Characterization of polymorphic microsatellite DNA markers in the ruff (Philomachus pugnax)

K. A. Thuman, F. Widemo, Stuart Brannon Piertney

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Abstract

Nine polymorphic microsatellite markers were isolated and characterized from the lek-breeding ruff (Philomachus pugnax) using an enrichment cloning procedure. The number of alleles resolved per locus ranged from five to 56, and observed heterozygosity ranged from 0.311 to 0.857 in a sample of 319 ruffs from Gotland, Sweden. Two loci were located on the Z chromosome, rendering all females hemizygotes. These markers are effective in the assignment of parentage and determining male mating success.

Original languageEnglish
Pages (from-to)276-277
Number of pages1
JournalMolecular Ecology Notes
Volume2
DOIs
Publication statusPublished - 2002

Keywords

  • enrichment
  • microsatellite
  • paternity
  • Philomachus
  • ruff
  • sex-linked

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