Our data reveal that selection of enzymes for generating single cell suspensions from murine tissues influences detection of surface expression of antifungal CLRs. Using a method that most preserves receptor expression, we show that non-myeloid expression of antifungal CLRs is limited to MelLec on endothelial cells in murine mucosal tissues.
|Number of pages||4|
|Journal||European Journal of Immunology|
|Early online date||23 Jun 2021|
|Publication status||Published - Sep 2021|
- C-type lectin
- Endothelial cells
- Epithelial cells
- Immunity, Innate
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Iain Fraser Cytometry Centre
Andrea Holme (Manager), Linda Duncan (Senior Application Scientist), Ailsa Laird (Technician) & Kate Burgoyne (Technician)Institute of Medical Sciences
Research Facilities: Facility