Characterization of functional domains in the human coronavirus HCV 229E receptor

Andreas Kolb, J Maile, A Heister, S G Siddell

    Research output: Contribution to journalArticle

    34 Citations (Scopus)

    Abstract

    Human aminopeptidase N (hAPN or CD13) and porcine aminopeptidase N (pAPN) are functional receptors for human coronavirus (HCV) 229E and porcine transmissible gastroenteritis virus (TGEV), respectively, However, hAPN cannot function as a receptor for TGEV and pAPN cannot function as a receptor for HCV 229E, In this study, we constructed a series of chimeric hAPN/pAPN genes and expressed the corresponding proteins in transfected cells, Subsequently, we identified the chimeric proteins that can function as a receptor for HCV 229E, The results show that replacement of a small region of pAPN sequence (pAPN amino acids 255-348) with the corresponding hAPN sequence (hAPN amino acids 260-353) converts pAPN into a functional receptor for HCV 229E. The region of hAPN that we have defined in this way does not correspond to the region of pAPN that has been identified as essential for the TGEV-receptor interaction, We conclude that although both viruses use a homologous receptor protein, different regions of the protein are required to mediate susceptibility to infection with HCV 229E and TGEV.

    Original languageEnglish
    Pages (from-to)2515-2521
    Number of pages7
    JournalJournal of General Virology
    Volume77
    Issue number10
    Publication statusPublished - Oct 1996

    Keywords

    • aminopeptidase-N
    • glycoprotein
    • neutralization
    • resistance
    • sequence
    • binding
    • site

    Cite this

    Characterization of functional domains in the human coronavirus HCV 229E receptor. / Kolb, Andreas; Maile, J ; Heister, A ; Siddell, S G .

    In: Journal of General Virology, Vol. 77, No. 10, 10.1996, p. 2515-2521.

    Research output: Contribution to journalArticle

    Kolb, A, Maile, J, Heister, A & Siddell, SG 1996, 'Characterization of functional domains in the human coronavirus HCV 229E receptor', Journal of General Virology, vol. 77, no. 10, pp. 2515-2521.
    Kolb, Andreas ; Maile, J ; Heister, A ; Siddell, S G . / Characterization of functional domains in the human coronavirus HCV 229E receptor. In: Journal of General Virology. 1996 ; Vol. 77, No. 10. pp. 2515-2521.
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    AU - Kolb, Andreas

    AU - Maile, J

    AU - Heister, A

    AU - Siddell, S G

    PY - 1996/10

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    N2 - Human aminopeptidase N (hAPN or CD13) and porcine aminopeptidase N (pAPN) are functional receptors for human coronavirus (HCV) 229E and porcine transmissible gastroenteritis virus (TGEV), respectively, However, hAPN cannot function as a receptor for TGEV and pAPN cannot function as a receptor for HCV 229E, In this study, we constructed a series of chimeric hAPN/pAPN genes and expressed the corresponding proteins in transfected cells, Subsequently, we identified the chimeric proteins that can function as a receptor for HCV 229E, The results show that replacement of a small region of pAPN sequence (pAPN amino acids 255-348) with the corresponding hAPN sequence (hAPN amino acids 260-353) converts pAPN into a functional receptor for HCV 229E. The region of hAPN that we have defined in this way does not correspond to the region of pAPN that has been identified as essential for the TGEV-receptor interaction, We conclude that although both viruses use a homologous receptor protein, different regions of the protein are required to mediate susceptibility to infection with HCV 229E and TGEV.

    AB - Human aminopeptidase N (hAPN or CD13) and porcine aminopeptidase N (pAPN) are functional receptors for human coronavirus (HCV) 229E and porcine transmissible gastroenteritis virus (TGEV), respectively, However, hAPN cannot function as a receptor for TGEV and pAPN cannot function as a receptor for HCV 229E, In this study, we constructed a series of chimeric hAPN/pAPN genes and expressed the corresponding proteins in transfected cells, Subsequently, we identified the chimeric proteins that can function as a receptor for HCV 229E, The results show that replacement of a small region of pAPN sequence (pAPN amino acids 255-348) with the corresponding hAPN sequence (hAPN amino acids 260-353) converts pAPN into a functional receptor for HCV 229E. The region of hAPN that we have defined in this way does not correspond to the region of pAPN that has been identified as essential for the TGEV-receptor interaction, We conclude that although both viruses use a homologous receptor protein, different regions of the protein are required to mediate susceptibility to infection with HCV 229E and TGEV.

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    KW - site

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