Chemical form and distribution of selenium and sulfur in the selenium hyperaccumulator Astragalus bisulcatus

I J Pickering, Carrie Wright, B Bubner, D Ellis, M W Persans, E Y Yu, G N George, R C Prince, D E Salt

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

In its natural habitat, Astragalus bisulcatus can accumulate up to 0.65% (w/w) selenium (Se) in its shoot dry weight. X-ray absorption spectroscopy has been used to examine the selenium biochemistry of A. bisulcatus. High concentrations of the nonprotein amino acid Se-methylseleno-cysteine (Cys) are present in young leaves of A. bisulcatus, but in more mature leaves, the Se-methylseleno-Cys concentration is lower, and selenate predominates. Seleno-Cys methyltransferase is the enzyme responsible for the biosynthesis of Se-methylseleno-Cys from seleno-Cys and S-methyl-methionine. Seleno-Cys methyltransferase is found to be expressed in A. bisulcatus leaves of all ages, and thus the biosynthesis of Se-methylseleno-Cys in older leaves is limited earlier in the metabolic pathway, probably by an inability to chemically reduce selenate. A comparative study of sulfur (S) and Se in A. bisulcatus using x-ray absorption spectroscopy indicates similar trends for oxidized and reduced Se and S species, but also indicates that the proportions of these differ significantly. These results also indicate that sulfate and selenate reduction are developmentally correlated, and they suggest important differences between S and Se biochemistries.

Original languageEnglish
Pages (from-to)1460-1467
Number of pages8
JournalPlant Physiology
Volume131
Issue number3
DOIs
Publication statusPublished - Mar 2003

Keywords

  • ray-absorption spectroscopy
  • non-accumulator plants
  • cancer prevention
  • ATP sulfurylase
  • selenate uptake
  • Indian mustard
  • leaf tissue
  • amino-acids
  • tolerance
  • metabolism

Cite this

Chemical form and distribution of selenium and sulfur in the selenium hyperaccumulator Astragalus bisulcatus. / Pickering, I J ; Wright, Carrie; Bubner, B ; Ellis, D ; Persans, M W ; Yu, E Y ; George, G N ; Prince, R C ; Salt, D E .

In: Plant Physiology, Vol. 131, No. 3, 03.2003, p. 1460-1467.

Research output: Contribution to journalArticle

Pickering, IJ, Wright, C, Bubner, B, Ellis, D, Persans, MW, Yu, EY, George, GN, Prince, RC & Salt, DE 2003, 'Chemical form and distribution of selenium and sulfur in the selenium hyperaccumulator Astragalus bisulcatus', Plant Physiology, vol. 131, no. 3, pp. 1460-1467. https://doi.org/10.1104/pp.014787
Pickering, I J ; Wright, Carrie ; Bubner, B ; Ellis, D ; Persans, M W ; Yu, E Y ; George, G N ; Prince, R C ; Salt, D E . / Chemical form and distribution of selenium and sulfur in the selenium hyperaccumulator Astragalus bisulcatus. In: Plant Physiology. 2003 ; Vol. 131, No. 3. pp. 1460-1467.
@article{487546879ef34a97bdb444676b8eedf5,
title = "Chemical form and distribution of selenium and sulfur in the selenium hyperaccumulator Astragalus bisulcatus",
abstract = "In its natural habitat, Astragalus bisulcatus can accumulate up to 0.65{\%} (w/w) selenium (Se) in its shoot dry weight. X-ray absorption spectroscopy has been used to examine the selenium biochemistry of A. bisulcatus. High concentrations of the nonprotein amino acid Se-methylseleno-cysteine (Cys) are present in young leaves of A. bisulcatus, but in more mature leaves, the Se-methylseleno-Cys concentration is lower, and selenate predominates. Seleno-Cys methyltransferase is the enzyme responsible for the biosynthesis of Se-methylseleno-Cys from seleno-Cys and S-methyl-methionine. Seleno-Cys methyltransferase is found to be expressed in A. bisulcatus leaves of all ages, and thus the biosynthesis of Se-methylseleno-Cys in older leaves is limited earlier in the metabolic pathway, probably by an inability to chemically reduce selenate. A comparative study of sulfur (S) and Se in A. bisulcatus using x-ray absorption spectroscopy indicates similar trends for oxidized and reduced Se and S species, but also indicates that the proportions of these differ significantly. These results also indicate that sulfate and selenate reduction are developmentally correlated, and they suggest important differences between S and Se biochemistries.",
keywords = "ray-absorption spectroscopy, non-accumulator plants, cancer prevention, ATP sulfurylase, selenate uptake, Indian mustard, leaf tissue, amino-acids, tolerance, metabolism",
author = "Pickering, {I J} and Carrie Wright and B Bubner and D Ellis and Persans, {M W} and Yu, {E Y} and George, {G N} and Prince, {R C} and Salt, {D E}",
year = "2003",
month = "3",
doi = "10.1104/pp.014787",
language = "English",
volume = "131",
pages = "1460--1467",
journal = "Plant Physiology",
issn = "0032-0889",
publisher = "American Society of Plant Biologists",
number = "3",

