Chlamydia species-dependent differences in the growth requirement for lysosomes

Scot P Ouellette, Frank C Dorsey, Simon Moshiach, John L Cleveland, Rey A Carabeo

Research output: Contribution to journalArticle

46 Citations (Scopus)
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Abstract

Genome reduction is a hallmark of obligate intracellular pathogens such as Chlamydia, where adaptation to intracellular growth has resulted in the elimination of genes encoding biosynthetic enzymes. Accordingly, chlamydiae rely heavily on the host cell for nutrients yet their specific source is unclear. Interestingly, chlamydiae grow within a pathogen-defined vacuole that is in close apposition to lysosomes. Metabolically-labeled uninfected host cell proteins were provided as an exogenous nutrient source to chlamydiae-infected cells, and uptake and subsequent labeling of chlamydiae suggested lysosomal degradation as a source of amino acids for the pathogen. Indeed, Bafilomycin A1 (BafA1), an inhibitor of the vacuolar H(+)/ATPase that blocks lysosomal acidification and functions, impairs the growth of C. trachomatis and C. pneumoniae, and these effects are especially profound in C. pneumoniae. BafA1 induced the marked accumulation of material within the lysosomal lumen, which was due to the inhibition of proteolytic activities, and this response inhibits chlamydiae rather than changes in lysosomal acidification per se, as cathepsin inhibitors also inhibit the growth of chlamydiae. Finally, the addition of cycloheximide, an inhibitor of eukaryotic protein synthesis, compromises the ability of lysosomal inhibitors to block chlamydial growth, suggesting chlamydiae directly access free amino acids in the host cytosol as a preferred source of these nutrients. Thus, chlamydiae co-opt the functions of lysosomes to acquire essential amino acids.
Original languageEnglish
Article numbere16783
Number of pages16
JournalPloS ONE
Volume6
Issue number3
DOIs
Publication statusPublished - 8 Mar 2011

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Chlamydia
lysosomes
Lysosomes
Pathogens
Nutrients
Acidification
Growth
Vacuolar Proton-Translocating ATPases
Amino Acids
Cathepsins
Gene encoding
Essential Amino Acids
Cycloheximide
Labeling
pneumonia
acidification
pathogens
Food
nutrients
Proteins

Keywords

  • animals
  • positive transcriptional elongation factor B
  • amino acids
  • autophagy
  • humans
  • inclusion bodies
  • protein processing, post-translational
  • macrolides
  • cycloheximide
  • mice
  • models, biological
  • cattle
  • gene knockdown techniques
  • cytosol
  • serum albumin, bovine
  • species specificity
  • lysosomes
  • chlamydia

Cite this

Ouellette, S. P., Dorsey, F. C., Moshiach, S., Cleveland, J. L., & Carabeo, R. A. (2011). Chlamydia species-dependent differences in the growth requirement for lysosomes. PloS ONE, 6(3), [e16783]. https://doi.org/10.1371/journal.pone.0016783

Chlamydia species-dependent differences in the growth requirement for lysosomes. / Ouellette, Scot P; Dorsey, Frank C; Moshiach, Simon; Cleveland, John L; Carabeo, Rey A.

In: PloS ONE, Vol. 6, No. 3, e16783, 08.03.2011.

Research output: Contribution to journalArticle

Ouellette, SP, Dorsey, FC, Moshiach, S, Cleveland, JL & Carabeo, RA 2011, 'Chlamydia species-dependent differences in the growth requirement for lysosomes', PloS ONE, vol. 6, no. 3, e16783. https://doi.org/10.1371/journal.pone.0016783
Ouellette SP, Dorsey FC, Moshiach S, Cleveland JL, Carabeo RA. Chlamydia species-dependent differences in the growth requirement for lysosomes. PloS ONE. 2011 Mar 8;6(3). e16783. https://doi.org/10.1371/journal.pone.0016783
Ouellette, Scot P ; Dorsey, Frank C ; Moshiach, Simon ; Cleveland, John L ; Carabeo, Rey A. / Chlamydia species-dependent differences in the growth requirement for lysosomes. In: PloS ONE. 2011 ; Vol. 6, No. 3.
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