CHARACTERIZATION AND POSTNATAL-DEVELOPMENT OF 5'-DEIODINASE ACTIVITY IN GOAT PERIRENAL FAT

Fergus Nicol, H LEFRANC, John Arthur, P TRAYHURN

Research output: Contribution to journalArticle

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Abstract

The capacity to generate the active thyroid hormone 3,3',5-triiodothyronine (T-3) from thyroxine (T-4) has been assessed in the major adipose tissue depot (perirenal) of newborn goats through measurements of iodothyronine 5'-deiodinase activity. High activity of the enzyme was found in the perirenal adipose tissue. Developmental studies demonstrated that there was a rise, then a fall, in iodothyronine 5'-deiodinase activity (per milligram of protein) over the first week of postnatal life, with a further decline between the second and third weeks. At 3 wk of age, the activity per gram of tissue was only 2% of that of the newborn; however, because of age-related increases in the lipid content of the perirenal adipose tissue, the activity per cell (per mu g of DNA) at 3 wk of age was similar to 10% of the activity in the newborn. Kinetic studies and inhibition characteristics with propylthiouracil, gold thioglucose, and T-4 indicated that the iodothyronine 5'-deiodinase in goat perirenal adipose tissue is of the type I form; there was no evidence for the type II enzyme. Affinity labeling of the iodothyronine 5'-deiodinase with bromoacetyl-rT(3) or bromoacetyl-T-4 was consistent with this view. In contrast to goats, only the type II enzyme is evident in rats and mice. It is concluded that the perirenal (brown) adipose tissue is likely to be a significant source of T-3 for other tissues in newborn goats and that there are major species variations in the form of iodothyronine 5'-deiodinase present in brown fat, with the goat exclusively exhibiting type I.

Original languageEnglish
Number of pages6
JournalAMERICAN JOURNAL OF PHYSIOLOGY
Volume267
Issue number1
Publication statusPublished - Jul 1994

Keywords

  • THERMOGENESIS
  • BROWN ADIPOSE TISSUE
  • THYROXINE
  • TRIIODOTHYRONINE
  • PERIRENAL ADIPOSE TISSUE
  • IODOTHYRONINE 5'-DEIODINASE
  • BROWN ADIPOSE-TISSUE
  • I IODOTHYRONINE DEIODINASE
  • PROTEIN DISULFIDE-ISOMERASE
  • THYROID-HORMONE METABOLISM
  • SELENIUM DEFICIENCY
  • 5'-DEIODINASE ACTIVITY
  • THYROXINE 5'-MONODEIODINATION
  • TRIIODOTHYRONINE PRODUCTION
  • UNCOUPLING PROTEIN
  • 2 PATHWAYS

Cite this

CHARACTERIZATION AND POSTNATAL-DEVELOPMENT OF 5'-DEIODINASE ACTIVITY IN GOAT PERIRENAL FAT. / Nicol, Fergus; LEFRANC, H ; Arthur, John; TRAYHURN, P .

In: AMERICAN JOURNAL OF PHYSIOLOGY, Vol. 267, No. 1, 07.1994.

Research output: Contribution to journalArticle

Nicol, Fergus ; LEFRANC, H ; Arthur, John ; TRAYHURN, P . / CHARACTERIZATION AND POSTNATAL-DEVELOPMENT OF 5'-DEIODINASE ACTIVITY IN GOAT PERIRENAL FAT. In: AMERICAN JOURNAL OF PHYSIOLOGY. 1994 ; Vol. 267, No. 1.
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AU - Nicol, Fergus

AU - LEFRANC, H

AU - Arthur, John

AU - TRAYHURN, P

PY - 1994/7

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N2 - The capacity to generate the active thyroid hormone 3,3',5-triiodothyronine (T-3) from thyroxine (T-4) has been assessed in the major adipose tissue depot (perirenal) of newborn goats through measurements of iodothyronine 5'-deiodinase activity. High activity of the enzyme was found in the perirenal adipose tissue. Developmental studies demonstrated that there was a rise, then a fall, in iodothyronine 5'-deiodinase activity (per milligram of protein) over the first week of postnatal life, with a further decline between the second and third weeks. At 3 wk of age, the activity per gram of tissue was only 2% of that of the newborn; however, because of age-related increases in the lipid content of the perirenal adipose tissue, the activity per cell (per mu g of DNA) at 3 wk of age was similar to 10% of the activity in the newborn. Kinetic studies and inhibition characteristics with propylthiouracil, gold thioglucose, and T-4 indicated that the iodothyronine 5'-deiodinase in goat perirenal adipose tissue is of the type I form; there was no evidence for the type II enzyme. Affinity labeling of the iodothyronine 5'-deiodinase with bromoacetyl-rT(3) or bromoacetyl-T-4 was consistent with this view. In contrast to goats, only the type II enzyme is evident in rats and mice. It is concluded that the perirenal (brown) adipose tissue is likely to be a significant source of T-3 for other tissues in newborn goats and that there are major species variations in the form of iodothyronine 5'-deiodinase present in brown fat, with the goat exclusively exhibiting type I.

AB - The capacity to generate the active thyroid hormone 3,3',5-triiodothyronine (T-3) from thyroxine (T-4) has been assessed in the major adipose tissue depot (perirenal) of newborn goats through measurements of iodothyronine 5'-deiodinase activity. High activity of the enzyme was found in the perirenal adipose tissue. Developmental studies demonstrated that there was a rise, then a fall, in iodothyronine 5'-deiodinase activity (per milligram of protein) over the first week of postnatal life, with a further decline between the second and third weeks. At 3 wk of age, the activity per gram of tissue was only 2% of that of the newborn; however, because of age-related increases in the lipid content of the perirenal adipose tissue, the activity per cell (per mu g of DNA) at 3 wk of age was similar to 10% of the activity in the newborn. Kinetic studies and inhibition characteristics with propylthiouracil, gold thioglucose, and T-4 indicated that the iodothyronine 5'-deiodinase in goat perirenal adipose tissue is of the type I form; there was no evidence for the type II enzyme. Affinity labeling of the iodothyronine 5'-deiodinase with bromoacetyl-rT(3) or bromoacetyl-T-4 was consistent with this view. In contrast to goats, only the type II enzyme is evident in rats and mice. It is concluded that the perirenal (brown) adipose tissue is likely to be a significant source of T-3 for other tissues in newborn goats and that there are major species variations in the form of iodothyronine 5'-deiodinase present in brown fat, with the goat exclusively exhibiting type I.

KW - THERMOGENESIS

KW - BROWN ADIPOSE TISSUE

KW - THYROXINE

KW - TRIIODOTHYRONINE

KW - PERIRENAL ADIPOSE TISSUE

KW - IODOTHYRONINE 5'-DEIODINASE

KW - BROWN ADIPOSE-TISSUE

KW - I IODOTHYRONINE DEIODINASE

KW - PROTEIN DISULFIDE-ISOMERASE

KW - THYROID-HORMONE METABOLISM

KW - SELENIUM DEFICIENCY

KW - 5'-DEIODINASE ACTIVITY

KW - THYROXINE 5'-MONODEIODINATION

KW - TRIIODOTHYRONINE PRODUCTION

KW - UNCOUPLING PROTEIN

KW - 2 PATHWAYS

M3 - Article

VL - 267

JO - American Journal of Physiology

JF - American Journal of Physiology

SN - 0002-9513

IS - 1

ER -