}

TY - JOUR

T1 - Chemical form and distribution of selenium and sulfur in the selenium hyperaccumulator Astragalus bisulcatus

AU - Pickering, I J

AU - Wright, Carrie

AU - Bubner, B

AU - Ellis, D

AU - Persans, M W

AU - Yu, E Y

AU - George, G N

AU - Prince, R C

AU - Salt, D E

PY - 2003/3

Y1 - 2003/3

N2 - In its natural habitat, Astragalus bisulcatus can accumulate up to 0.65% (w/w) selenium (Se) in its shoot dry weight. X-ray absorption spectroscopy has been used to examine the selenium biochemistry of A. bisulcatus. High concentrations of the nonprotein amino acid Se-methylseleno-cysteine (Cys) are present in young leaves of A. bisulcatus, but in more mature leaves, the Se-methylseleno-Cys concentration is lower, and selenate predominates. Seleno-Cys methyltransferase is the enzyme responsible for the biosynthesis of Se-methylseleno-Cys from seleno-Cys and S-methyl-methionine. Seleno-Cys methyltransferase is found to be expressed in A. bisulcatus leaves of all ages, and thus the biosynthesis of Se-methylseleno-Cys in older leaves is limited earlier in the metabolic pathway, probably by an inability to chemically reduce selenate. A comparative study of sulfur (S) and Se in A. bisulcatus using x-ray absorption spectroscopy indicates similar trends for oxidized and reduced Se and S species, but also indicates that the proportions of these differ significantly. These results also indicate that sulfate and selenate reduction are developmentally correlated, and they suggest important differences between S and Se biochemistries.

AB - In its natural habitat, Astragalus bisulcatus can accumulate up to 0.65% (w/w) selenium (Se) in its shoot dry weight. X-ray absorption spectroscopy has been used to examine the selenium biochemistry of A. bisulcatus. High concentrations of the nonprotein amino acid Se-methylseleno-cysteine (Cys) are present in young leaves of A. bisulcatus, but in more mature leaves, the Se-methylseleno-Cys concentration is lower, and selenate predominates. Seleno-Cys methyltransferase is the enzyme responsible for the biosynthesis of Se-methylseleno-Cys from seleno-Cys and S-methyl-methionine. Seleno-Cys methyltransferase is found to be expressed in A. bisulcatus leaves of all ages, and thus the biosynthesis of Se-methylseleno-Cys in older leaves is limited earlier in the metabolic pathway, probably by an inability to chemically reduce selenate. A comparative study of sulfur (S) and Se in A. bisulcatus using x-ray absorption spectroscopy indicates similar trends for oxidized and reduced Se and S species, but also indicates that the proportions of these differ significantly. These results also indicate that sulfate and selenate reduction are developmentally correlated, and they suggest important differences between S and Se biochemistries.

KW - ray-absorption spectroscopy

KW - non-accumulator plants

KW - cancer prevention

KW - ATP sulfurylase

KW - selenate uptake

KW - Indian mustard

KW - leaf tissue

KW - amino-acids

KW - tolerance

KW - metabolism

U2 - 10.1104/pp.014787

DO - 10.1104/pp.014787

M3 - Article

VL - 131

SP - 1460

EP - 1467

JO - Plant Physiology

JF - Plant Physiology

SN - 0032-0889

IS - 3

ER -