, the third member of the CMKLRs, defined as CMKLR3, was identified and cloned in rainbow trout. The trout CMKLR3 possessed conserved features of the CMKLR family including seven transmembrane regions, a dynein regulatory complex (DRC) motif, and two cysteine residues, but shared low sequence identities with fish CMKLR1 and CMKLR2 (24‒38 %), which was confirmed by phylogenetic tree analysis. Trout CMKLR3 was highly expressed in body kidney, head kidney, and IgM+ B cells, indicating its functional role in regulating leukocytes. We were able to modulate the expression of trout CMKLR3 in vivo by bacterial and parasitic infections but it remained apathetic to virus infection, and it was also successfully modulated in vitro by peptidoglycan and cytokines (IFN-γ and IL-6). Our results suggest that trout CMKLR3 is regulated in a complex way and has an important regulatory role in inflammatory responses.

Original languageEnglish
Pages (from-to)613-622
Number of pages10
JournalReviews in Fisheries Science
Volume82
Issue number4
Early online date20 Jun 2016
DOIs
Publication statusPublished - 1 Jul 2016

Fingerprint

chemokines
rainbow
Oncorhynchus mykiss
trout
receptors
functional role
gene
fish
teleost
ancestry
virus
genes
phylogenetics
leukocytes
inflammation
kidneys
peptidoglycans
parasitoses
Salmonidae
bacterial infections

Keywords

  • Chemokine-like receptors
  • CMKLR3
  • Expression
  • Oncorhynchus mykiss
  • Rainbow trout

ASJC Scopus subject areas

  • Aquatic Science

Cite this

Characterization and expression analysis of chemokine-like receptor 3 gene in rainbow trout Oncorhynchus mykiss. / Qi, Zhitao; Zhang, Qihuan; Holland, Jason W.; Gao, Qian; Tafalla, Carolina; Wang, Xiuchun; Wang, Tiehui.

In: Reviews in Fisheries Science, Vol. 82, No. 4, 01.07.2016, p. 613-622.

Research output: Contribution to journalArticle

Qi, Zhitao ; Zhang, Qihuan ; Holland, Jason W. ; Gao, Qian ; Tafalla, Carolina ; Wang, Xiuchun ; Wang, Tiehui. / Characterization and expression analysis of chemokine-like receptor 3 gene in rainbow trout Oncorhynchus mykiss. In: Reviews in Fisheries Science. 2016 ; Vol. 82, No. 4. pp. 613-622.
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abstract = "Chemokine-like receptors (CMKLRs) are multi-functional receptors with roles in regulating leukocyte and inflammation. Currently, two members of the CMKLRs, CMKLR1 and CMKLR2, have been found in salmonids, indicating that the CMKLRs had expanded from an ancestor gene in teleost fish. In the present study, the third member of the CMKLRs, defined as CMKLR3, was identified and cloned in rainbow trout. The trout CMKLR3 possessed conserved features of the CMKLR family including seven transmembrane regions, a dynein regulatory complex (DRC) motif, and two cysteine residues, but shared low sequence identities with fish CMKLR1 and CMKLR2 (24‒38 {\%}), which was confirmed by phylogenetic tree analysis. Trout CMKLR3 was highly expressed in body kidney, head kidney, and IgM+ B cells, indicating its functional role in regulating leukocytes. We were able to modulate the expression of trout CMKLR3 in vivo by bacterial and parasitic infections but it remained apathetic to virus infection, and it was also successfully modulated in vitro by peptidoglycan and cytokines (IFN-γ and IL-6). Our results suggest that trout CMKLR3 is regulated in a complex way and has an important regulatory role in inflammatory responses.",
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note = "Acknowledgments This work was supported by the Scottish Funding Council for the MASTS (the Marine Alliance for Science and Technology for Scotland) (grant reference HR09011) and partially by grants from the National Natural Science Foundation of China (31302221, 31272666, and 31470130). ZQ was financially supported by the “Qinglan” project of Jiangsu Province and the overseas training plan for young and middle-aged teachers and principals of colleges and universities in Jiangsu Province, China. We thank Professor Mingxian Chang, State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, the Chinese Academy of Sciences, for her kind comments on the phylogenetic tree analysis.",
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N1 - Acknowledgments This work was supported by the Scottish Funding Council for the MASTS (the Marine Alliance for Science and Technology for Scotland) (grant reference HR09011) and partially by grants from the National Natural Science Foundation of China (31302221, 31272666, and 31470130). ZQ was financially supported by the “Qinglan” project of Jiangsu Province and the overseas training plan for young and middle-aged teachers and principals of colleges and universities in Jiangsu Province, China. We thank Professor Mingxian Chang, State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, the Chinese Academy of Sciences, for her kind comments on the phylogenetic tree analysis.

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N2 - Chemokine-like receptors (CMKLRs) are multi-functional receptors with roles in regulating leukocyte and inflammation. Currently, two members of the CMKLRs, CMKLR1 and CMKLR2, have been found in salmonids, indicating that the CMKLRs had expanded from an ancestor gene in teleost fish. In the present study, the third member of the CMKLRs, defined as CMKLR3, was identified and cloned in rainbow trout. The trout CMKLR3 possessed conserved features of the CMKLR family including seven transmembrane regions, a dynein regulatory complex (DRC) motif, and two cysteine residues, but shared low sequence identities with fish CMKLR1 and CMKLR2 (24‒38 %), which was confirmed by phylogenetic tree analysis. Trout CMKLR3 was highly expressed in body kidney, head kidney, and IgM+ B cells, indicating its functional role in regulating leukocytes. We were able to modulate the expression of trout CMKLR3 in vivo by bacterial and parasitic infections but it remained apathetic to virus infection, and it was also successfully modulated in vitro by peptidoglycan and cytokines (IFN-γ and IL-6). Our results suggest that trout CMKLR3 is regulated in a complex way and has an important regulatory role in inflammatory responses.

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